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The aim of the study was to establish the possibility of inducing estrus in Leine ewes apart from the reproduction period by means of gestagene (Chronogest-Intervet) and a double injection of PMSG (Gonadotropin-Biovet) at priming - 50 iu and stimulating - 500 iu doses, and to compare the effects of estrus synchronization with the method used until now in which one injection of PMSG was applied. The efficacy of estrus synchronization confirmed by lambing in the group in which priming was used was 55.9%, and in the group in which only one stimulating dose of PMSG was applied was 58.3%. It can be assumed that the use of small priming dose of PMSG during estrus synchronization did not affect the efficacy of estrus synchronization and ovulation. In the group with implanted gestagene, only 8% of ewes lambed, indicating a very strong relationship of reproduction period with the season in Leine sheep. Out of season, the lambing period effectively changed without PMSG stimulation. For a preliminary evaluation of the efficacy of estrus synchronization and lambing the determination of the level of progesterone appeared to be useful mainly to confirm lambing (85.8-89.6% concordance).
The aim of the study was to establish the correlation between energy balance disturbances and the effectiveness of the induction of ovulation in mares in foal heat. The investigation was carried out on 90 thoroughbred mares, which were divided in two groups on 45 animals: A - induced ovulation, B - spontaneous ovulation. During foal heat, concentrations of glucose, β-hydroxybutyrate and free fatty acids were determined. On the basis of their biochemical status mares were divided in two subgroups: with and without energy balance disturbances. At the follicle diameter of 40 mm the mares received either 40 µg GnRH- analogue (buserelin) or 10 ml saline solution. Ovulation occurred within 48 hours in 31% mares with energy balance disturbances and 62.5% without energy balance disturbances (p ≤ 0.001). Fertility performance was significantly higher in mares without energy balance disturbances (p ≤ 0.001).
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