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White yam was fortified with cassava and plantain flours to develop a traditional food called ‘amala’. The fortified amala were subjected to organoleptic and chemical evaluations. Results showed that the protein and water absorption capacity of the fortified amala increased significantly. The panelists found yam fortified with plantain more acceptable. The samples stored in the refrigerator produced low pH values, remained fresh and maintained their attractive organoleptic attributes for a longer period than those stored at the room temperature.
Bacillus subtilis strains (CM1-CM5) isolated from culturable (cowdung microflora were investigated for indole-3-acetic acid (IAA) production in nutrient broth (NB). All the strains tested produced IAA in NB; albeit in very low concentrations (0.09-0.37 mg/l). The addition of L-tryptophan (0.1-1.0 g/l) into NB substantially enhanced IAA production (6.1-31.5 folds) indicating that L-tryp-tophan was the precursor for IAA biosynthesis by these bacterial strains. Maximum IAA production was observed after 8 days of incubation (in late stationary phase of bacterial growth). The variation in IAA production was attributed to the genetic make up of these strains as evaluated by RAPD analysis of these isolates and B. subtilis type strain MTCC 441. Application of B. subtilis suspension (8 x 10⁹ CFU/ml) on the surface of yam (Dioscorea rotundata L.) minisetts increased the number of sprouts, roots and shoots length, root and shoot fresh weights and root: shoot ratio over those minisetts not treated with bacterial suspension. Fresh cowdung slurry treatment on yam minisetts also produced similar results as obtained with B. subtilis application.
A survey was conducted to determine the types, frequency and population of plant parasitic nematodes associated with the soils and roots of Yam (Dioscorea species) in all the Local Government Areas of Ogun and Osun States of Nigeria using random sampling soil and root and pie pan modification of Baerman funnel for plant parasitic nematode extraction. Ten and nine genera of plant parasitic nematodes were encountered both from the soils and root samples from the two States. Plant parasitic nematodes recovered included Scutellonema spp., Meloidogyne spp., Pratylenchus spp., Trichodorus spp., Helicotylenchus spp., Radopholus spp., Longidorus spp., Xiphinema spp., Rotylenchulus spp and Aphelenchoides species. Scutellonema spp., Meloidogyne spp., and Pratylenchus spp were most widely distributed with frequency ratings of 70, 65 and 60% respectively in soil samples from Ogun State and in the root samples the three genera predominated with 60, 55 and 45% frequency ratings respectively. Meloidogyne spp., Scutellonema spp., and Pratylenchus spp were most widely distributed with frequency ratings of 65, 45 and 35% respectively in soil samples from Osun State and in the root samples the three genera predominated with 55, 35 and 35% frequency ratings respectively.
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