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Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.
Lucerne is a fodder perennial plant from the Fabaceae family grown under field conditions as feed for various groups of animals. This paper is concerned with the effect of electromagnetic stimulation of lucerne seed on the yield, yield parameters, and content of photosynthetic pigments in 1-2- and 5-6- year old lucerne plants. In 2012-2013 a field experiment was conducted with 5-6-year old lucerne, which was initiated in 2008. Another experiment was started in 2012 and conducted on 1-2-year old lucerne. The research material were seeds of hybrid lucerne (Medicago x varia T. Martyn) variety Radius and sowing lucerne (Medicago sativa L.) variety Ulstar. Before sowing the seeds were stimulated with the following combinations of physical factors: C – control (untreated sample), L – laser light with a surface power density of 6 mW cm–2 during 3-fold free fall, F – alternating magnetic field with an induction of 30 mT and exposure time of 30 s; L + F – laser light and magnetic field in the above doses. Electromagnetic stimulation contributed significantly to an increase in the number of shoots per 1 m2 as compared to the control. No significant difference in the weight of a single shoot was observed between the experimental combinations. 5- 6-year old lucerne was characterised by a statistically greater shoot mass as compared with 1-2-year old plants. Statistical analysis showed that the highest yield increase was observed for the samples where seeds were stimulated with laser light and both laser light and magnetic field, relative to the control. The best results concerning concentrations of chlorophyll a and b and carotenoids were observed for alternating magnetic field, where the increase amounted to 9, 11 and 13.0%, respectively, as compared to the control.
Pots experiment was carried out during season 2017 at greenhouse of the Agric. Bot. Dep., Fac. of Agric., Zagazig Univ., Egypt to evaluate the effect of glycine betaine (GB) application under salinity stress (50 and100 mM Nacl) on growth, physio-chemical analysis and yield of snap bean cv. Bronco. A complete randomized blocks design was used in this search with three replications. Growth parameters, chlorophyll content and green pod yield were significantly decreased with subjecting plants to NaCl. However foliar application of GB detoxified the stress generated by NaCl and significantly improved the above mention parameters. Salinity stress increased the electrolyte leakage (EL) and decreased membrane stability index (MSI) and relative water content (RWC). While foliar application of GB was improved MSI and RWC and minimized EL. Proline content and antioxidant enzymes significantly increased in the response to NaCl stress as well as GB application.
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