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1

Paramphistome species in water buffaloes and intermediate hosts in the Kizilirmak Delta in Samsun Province, Turkey

100%
Ates C., Umur S.
Acta Parasitologica
|
2021
|
tom 66
|
nr 1
2

First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt

100%
Morsy K., Abdel-Ghaffar F., Dajem S. B., Abdel-Gaber R., El Gazar F.
Acta Parasitologica
|
2018
|
tom 63
|
nr 2
3

Biology of Bubalus bubalis

100%
Czerniawska-Piatkowska E., Chocilowicz E., Szewczuk M.
Annals of Animal Science
|
2010
|
tom 10
|
nr 2
107-115
4

PCR detection of Neospora caninum in water buffalo foetal tissues

100%
Auriemma C., Lucibelli M. G., Borriello G., De Carlo E., Martucciello A., Schiavo L., Gallo A., Bove F., Corrado F., Girardi S., Amoroso M. G., Uberti B. D., Galiero G.
Acta Parasitologica
|
2014
|
tom 59
|
nr 1
The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.
5
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Evidence of Fasciola spp. resistance to albendazole, triclabendazole and bromofenofos in water buffaloes (Bubalus bubalis)

84%
Venturina V. M., Alejandro A. F., Baltazar C. P., Abes N. S., Mingala C. N.
Annals of Parasitology
|
2015
|
tom 61
|
nr 4
Fasciolosis caused by Fasciola spp. is considered the most important helminth infection of ruminants in tropical countries. Anthelmintic resistance has become a global concern. This study compared the efficacy of the commonly used anthelmintics, determined the toxicity level and any indication of resistance. Thirty two water buffaloes naturally-infected with Fasciola spp. were used to determine the efficacy of triclabendazole (TBZ), albendazole (ABZ), and bromofenofos (BRO) using Fecal Egg Count Reduction Test (FECRT). To test the toxicity of the drugs given, serum glutamic-pyruvic transaminase (SGPT) was evaluated before and within one week after treatment. One dose administration of ABZ registered an efficacy of 79.17%, 73.33% for TBZ and 70.83% for BRO. Efficacy in two dosetreatment group was 83.33% for both BRO and ABZ, and 90.00% for TBZ. Two dose-treatment was effective for TBZ (90%), ineffective for BRO and ABZ. SGPT levels were not significantly different between pre-treatment and posttreatment across all treatments. Giving one or two doses of anthelmintics, at one month interval, does not increase the efficacy of the three drugs tested. The study also implies that anthelmintic resistance may have developed in the animals.
6
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A comparison of FLOTAC and CFF techniques in detecting gastrointestinal parasites in water buffaloes (Bubalus bubalis)

84%
Salvador R. T., Abalos R. P., Ruba A. M., Mingala C. N.
Annals of Parasitology
|
2014
|
tom 60
|
nr 2
The objective of the study was to compare the usefulness of FLOTAC and centrifugal fecal flotation (CFF) techniques. More specifically, the taxonomic classes (Nematoda and Cestoda) of endoparasites present in fecal samples of buffaloes are identified, the sensitivity and specificity of FLOTAC relative to CFF are calculated, and the agreement of both techniques is evaluated using Kappa statistics. Fresh fecal samples from 220 buffaloes in 10 municipalities were collected. Sheather’s sugar was used as a flotation solution for both the FLOTAC and CFF techniques. Of the 220 animals, 109 samples were nematode positive and 111 samples were nematode negative according to the FLOTAC technique, while 74 were found to be positive and 146 negative according to the CFF technique. No cestodes were detected by either technique. The calculated sensitivity for FLOTAC is 89.19% and its specificity is 70.55%. Kappa statistics revealed moderate agreement (k=0.535) between the two techniques in detecting nematodes. The prevalence observed based on FLOTAC and CFF test were 49.54% (109/220; 95% CI: 47.75–56.34) and 33.64% (72/220; 95% CI: 27.42–40.3), respectively.
7

Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

84%
Villareal M. V., Mingala C. N., Rivera W. L.
Acta Parasitologica
|
2013
|
tom 58
|
nr 1
Trypanosoma evansi infection in the Philippines is frequently reported to affect the country’s livestock, particularly, the buffaloes. To assess the prevalence and intraspecific diversity of T. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. T. evansi was detected in all 79 animals tested using PCR targeting the RoTat 1.2 VSG gene. Sequencing of the rDNA complete internal transcribed spacer (ITS) region including the 5.8S subunit showed high similarity (99–100%) between Philippine isolates and known T. evansi isolates in Genbank. Tree construction based on the same region confirmed the close relationship between Philippine and reported Thai isolates as compared to Egyptian isolates separated by relatively small genetic distances, 47 polymorphisms, despite the clustering in four branches. Overall, the results of this study prove genetic diversity within T. evansi species despite previous reports on limited heterogeneity among isolates worldwide.
8

Prevalence and first molecular identification of Sarcocystis species in cattle and water buffaloes in India

84%
Daptardar M., Singh B. B., Aulakh R. S., Gill J. P. S.
Acta Parasitologica
|
2016
|
tom 61
|
nr 3
9

Occurrence of Neospora caninun antibodies in water buffaloes [Bubalus bubalis] from the South-Western region of Iran

84%
Hajikolaei M. R. H., Goraninejad S., Hamidinejat H., Ghorbanpour M., Paryab R.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
To investigate the prevalence of Neospora canimim antibodies in water buffalo (Bubalus bubalis) in the north-western region of Iran, blood samples were taken from 181 (141 females and 40 males) buffaloes slaughtered in an abattoir in Ahvaz. Sera were tested for the presence of antibodies to N. canimim by an ELISA kit. The overall prevalence of Neospora canimim infection in the buffaloes tested was 37% (43.36% in females and 15% in males). Statistical analysis showed significant differences between male and female buffaloes. In female buffaloes, 7.7% of heifers and 56.8% of cows were seropositive. Statistical analysis showed the differences among female buffaloes were highly significant when their age is considered, and buffalo cows showed higher N. caninum prevalence when compared with heifers. With regard to the results of this study, high exposure of water buffaloes to N. canimim, and economical importance of buffalo industry to some regions of Iran, the role of N. canimim as a causal agent of abortions in this species should be investigated.
10

Evaluation of an ELISA for the diagnosis of sarcocystosis in water buffaloes

84%
Ghorbanpoor M., Hamidinejat H., Nabavi L., Khadjeh G. H., Jalali M. R.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
To investigate the occurrence of sarcocystosis in water buffalo (Bubalus bubalis) in Ahvaz, the Khuzestan province, Iran, and to evaluate an ELISA for the diagnosis of sarcocystosis, serum and oesophagus samples were collected from the 300 water buffaloes, aged 0.5-7 years, and then slaughtered at the Ahvaz abattoir. The oesophagus samples were examined for sarcocysts under microscopic examination, using the digestion method. One hundred and seventy-one (57%) animals were found to be positive for Sarcocystis bradyzoites. One hundred and sixty-three (54.3%) serum samples were positive for sarcocystis antibodies in the ELISA. The prevalence of sarcocystosis was statistically age related, with significantly higher rates in adult buffaloes than young animals (P<0.05). The prevalence did not differ significantly in relation to the gender (P>0.05). The Mc Nemar test revealed a high correlation (94%) between the digestion method and ELISA. The ELISA, with the use of antigens from S. fusiformis bradyzoites, as presented in this study, can be adapted to detect antibodies to Sarcocystis sp., with an acceptable specificity and sensitivity.
11

Caudal extradural analgesia with lidocaine, xylazine, and a combination of lidocaine and xylazine in the Iranian river buffalo

84%
Saifzadeh S., Pourjafar M., Naghadeh B. D., Jalali F. S. S.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
This study was conducted to compare the time of onset, duration of action, and the extent of analgesia produced by a lidocaine/xylazine combination with those produced by lidocaine and xylazine alone after injection into the caudal extradural space of the Iranian river buffalo. The study was designed as a prospective, descriptive, observer-blind trial, in a Latin square pattern. Eleven adult (aged over 2 years) non- gravid and healthy females of Iranian river buffaloes (Bubalus bubalis), weighing from 450 to 650 kg, were used. Caudal extradural analgesia was achieved on 3 occasions at 14 d intervals by injection of 2% lidocaine (L; 0.22 mg kg⁻¹), 2% xylazine (X; 0.05 mg kg⁻¹), and a combination of 2% lidocaine(0.22 mg kg⁻¹) / 2% xylazine (LX; 0.05 mg kg⁻¹) in a Latin square design. Analgesia was determined by the lack of response to pinprick and haemostat pressure in the skin of the caudal areas. X was significantly longer (5.5 ± 0.7 min) than that by L or LX. Duration of analgesia was significantly longer by LX (172.3 ± 17.7 min) than that by either drug used alone (lidocaine, 79.5 ± 5.7 min; xylazine, 136.4±11.4 min). In X and LX groups, the level of analgesia ascended to thoracic segments; however, in lidocaine-treated buffaloes thighs, flank, and udders remained sensitive. In all the buffaloes, xylazine, administered either alone or with lidocaine, induced mild to moderate ataxia. It was concluded that the LX combination provided a more rapid onset and a longer duration of analgesia, and a more cranial spread of analgesic effect compared with either drug alone. As a result, the LX combination may offer a fast and long lasting anaesthesia/analgesia to perform obstetrical and surgical procedures without the need for re-injection.
12

Occurrence of antibodies to Toxoplasma gondii in water buffaloes and meat cattle in Rio Grande do Sul State, Southern Brazil

67%
de Santos L. M. J. F., Dame M. C. F., Cadematori B. G., Filho N. A. C., Farias N. A. R., Ruas J. L.
Acta Parasitologica
|
2013
|
tom 58
|
nr 3
Serum samples from 169 water buffaloes and 121 beef cattle were analyzed for antibodies to T. gondii by an indirect fluorescent antibody test (IFAT). Positive results were obtained in 27.2% of water buffaloes and 17.4% of cattle. Statistical analysis indicated significant differences between the prevalence in cattle and buffalo (p ≤ 0.05). The highest titres found in positive animals were 1:256 (buffaloes) and 1:64 (cattle). In both bovine species, toxoplasmosis frequency in young animals (less than 2 years old) was lower compared to older individuals, although the differences seen in cattle were not statistically significant.
13

Serum selenium concentrations in water buffaloes (Bubalus bubalis) and black-and-white German Holstein-Friesian cows raised in the Notec River region of the Wielkopolska province

67%
Sablik P., Kobak P., Pilarczyk B., Szarkowski K., Syczewski A.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
Concentration of selenium in serum samples was determined using a modified Watkinson's spectrofluorometric method. Selenium concentration averaged 0.021 ±0.008 µg/mL for water buffaloes and 0.074 ±0.017 µg/mL for dairy cows. The analysis of selenium concentrations in cows showed deficiency level in 2.0%, marginal level in 64%, and optimal level in 34% of the examined samples. In water buffaloes, deficiency level was found in 96.55% and marginal level in 3.45% of the animals. Selenium levels in cattle and water buffaloes should be periodically diagnosed to provide data for veterinarians to make appropriate decisions. The implementation of suitable prophylactic programmes will enable optimum levels of this element to be maintained.
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