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Activity of beta-fructofuranosidase from the bird cherry leaves purified with the use of column chromatography

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Ciepiela A. P., Sytykiewicz H., Chrzanowski G., Czerniewicz P.

Herba Polonica

2004

tom 50

nr 3-4

The aim of the study was to determine the activity of ß-fructofuranosidase isolated from the bird cherry leaves collected in the period of spring development (April-May). The extracted enzyme was subjected to preliminary purification with ammonium sulphate and further purification with the use of column chromatography (Sephadex G-150). The obtained results proved that extracts purified by means of chromatography contained a more active enzyme and lower protein content in comparison with the crude ones. Furthermore, a statistically significant gradual decrease in invertase activity in the bird cherry leaves was noticed during the analysed period of vegetation.

Ograniczanie wyników

1 Herba Polonica

1 Chrzanowski G.

1 Ciepiela A. P.

1 Czerniewicz P.

1 Sytykiewicz H.

1 2004

Wyniki wyszukiwania

Activity of beta-fructofuranosidase from the bird cherry leaves purified with the use of column chromatography