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The dry matter content of 245 faecal samples of 81 species of captive wild ruminants was investigated. Samples were taken from 10 different zoos from temperate climate during the winter months. All animals had ad libitum access to drinking water. Species were classified as frugivores (FR, n = 5), browsers (BR, n = 16), intermediate feeders (IM, n = 35), and grazers (GR, n = 25). While no difference was observed in the average faecal dry matter content between the feeding types, the range of dry matter contents increased continually from FR, BR, IM to GR, ie both the driest and the wettest faeces of this study were produced by grazing ruminants. As it has been shown that faecal dry matter content is a function of the length of the colon descendens of a ruminant species, these results can be interpreted as an indication of a relatively limited variation in anatomical design in this respect in the evolutionary older FR and BR, and a relatively larger variation in anatomical design - and probably niche adaptation - in IM and GR. This corresponds to the greater range of habitat niches that IM and ÔR manage to occupy. Due to this increased variation in the anatomical design of the lower hindgut, GR are regarded as "morphophysiologically progressive rumi­nants", in contrast to BR which are regarded as "morphophysiologically conservative ruminants".
Primary bovine thyroid cell cultures and IB-RS-2 continuous cell line were used for foot-and-mouth disease virus (FMDV) isolation. In both cell culture systems, all tested samples gave positive results and the specificity of isolated virus was confirmed by the Ag-FLISA. Results of virus isolation test agreed with those obtained by RT-PCR and rRT-PCR, which enabled detection of the genetic material of FMDV. This indicates a high and comparable sensitivity of the applied diagnostic assays, which permit a reliable detection of FMDV in biological material.
Between 2003-2007, abomasa of 91 European bison (Bison bonasus), 4 red deer (Cervus elaphus) and 2 roe deer (Capreolus capreolus) shot in the Białowieża Primeval Forest (Poland) were examined for worms presence. All the animals examined were infected with nematodes A. sidemi with an exception of one bison, that was shot in year 2003. There was much higher average intensity of invasion in bison (5529), than in red deer (85) and in roe deer (1837). The animals were shot in a period from December to March, and the nematodes found in them were fourth stage larvae and immature adult specimens. In the following years of examinations, a gradual increase in average intensity of infection was observed. In 2007, it reached in bison 10814 nematodes. Maximum intensity of invasion was found in this year as well and it reached 44310 nematodes in one bison. Pathological changes such as an oedema, hyperaemia and effusion in the abomasum and duodenum mucosa were most clearly seen in the calves that were highly infected. These changes probably lead to chronic diarrhoea, deterioration and deaths of young animals.
The aim of this work was to examine if the game species from the north-western Poland, roe deer (Capreolus capreolus), red deer (Cervus elaphus) and wild boar (Sus scrofa), may be reservoir hosts of bacteria from the genus Bartonella, and whether the sheep tick (Ixodes ricinus) is their vector. To this end, the prevalence of Bartonella DNA in the tissues of these game species was measured, just as in sheep ticks (I. ricinus) infesting them, and ticks collected from plants in the hunting area. The prevalence of Bartonella DNA was 39% (23/59) in roe deer and 35% (7/20) in red deer. No Bartonella DNA was detected in any of the 21 wild boars. The presence of Bartonella DNAwas detected in 1.9% of ticks infesting roe deer (2/103), while no pathogen DNA was found in the 20 ticks infesting the red deer and the 3 ticks infesting wild boars, or the 200 ticks collected from plants. Amplicons of two different lengths were obtained; 198 bp, characteristic for B. bovis, and 317 bp, characteristic for B. schoenbuchensis, which were confirmed later by sequencing. The examined ruminants are probably the reservoir hosts of B. schoenbuchensis and B. bovis in the biotope of the Puszcza Wkrzańska Forest, and wild boars do not participate in the Bartonella propagation in the environment. I. ricinus is unlikely to be the main vector of Bartonella species detected in the examined roe deer and red deer; probably other bloodsucking arthropods, parasitizing wild ruminants, play this role.
We tested the reliability of fecal nitrogen (FN) to predict dietary nitrogen (DN) in two sika deerCervus nippon Heude, 1884 populations with greatly differing habitats. One was near the village of Nishiokoppe (area A) and the other was on Nakanoshima Island (area B) in Hokkaido, Japan. To estimate FN, we washed feces through a sieve, and diet was identified based on rumen-content analysis. The diet in area A was mostly composed of grass and legumes of agricultural origin, with browse being only a minor component. In contrast, browse such as deciduous foliage was the main component of the diet in area B. Dietary nitrogen was significantly regressed by FN within specific areas. On the other hand, the DN-FN-relationship had a similar slope but significantly different intercepts between areas. DN-FN-relationships differed between diets with and without an agricultural component, irrespective of browse. Thus, the difference in the DN-FN-relationship between areas was explained by differences in dietary composition. We therefore conclude that FN may be useful in predicting DN in diverse dietary situations regardless of the ratio of browse in the diet of free-ranging ungulates, but that dependence on agricultural pastures may hinder the utility of FN as an index of DN.
The aim of the study was to evaluate the stomach chambers of Zielonogorskie (Z) and Bieszczady (B) red deer (Cervus elaphus L. 1758) from different foraging habitats in South-West and South-East Poland. Thirteen calves, ten hinds and bulls were shot in Z and B, and examination of the carcasses indicated the substantial impact of foraging strategies in various habitats on polygastric parameters. Differences (P<0.05) in stomach content (SC), fresh and dry matter of stomach tissue (FSTM, DSTM) and total stomach area (TSA) were noted between the deer of Z and B. Additionally, some indexes (IA, IB) of FSTM, DSTM in relation to body mass (carcass weight, C) were relatively higher in each category of deer from the B region. Comparisons of ID (ratio DSTM to TSA), IE (DSTM to metabolic body mass - C0.75) and IF (TSA to C0.75 ) indicated a relatively thicker stomach tissue wall, and its larger overall area and volume of some stomach chambers in deer from the B vs. Z region. The study indicates that the carrying capacity of habitats, and conditions such as the quality and abundance of plants (their structure and nutritional value) considerably influence modifications within the digestive system, and mainly the stomach chambers in polygastric wild ruminants.
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