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1

Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids

100%
Suluja E., Strokowskaja L., Zagorski-Ostoja W., Palucha A.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 3
Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.
2

Virus-like particles as vaccine

75%
Chroboczek J., Szurgot I., Szolajska E.
Acta Biochimica Polonica
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2014
|
tom 61
|
nr 3
3

Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

63%
Huang D., Chao Y.-C., Lv Z., Jan J.-T., Yang Y.-C., Hsiao P.-W., Wu C.-Y., Liao C.-H., Wu T.-H., Wang Y.-C.
Journal of Veterinary Research
|
2021
|
tom 65
|
nr 2
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Absence of evidence for viral infection in colony-embedded cyanobacterial isolates from the Curonian Lagoon

63%
Sulcius S., Staniulis J., Paskauskas R., Olenina I., Salyte A., Ivanauskaite A., Griniene E.
Oceanologia
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2014
|
tom 56
|
nr 3
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Expression of hepatitis B core antigen in lettuce and tobacco for advanced plant-derived vaccine formulations

63%
Pyrski M., Kasprzyk A., Bogusiewicz M., Bociag P., Czyz M., Pniewski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
|
nr 1
6

Plant expression systems for production of hemagglutinin as a vaccine against influenza virus

63%
Redkiewicz P., Sirko A., Kamel K. A., Gora-Sochacka A.
Acta Biochimica Polonica
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2014
|
tom 61
|
nr 3
7

Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus

63%
Gromadzka B., Szewczyk B., Konopa G., Fitzner A., Kesy A.
Acta Biochimica Polonica
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2006
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tom 53
|
nr 2
Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5'-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.
8

Usefulness and efficiency of formalin-fixed paraffin-embedded specimens from laryngeal squamous cell carcinoma in HPV detection by IHC and PCR/DEIA

51%
Morshed K., Polz-Dacewicz M., Szymanski M., Smolen A.
Folia Histochemica et Cytobiologica
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2010
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tom 48
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nr 3
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Morphology and distribution of phage-like particles in a eutrophic boreal lagoon

51%
Sulcius S., Staniulis J., Paskauskas R.
Oceanologia
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2011
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tom 53
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nr 2
In this paper we present the results of direct observations of the morphology and size of phage-like particles by means of transmission electron microscopy (TEM) as a function of their spatial distribution in the shallow highly productive Curonian Lagoon of the Baltic Sea. In total, 26 morphologically different forms of phagelike particles were found. Different trends of distribution in terms of abundance, size and shape of virus-like particles were demonstrated. The total abundance of viruses varied from 1.91×107 ml−1 to 5.06×107 ml−1. The virus to bacteria ratio (VBR) changed from 15.6 to 49 and was negatively associated with total bacterial numbers (r = −0.60; p < 0.05). The phages of family Myoviridae were the most diverse and were dominant at all stations.
10

Nanogram doses of alum-adjuvanted HBs antigen induce humoral immune response in mice when orally administered

51%
Kapusta J., Pniewski T., Wojciechowicz J., Bociag P., Plucienniczak A.
Archivum Immunologiae et Therapiae Experimentalis
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2010
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tom 58
|
nr 2
143-151
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