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A vigour test that can predict the field emergence percentage of tree seeds accurately has long been a wish from growers. A new test method, the Critical Root Length (CRL) vigour test, was developed for beech seeds on the basis of the length of primary roots, germinated seeds can produce during a specified test. Pretreated, imbibed seeds were germinated in a vertically positioned moist paper roll during 20days at 15°C in 12 hour light daily. Root length of normally germinated seedlings was recorded and correlated with field emergence percentage to obtain a critical root length for the ability to emerge in the field. Critical root length for Fagus sylvatica was found to be 45 mm. The percentage of normally germinated seeds with roots longer than 45 mm in the CRL test is a predicted estimate of the field emergence percentage of a seed lot. Results of two tests on 5 and 10seed lots showed generally good correlation between CRL predicted emergence and actually obtained field seedling emergence. Large variation in root length was found between and within seed lots, thus displaying large differences in seed vigour. The new test is an applied, easy and inexpensive vigour test developed for nurserymen and seed technicians in order to predict field emergence more accurately.
Many seed lots of beech (Fagus sylvatica L.) nuts are characterized by various levels of infection with pathogenic fungi, such as Fusarium, Rhizoctonia and Cylindrocarpon spp. Favourable conditions for fungal infection of beechnuts are created especially during their long-term provisional storage without earlier drying to the moisture content of 8-10%. Strongly infected seed lots are not suitable for long-term storage. During the pre-sowing treatment of seeds by stratification without medium, fungi develop under the moist conditions and infect many healthy seeds. To assess the quality of beechnuts, a vigour test has been developed. Seeds removed from the pericarp (4 replications of 50 nuts each) are placed in boxes between two layers of moist tissue paper and incubated at 25°C for 120 hours. After this period decayed seeds (mouldy seeds) are excluded, and the remained are subjected to embryo viability by the tetrazolium test. When the percentage of decaying seeds (including those with necroses) does not exceed 10-15%, such seed lot can be used for long-term storage. If the percentage exceeds 50%, the seed lot should be considered unsuitable both for storage and for pre-sowing treatment, because only a very small proportion of seeds will emerge in the nursery.
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