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Staphylococcus aureus is a very important pathogen in humans about 20%of all bacterial infections are caused by S. aureus. Because the staphylococcal sensitivity patterns have changed, the aim of this study was to investigate the current susceptibility of the S. aureus strains to 9 antibiotics: penicilin (P), amoxacillin / clavulanic acid (AMC), erythromycin (E), cortimoxazole (SXT), tetracycline (T), chloramphenicol (C), mupirocin (MUP), gentamicin (Ge) and vancomycin (Va). Susceptibility testing was performed by disc diffusion technique, by the procedure outlined by the National Committe for Clinical Laboratory Standards (NCCLS). Susceptibility to methicillin was determined using 5 µm methicillin disks, ß-lactamase production in the penicillin - resistant strains was detected with nitrocefin impregnated disk (Cefinase, BBL Microbiology system). The microbiological characteristics of the samples: 338 of the isolates were derived, from noses and the throaths 382 - from surgical wound secretions. The analysis of the results showed that most S. aureus strains (82,8%) are penicillin resistant and ß-lactamase producing 13,5% of all strains were methicillin - resistant. 16,5% - were erythromycin resistant; 3,2% - were cotrimoxazolc resistant; 51,4% - were mupirocin resistant; 20,6% - were gentamicin resistant. All of the strains were vancomycin sensitive.
An increasing number of S. suis strains showing resistance to commonly used antibiotics have been identified over the last years. The aim of the study was to determine the antibiotic susceptibility of Polish field S. suis strains using the broth microdilution method. The following antibiotics were included in the test: penicillin, ampicillin, ceftiofur, gentamicin, neomycin, spectinomycin, erythromycin, tylosin, tiamulin, chlotretracycline, oxytetracycline, enrofloxacin, sulphadimetoxin, sulphachloropyridazine, sulphathiazole, sulphamethoxazole with trimethoprim and florphenicol. The S. suis strains tested were collected between 1996-2005 from pigs indicating nervous symptoms, respiratory tract disorders and/or arthritis. The necropsy hyperaemia of meninges revealed inflammation of lungs and joints and the presence of serofibrinous exudate on the pericardium. The results of the study demonstrate the high level of susceptibility of tested strains to b-lactams (about 100% of susceptible strains), florphenicol (98%), gentamycin and a combination of sulphamethoxazole and trimethoprim (95%), enrofloxacin and spectinomycin (90.37%). A large percentage of strains were resistant to chlortetracycline (49%), oxytetracycline (40%), erytromycin and tylosin (50%) and to sulphonamides (50.37%). Most strains were susceptible to the lowest concentrations of b-lactams and sulphametoxazole with trimethoprim. The greatest antimicrobial activity was obtained for florphenicol, enrofloxacin, spectinomycin and gentamicin when the highest concentration of antibiotics was used in the study. The presented study confirms the high susceptibility of Polish field S. suis strains to the antibiotics recommended for treating streptococci. Positive therapeutic effects should be expected after using b-lactams, florphenicol and sulphonamides with trimethoprim, gentamicin and fluoroquinolones.
W pracy dokonano analizy lekooporności szczepów Escherichia coli i Klebsiella pneumoniae izolowanych od pacjentów. Wykonano testy określające zdolność bakterii do produkcji ß-laktamaz o rozszerzonym spektrum substratowym (ESBL).
Przy oznaczaniu wrażliwości Staphylococcus cohnii na antybiotyki ß-lakta- mowe zastosowanie warunków hodowli stymulujących mechanizmy oporności sprzyja eliminacji błędnych wyników, niezgodnych z dobrze sprawdzającymi się wynikami oznaczania meticylinooporności w krążkowym teście z cefoksytyną (FOX30).
295 ejaculates of 150 stallions of various breeds (full English, Great Polish, Hannower and Polish ponny) used for mating in 17 horse breeding centres in Poland were examined bacteriologically. More than 23% of ejaculates were contaminated with facultative pathogenic bacteria, mostly by Pseudomonas aeruginosa (10% of samples) sensitive to streptomycin (100%) and neomycin (8%). Sir. zooepidemicus was also isolated (9.5%), sensitive mostly to chloramphenicol (73%), penicillin (64%) and oxytetra- cycline (50% of strains).
W ciągu 4 miesięcy badań wyhodowano 111 szczepów bakterii Gram-dodat- nich od pacjentów z oddziału chirurgii ogólnej. Wśród wyizolowanych drob¬noustrojów dominowały gronkowce (49 szczepów) i enterokoki (44 szczepy). Wykryto 33 szczepy metycylinoopornych gronkowców. Penicyliny z inhibi¬torami ß-laktamaz oraz karbapenemy wykazały wysoką aktywność in vitro wobec klinicznych szczepów bakterii Gram-dodatnich z wyjątkiem E. fae- cium.
Zbadano wrażliwość 43 szczepów Klebsiella pneumoniae wytwarzających ESBL wobec sanfetrinemu. Uzyskane wyniki porównano z wynikami dla imi- penemu (MIC50, MIC90). Wszystkie izolaty były wrażliwe na imipenem i cefe- pim, a oporne na gentamycynę, amikacynę i trimetoprim/sulfametoksazol.
The honey bee disease American foulbrood (AFB) is a serious problem since its causative agent (Paenibacillus larvae) has become increasingly resistant to conventional antibiotics. From the year 2000 the number of isolates of P. larvae resistant against oxytetracycline (OTC) has increased. Therefore, since 2000 many antibiotics, chemotherapeutic propolis and plant extracts were tested in vitro and in vivo against P. larvae. The most active in the in vitro tests appeared to be deoxycycline, minocycline, rifampicin and lincomycin. Among the 35 examined antibacterial compounds active against P. larvae were narasin, salinomycin and tylosine. In many countries tylosine, tilomycosis have been recommended for the control of AFB, while microsamicine was recommended in Japan. The studies with plain essential oils did not succeeded. The propolis extracts from the various parts of the world showed significant inhibition of P. larvae. Due to the evolution of the resistance of P. larvae to conventional antibiotic treatments, the research on normal bee microflora (Bacillus, Paenibacillus, and Brevibacillus) is an important step in identifying possible new active tools to treat AFB in honey bee colonies.
The aim of the study was to determine the activity of four beta-lactam antibiotics against nosocomial strains of gram-negative bacilli. Two antibiotics combined with beta-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in the following wards: surgery and intensive care unit of State Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 E and ID 32 GN strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB G- and ATB PSE strips. The susceptibility of the strains to imipenem, meropenem, timentin and tazocin was tested by disc-diffusion method. 157 strains of gram-negative bacilli were cultured. 100 strains were isolated from patients hospitalized in surgical ward and 57 strains from patients hospitalized in ICU. Nonfermenting rods dominated among isolated strains -91. The results obtained indicate that multiresistant gram-negative rods causing serious thera­peutic problems are often isolated from clinical specimens. The contribution of nonfermenting rods, especially Pseudomonas spp. and Atinetobacter spp. to the etiology of infections in hospitalized patients has increased. Infections caused by these strains are difficult to cure. Tazocin and carbapenems (imipenem and meropenem) are highly active in vitro against the examined strains of gram-negative bacilli.
In the action undertaken for evaluating of the reliability of serological tests for M. pneumo­niae infection 33 laboratories of the Sanitary Epidemiological Stations participated. Every laboratory had to determine twice at an interval of 2-4 weeks the level of mycoplasma antibodies by the complement fixation test in serum samples divided into 4 groups: sera not containing these antibodies-titre < 5, or containing them in titres of 60, 120 and 320. The correct results of the determinations were obtained in 27 laboratories (81.8%) for samples without M. pneumoniae antibodies, and in 22 (66.6%) and 14 (42.4%) for samples with titres of 60 and 120, and 320 respectively. Only 4 laboratories (12.1%) obtained correct results of these determinations for every sample and in both testing series. In these series considered separately correct results were obtained in 9 (27.3%) and 8 (24.2%) laboratories. Faulty results in all samples in both testing series were reported from 2 (6.1%) laboratories. In the individual series all false results were obtained in 4 (12.1%) and 3 (9.1%) laboratories. The study showed that for raising of the quality and reliability level of serological investigations for M. pneumoniae infection a permanent practice should be periodic training of laboratory workers and frequently repeated interlaboratory controls of the reliability of test results.
The aim of the study was the genetic identification and evaluation of properties of field isolates of A. pyogenes. Samples of internal organs as well as pathologically changed joints taken from dead pigs bred in Poland were used for testing. The isolation of bacteria was carried out according to standard microbiological methods. Properties of bacteria were evaluated based on their color, size and shape on the agar plates and in smears stained with the Gram method as well as on results of biochemical tests. Genetic identification of isolates based on results of the elaborated PCR test. Sensitivity to the most commonly used chemotherapeutics was evaluated by the disc diffusion test. Monocultures of transparent, small bacteria of pinhead size, surrounded by large hemolysis zone, Gram positive, fermented with acid production of glucose, fructose, galactose, lactose, cellobrose, trecholose, maltose, melarytose, mannose, glikogen, xylose and starch, of proteolytic activity, were evidenced. Tested A. pyogenes isolates were 100% sensitive to a wide spectrum of beta-lactames, with the exception of penicillin, for which 5% of the strains were resistant. One hundred percent of isolates were sensitive to oxytetracycline, doxycyclin, enrofloxacin, tylosin and florfenicol and 95% of them were sensitive to tetracyklines, cephalosporin and Linco-Spectin. A high percentage (90%) of sensitivity was registered for tiamulin, tulatromycyn, gentamicin and norfloxacin. The highest resistance was evidenced in the case of neomycin (47% resistant strains) and sulphonamides with trimetoprimem (45% of resistant strains). In PCR the presence of genetic material of A. pyogenes was detected in the case of 56 tested strains (98.2%).
Celem pracy była analiza profilu drobnoustrojów izolowanych od biorców nerki. Ogółem pobrano 140 próbek od 53 pacjentów i 40 próbek płynu konserwującego Euro-Colins. Obecność drobnoustrojów wykazano w 125 (69,4%) próbkach. Bakterie Gram-dodatnie stanowiły 58,4%, Gram-ujemne - 34,2%, a grzyby – 7,4%.
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