Spontaneous infection of horses with Equine Arteritis Virus can occur either through the respiratory or the reproductive system. In case of infection through the reproductive system, the semen of the convalescent stallion is the source of virus and the infection takes place during natural mating or artificial insemination. The main purpose of serological and virological investigations thus is to detect stallions infected with Equine Arteritis Virus being virus shedders from semen. In this study, samples of sera collected from horses kept in natural conditions were examined. They were investigated with virus neutralisation test for anti Equine Arteritis Virus antibodies using various Equine Arteritis Virus strains as antigens. In sera of the horses examined, high anti Equine Arteritis Virus antibody titers from modified Bucyrus Equine Arteritis Virus strain, passage 166 in RK-13 cells and from Wroclaw Equine Arteritis Virus, passage 20 in RK-13 cell line were shown. Although there were considerable differences in the level of anti Equine Arteritis Virus antibodies for the antigens used, serum samples identified in the standard virus neutralisation test as free from anti Equine Arteritis Virus antibodies remained negative for all Equine Arteritis virus strains used in the experiment.
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