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Secretion of stress-related proteins by suspension-cultured Lupinus albus cells

100%

Wojtaszek P., Pislewska M., Bolwell G. P., Stobiecki M.

Acta Biochimica Polonica

1998

tom 45

nr 1

A suspension culture of white lupin cells has been established, and proteins of the exocellular matrix analysed. Based on homologies of N-terminal amino-acid sequences, three stress- or defence-related proteins: acidic class III chitinase, polygalacturonase-inhibiting protein, and germinóxalate oxidase, secreted by lupin cell culture, were identified.

Genotyping of Cryptosporidium parvum isolates obtained from humans in South Africa

80%

Paulsen L., Venter S. N.

Acta Parasitologica

2003

tom 48

nr 2

Cryptosporidium parvum is endemic to South Africa. In order to provide direction for prevention strategies, information on the dominant genotype involved in human infections is required. A combination of diethyl ether extraction, sodium chloride flotation and immuno-magnetic separation (IMS) was used for maximum recovery of oocysts from faecal samples. In order to improve the sensitivity of PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene, a nested PCR was developed. RFLP analysis was done on a 553 bp segment obtained from the final nested PCR reaction to distinguish between C. parvum genotype 1 (human exclusive type) and genotype 2 (broad host range). Overall, genotype 2 was detected in 10 samples, whereas genotype 1 was detected in the remaining 40 samples. The high incidence of genotype 1 parasites (80%) implies hat direct and indirect human transmission plays a dominant role in the epidemiology of the disease caused by this parasite in the study area.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Parasitologica

1 Bolwell G. P.

1 Paulsen L.

1 Pislewska M.

1 Stobiecki M.

1 Venter S. N.

1 Wojtaszek P.

1 2003

1 1998

Wyniki wyszukiwania

Secretion of stress-related proteins by suspension-cultured Lupinus albus cells

Genotyping of Cryptosporidium parvum isolates obtained from humans in South Africa