Wyniki wyszukiwania

1

Alpha-ketoglutarate dehydrogenase and lipoic acid synthase are important for the functioning of peroxisomes of Saccharomyces cerevisiae

100%

Smaczynska-de R. I., Migdalski A., Rytka J.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 2

A method was devised to search for yeast mutants impaired in peroxisome functioning, indicating cross-talk between metabolic pathways. Two mutants were isolated; they are impaired in oleate utilisation and carry mutations in the KGD1 and LIP5 genes encoding the E1 component of the mitochondrial α-ketoglutarate dehydrogenase complex and lipoic acid synthase, respectively. The results presented indicate that the Kgd1 and Lip5 proteins are important for the expression of genes encoding peroxisomal matrix proteins, although they are not necessary for the biogenesis of this cellular compartment.

2

The physiological and morphological phenotype of a yeast mutant resistant to the quaternary ammonium salt N-[dodecyloxycarboxymethyl]-N,N,N-trimethyl ammonium chloride

84%

Oblak E., Gamian A., Adamski R., Ulaszewski S.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 2

We investigated the action of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on Saccharomyces cerevisiae yeast cells. Changes in the yeast cell ultrastructure were confirmed by electron microscopy. We treated resistant mutant cells with QAS, and confirmed destruction of the mutant cytoplasm, an increase in the thickness of the cell wall, separation of the cell wall from the cytoplasm, and the accumulation of numerous lipid droplets. We also observed a relatively high production of lipids in the cells of the parental wild-type strain Σ1278b and in its IM-resistant (IMR) mutant in the presence of the QAS. The IMR mutant showed increased sensitivity to CaCl2 and SDS, and resistance to ethidium bromide, chloramphenicol, erythromycin and osmotic shock. It also tolerated growth at low pH. We suggest that the resistance to IM could be connected with the level of permeability of the cell membrane because the IMR mutant was sensitive to this compound in vivo in the presence of SDS and guanidine hydrochloride, which cause increased permeability of the cell plasma membrane.

3

The effect of synthetic polyanion on diffusion of adenine nucleotides through outer membrane of porin-free yeast mitochondria

84%

Stobienia O., Kmita H., Michejda J.

Biological Bulletin of Poznań

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1999

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tom 36

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nr 1

4

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function

51%

Lenart J., Pikula S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes.

Ograniczanie wyników

3 Cellular and Molecular Biology Letters

1 Biological Bulletin of Poznań

1 Adamski R.

1 Gamian A.

1 Kmita H.

1 Lenart J.

1 Michejda J.

1 Migdalski A.

1 Oblak E.

1 Pikula S.

1 Rytka J.

1 Smaczynska-de R. I.

1 Stobienia O.

1 Ulaszewski S.

1 2010

1 2004

1 1999

1 1998

Alpha-ketoglutarate dehydrogenase and lipoic acid synthase are important for the functioning of peroxisomes of Saccharomyces cerevisiae

The physiological and morphological phenotype of a yeast mutant resistant to the quaternary ammonium salt N-[dodecyloxycarboxymethyl]-N,N,N-trimethyl ammonium chloride

The effect of synthetic polyanion on diffusion of adenine nucleotides through outer membrane of porin-free yeast mitochondria

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function