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This paper presents the influence of penicillin, erythromycin, Oxytetracycline and streptomycin on aquatic microorganisms isolated from three reservoirs with varied extent of environmental pollution (Sulejów Reservoir, Zegrze Reservoir and Vistula River). The experiments were conducted in especially prepared microcosms (aerated 15 l aquariums). From among the examined antibiotics streptomycin showed the longest activity in the water environment (27 days), followed by Oxytetracycline (22 days). Erythromycin had 13 days activity while the penicillin only 4 days. The highest number of bacteria was observed in the Vistula, fewer in Zegrze Reservoir. Sulejów Reservoir was characterized by the lowest number of bacteria. The strains isolated from Sulejów Reservoir were characterized by higher degree of biodiversity than those from Zegrze Reservoir or Vistula River. The dominant genera were Pseudomonas (49%), followed by Acinetohacter (21 %), Flavomonas (9%), Alcaligenes (9%), Vibrio, Moraxella and Oligella. The Vistula River was dominated by Aeromonas (88%) and Pseudomonas (12%) genera. The strains isolated from Sulejów Reservoir were more sensitive to antibiotics than those from the much more polluted Vistula, this being evidenced by lower MIC and MBC values.
The occurrence and growth of aquatic zoosporic microorganisms on the tick Dermacentor reticulatus F. were investigated in laboratory conditions. Water samples were collected from sites of the Narewka River and natural ponds in Białowieża National Park. Some physico-chemical parameters were analyzed as well. The highest contents of ammonium nitrogen and phosphates were found in the water of the Narewka River. Forty-six species of zoosporic microorganisms were isolated from Dermacentor reticulatus F. ticks, including 8 of the Chytridiomycetes class, 1 of Plasmodiophoromycetes, 35 of Peronosporomycetes and 2 Zygomycetes. The highest number of species was identified in ponds situated in the village of Białowieża (30), the lowest (22) in the Narewka River outside the village. The most common aquatic Peronosporomycetes included Achlya americana, Ac. prolifera, Aphanomyces laevis, Ap. irregularis, Dictyuchus monosporus and Saprolegnia ferax, found on the fragments of most ticks in the river and pond water. Cluster analysis of the investigated parameters revealed that the environmental factors determining the number of aquatic zoosporic microorganisms was oxidability in the Narewka River, and nitrate and nitrite in the ponds of Białowieża village.
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