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Yeast-like fungi isolated in students

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The occurrence of yeast-like fungi in the most important infection portals of the respiratory system in 200 randomly chosen students of biology and veterinary medicine was examined. The students come into direct contact with plants and animals that may be colonised by fungi belonging to various systematic groups. nine species of yeast-like fungi, including 7 species determined in the biologists, were recorded in the subjects. Candida tropicalisand C.albicanswere the most frequently isolated fungi. The greatest number of fungi was isolated from the oral cavity (124 isolates), fewer from the throat (79 isolates), and the smallest number from the nose (8 isolates). Fungi occurred more frequently in autumn and slightly less frequently in spring, and were isolated more frequently from women than from men.
A total of 69 isolates of yeasts were recorded in the indoor air of the school buildings: 43 in heated rooms and 26 in unheated rooms. Perfect stages prevailed. Fungi isolated in our study belonged to 39 species. These were mostly monospecific isolates although five two-species isolates were noted. Differences in the properties of physiological characters of fungi isolated in both study seasons were observed. As indoor and outdoor air does not mix during the heating season, a specific substrate for prototrophic, non-fermenting yeastlike fungi forms. Acid production allows fungi to dissolve inorganic compounds in building structures and to release needed microcomponents. Abilities to produce carotenoid pigments are clearly promoted in yeast-like fungi living indoor. This may be related to the accumulation of compounds that are indirect stages in the cycle of biosynthesis of carotenoids or a surplus of oxidizing compounds.
Undertaken studies on diversity and prevalence of yeast-like fungi aimed at presenting the role of a hydrophyte treatment plant in cleaning municipal wastewater from potentially pathogenic fungi. One hundred and six isolates of fungi were obtained. The highest diversity of species was found in the sedimentation tank and in I and II reed beds. The dominating species were: Trichosporon beigelii, Candida albicans, Candida glabrata, Candida dubliniensis, Candida krusei, Candida utilis and Candida lipolytica – isolated also from hospitalized patients. The number of fungal cells decreased progressively from uncountable in the sedimentation tank to 2,060 cfu/dm³ in the polishing pond. Reduction of the number and changes in species composition of yeasts like fungi take place in the hydrophyte treatment plants.
The study is part of hydromycological investigations of man-affected water bodies in Olsztyn. Our results show that yeast-like fungi are permanent components in three selected lakes (lakes Tursko, Długie and Skanda). Their abundance and species composition depend on the trophic state of each lake.
The mycobiota responsible for the development of pathological changes of the skin and its adnexa in patients presenting at the Specialist Regional Hospital, Łódź, with suspected superficial mycosis between 01 May 2003 and 30 April 2005 is analyzed. In total of 2144 isolations 39.96% were dermatophytes, 39.39% were yeast-like fungi and 20.65% were moulds. Candida albicans was the most frequently diagnosed species in fallowed by Trichophyton rubrum.
A high incidence of ample diseases with a complicated etiology, including mycoses of various origins, determine the implementation of unconventional methods, including hirudotherapy, to their treatment. The study was aimed at assessing the mycological purity of medical leech body covers, their jaws and gastrointestinal systems – oesophagus, middle and posterior intestines, and the purity of water from an aquarium they were kept in. The experimental material were 20 European medical leeches (Hirudo medicinalis) and water originating from their breeding. Fungi were obtained according to our own procedures and mycological diagnostics was conducted following standard procedures applied in mycological laboratories. In total, 22 species of fungi belonging to 13 genera were isolated. The material studied was found to contain fungi classified as potential pathogens: Candida albicans, Candida guilliermondii, Candida krusei and Candida tropicalis, as well as numerous saprotrophs with a decreased pathogenicity potential. In view of the results obtained in this study, and a prospective application of hirudotherapy, a question may arise as to whether sterility of cultures and health status of leeches are sufficient and safe for patients. Isolation of a relatively high number of fungi considered pathogenic from leeches poses a potential hazard for people who could have contact with non-sterile therapeutic material, which in this case leeches are.
The goal of the study was to compare enzymatic activities of Candida albicans, Candida guilliermondii and Rhodotorula glutinis collected from different aquatic ecosystems of Olsztyn. The highest enzymatic activity was observed in isolates obtained from astatic reservoirs (isolates of Candida albicans and Rhodotorula glutinis produced the complete set of 19 hydrolases tested). A slightly lower enzymatic activity was observed in isolates from a lake and the lowest in those from a river. The widest enzymatic spectrum as well as high and very high enzymatic activity (20-45 nmols) were found in isolates of Rhodotorula glutinis originating from the lake and astatic reservoirs and in Candida guilliermondii isolates from the lake. Both species are reported by hydrologists as important in the processes of waters self-purification.
This paper reviews the results of an extensive monitoring study, spanning 20-years of observation from three medical centres in Olsztyn on the dynamics and species diversity of fungi most frequently colonizing the respiratory and digestive systems of humans. The experimental materials were swabs and specimens from the gastrointestinal tract, swabs from the oral cavity and pharynx, as well as sputum and bronchial fluid from the respiratory system. The biological material was subjected to routine mycological diagnostics, taxonomic determination and identification. In total, 41 species of yeast and yeast-like fungi were isolated, including 34 from the respiratory and 25 from the digestive system. In the last decade, a significant increase has been noticed in the counts of fungi, especially the gastrointestinal tract, reported from people. As many as 18 species were isolated from both systems – they were predominated by fungi of the genus Candida and their perfect forms. Worthy of notice are also frequent isolations of yeast (Saccharomyces spp.) and detection of an endemic species, Paracoccidioides brasiliensis, in the respiratory system, and of the sexual stages of Rhodosporidium diobovatum and Rhodosporidium kratochvilovae from the gastrointestinal tract.
A total of 111 isolates of yeast-like fungi and yeasts belonging to 40 species of 19 genera were identified in indoor air and outdoor air. Only one species, Kluyveromyces marxianus, was recorded in both types of air and seasons (spring and autumn). Kluyveromyces lactis and Yarrowia lipolytica, a species having the greatest symbiotic abilities, dominated in indoor air and outdoor air, respectively. Intensely used rooms, especially those with limited access of air, have the broadest range of species of yeast-like fungi. A comparison of both habitats shows that school rooms pose a greater epidemiological risk of yeast-like infections than outdoor air. The indoor as well as outdoor mycobiota undergoes phenological changes although it is determined by other biotic and abiotic factors.
The aim of our study was to determine species biodiversity of yeasts and yeast-like fungi in the stratum of surface water of Lake Tyrsko, as well as to evaluate the condition of waters of that lake based on the isolated species of fungi. The study was carried out from June 2007 to June 2008. The isolation and determination of the number of fungal colonies were conducted with the use of the membrane filter method. Fungal diagnostics were conducted based on the morphological and biochemical characteristics of the fungi. These characteristics were additionally used for taxonomic identification. In the course of the study we isolated 56 species of yeasts and yeast-like fungi belonging to 26 genera, with the predominating genus being Kluyveromyces (13 species), followed by less frequent genera: Candida (8 species), and Debaryomyces (7 species). The highest number of isolates were obtained in the spring (51), fewer in the summer (36), and the lowest in autumn (15). Species indicatory of the self-purifying process of the water examined were frequently isolated (Debaryomyces hansenii, Pichia membranifaciens). Results obtained in the study confirm suggestions of other authors that yeasts and yeast-like fungi may be useful as indicatory organisms showing the extent and character of water contamination and as bioindicators of sanitary and hygienic evaluation of water.
Yeast-like fungi are the most common cause of fungal infections in humans. Actually, in the age of opportunistic infections and increasing resistance, development of modern antifungal agents becomes a very important challenge. This paper describes synthesis and antimicrobial assay of four naturally occurring peptide antibiotics (aurein 1.2, citropin 1.1, temporin A, uperin 3.6) and three chemically engineered analogues actually passing clinical trials (iseganan, pexiganan, omiganan) against Candida strains isolated from patients with infections of the oral cavity or respiratory tract. The peptides were synthesized using solid-phase method and purified by high-performance liquid chromatography. Biological tests were performed using the broth microdilution method. The antifungal activity of the peptide antibiotics was compared to that of nystatin and amphotericin B. We found synthetic peptides to be generally less potent than amphotericin B or nystatin. However, some of the naturally occurring peptides still retained reasonable antifungal activities which were higher than these of iseganan, pexiganan or omiganan. We think that the naturally occurring peptide antibiotics included in our study can be a good matrix for development of novel antifungal compounds.
In 2008 and 2009 the effect of two cultivation systems (conventional and ecological) on population of yeasts and mycelial fungi colonizing grain of two winter wheat cultivars (Bogatka and Legenda) was studied. In 2008 only grain of cv. Bogatka grown under the conventional system was colonized by significantly higher number of fungi than grain under the ecological system. In 2009 with frequent rainfall grain of both cultivars grown under the ecological system was colonized by a higher number of fungi as compared to the conventional system. In 2008 more isolates of Fusarium poae and F. sporotrichioides were obtained from grain grown under the conventional than under the ecological system, but in 2009 the opposite was generally true.
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