Wyniki wyszukiwania

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Co-inoculation with two non-infectious cDNA copies of potato spindle tuber viroid [PSTVd] leads to the appearance of novel fully infectious variants

100%

Podstolski W., Gora-Sochacka A., Zagorski W.

Acta Biochimica Polonica

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2005

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tom 52

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nr 1

Potato spindle tuber viroid (PSTVd) is one of the smallest (about 360 nt) infectious plant agents. It is composed of a single-stranded circular non-coding RNA molecule. In the course of previous passage experiments with two intermediate PSTVd variants I2 and I4, three non-infectious clones (I2-50, I4-37 and I4 VI-17) were found. When inoculated separately as cDNAs on tomato “Rutgers” test plants these variants did not induce any visible disease symptoms and did not produce progeny. The presence of such non-infectious variants raises several questions about their origin and biology and to answer them, mixed co-infections with cDNA copies of two non-infectious variants (I2-50, I4-37) were performed. PSTVd infection was observed in seven out of 30 inoculated plants. The progeny isolated from three separate plants contained novel variants, together with the parental I2 and I4 sequences. It is conceivable that the appearance of repaired PSTVd molecules, clearly capable of cell-to-cell movement leading to the systemic infection, results from recombination events. An analysis of the recombinant molecules and comparison with databases identified the specific sites responsible for the restricted infectivity of the I2-50 and I4-37 PSTVd variants. In parallel experiments in which (+) strand PSTVd infectious transcripts were used, no recombinants were observed, and the original I2-50 and I4-37 non-infectious sequences were not detected in the progeny.

2

The reaction of field-grown tomato cultivars to infection with potato spindle tuber viroid [PSTV]

100%

Kryczynski S., Stawiszynska A., Abuhliga T. A.

Phytopathologia Polonica

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1995

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tom 10

3

Viroids: unusual small pathogenic RNAs

100%

Gora-Sochacka A.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

Viroids are small (about 300 nucleotides), single-stranded, circular, non-en- capsidated pathogenic RNA molecules. They do not code for proteins and thus depend on plant host enzymes for their replication and other functions. They induce plant diseases by direct interaction with host factors but the mechanism of pathogenicity is still unknown. They can alter the expression of selected plant genes important for growth and development. Viroids belong to two families, the Avsunviroidae and the Pospiviroidae. Viroids of the Avsunviroidae family adopt a branched or quasi rod-like secondary structure in their native state. Members of the Pospiviroidae family adopt a rod-like secondary structure. In such native structures five structural/functional domains have been identified: central (C), pathogenicity, variable and two terminal domains. The central conserved region (CCR) within the C domain characterizes viroids of the Pospiviroidae. Specific secondary structures of this region play an important role in viroid replication and processing. Viroids of the Avsunviroidae family lack a CCR but possess self-cleaving properties by forming hammerhead ribozyme structures; they accumulate and replicate in chloroplasts, whereas members of the Pospiviroidae family have a nuclear localization. Viroid replication occurs via a rolling circle mechanism using either a symmetric or asymmetric pathway in three steps, RNA transcription, processing and ligation.

4

Cytopathological changes in nucleus of Lycopersicon esculentum cult. Rutgers plants infected with potato spindle tuber viroid [PSTVd]

84%

Rudzinska-Langwald A.

Annals of Warsaw Agricultural University. Agriculture

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1999

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nr 35

3-12

5

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The use reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]

84%

Cajza M., Wypijewski K., Pospieszny H.

Journal of Plant Protection Research

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1997

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tom 37

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nr 1-2

The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors were good for reverse transcription and later for amplification of the HLVd-cDNA. The presence of gelatin as a stabilizer of DNA polymerase was indispensable for successful performance of RT-PCR.

6

Occurrence of the apple scar-skin viroids group in Polish orchards

84%

Paduch-Cichal E., Welnicki M., Skrzeczkowska S., Slowinski A.

Phytopathologia Polonica

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1996

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tom 11

7

Translocation and distribution of potato spindle tuber viroid in infected plants

84%

Kryczynski S., Stawiszynska A., Abuhliga T. A.

Phytopathologia Polonica

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1999

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tom 17

8

Translocation and distribution of Chrysanthemum stunt viroid in infected plants

84%

Kryczynski S., Stawiszynska A.

Phytopathologia Polonica

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1999

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tom 17

9

Zastosowanie metody dynamiki molekularnej w przestrzeni katow torsyjnych [TAD] jako proba wyznaczenia struktur trzeciorzedowych wiroida wrzecionowatosci bulw ziemniaka [PSTVd]

67%

Folkman W., Popenda M., Cajza M.

Progress in Plant Protection

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2006

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tom 46

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nr 2

588-591

10

Genetic variability of potato spindle tuber viroid RNA replicon

67%

Gora-Sochacka A., Candresse T., Zagorski W.

Acta Biochimica Polonica

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2001

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tom 48

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nr 2

The genetic con ti nu ity of the po tato spin dle tu ber viroid (PSTVd) ge nome was analysed af ter in fec tion of to mato plants with cloned cDNAs of pa ren tal strains. Dur ing the six weeks of the experiment, several new sequence variants appeared. The sequence vari ants de tected in the prog eny pop u la tion in duced se quence-specific dis ease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains prop a gat ing in plants as a pop u la tion of sim i lar se quences. As sessing fur ther the replicon con ti nu ity, a PSTVd cDNA mu tant with a de le tion in the cen tral con served re gion was con structed and proven to be non-infectious. Sur pris ingly, in a sub-population of po tato transformants ex press ing the same de leted PSTVd RNA an in fec tious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.

11

Wirusy i patogeny wirusopodobne roślin jagodowych utrzymywane w kolekcji Pracowni Wirusologii Instytutu Sadownictwa i Kwiaciarstwa

43%

Cieslinska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2009

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tom 539

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nr 1

W kolekcji wirusów, fitoplazm i wiroidów, utrzymywanej w Pracowni Wirusologii Instytutu Sadownictwa i Kwiaciarstwa w Skierniewicach, gromadzone są rośliny sadownicze i ozdobne porażone przez różne izolaty tych patogenów. Jedna z części kolekcji obejmuje rośliny jagodowe, w których na podstawie wyników przeprowadzonych testów biologicznych, serologicznych (ELISA) i/lub PCR wykryto następujące wirusy i fitoplazmy: truskawka: wirus cętkowanej plamistości liści truskawki, wirus marszczycy truskawki, ʻCandidatus Phytoplasma asteris’; malina: wirus chlorozy nerwów liści maliny, wirus krzaczastej karłowatości maliny, wirus plamistości liści maliny, ʻCandidatus Phytoplasma ulmi’; jeżyna: ʻCandidatus Phytoplasma ulmi’, ʻCandidatus Phytoplasma pini’; porzeczka czarna: wirus rewersji porzeczki czarnej; porzeczka czerwona: wirus otaśmienia nerwów agrestu, wirus mozaiki ogórka; agrest: wirus otaśmienia nerwów agrestu; borówka wysoka: ‘Candidatus Phytoplasma asteris’, mozaika borówki wysokiej.

Ograniczanie wyników

Co-inoculation with two non-infectious cDNA copies of potato spindle tuber viroid [PSTVd] leads to the appearance of novel fully infectious variants

The reaction of field-grown tomato cultivars to infection with potato spindle tuber viroid [PSTV]

Viroids: unusual small pathogenic RNAs

Cytopathological changes in nucleus of Lycopersicon esculentum cult. Rutgers plants infected with potato spindle tuber viroid [PSTVd]

The use reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]

Occurrence of the apple scar-skin viroids group in Polish orchards

Translocation and distribution of potato spindle tuber viroid in infected plants

Translocation and distribution of Chrysanthemum stunt viroid in infected plants

Zastosowanie metody dynamiki molekularnej w przestrzeni katow torsyjnych [TAD] jako proba wyznaczenia struktur trzeciorzedowych wiroida wrzecionowatosci bulw ziemniaka [PSTVd]

Genetic variability of potato spindle tuber viroid RNA replicon

Wirusy i patogeny wirusopodobne roślin jagodowych utrzymywane w kolekcji Pracowni Wirusologii Instytutu Sadownictwa i Kwiaciarstwa