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Three seed priming techniques: hydropriming, halopriming and osmopriming, were compared for their effects on germination and vigour of pansy (Viola × wittrockiana Gams.) seeds at 20°C, 30°C and 35°C. Seeds were hydroprimed in the restricted volumes of water (600 and 700 μl H2O·g seed-1, 2, 3 or 4 days), haloprimed in KNO3 solution (-1.5 MPa, 5 days), and osmoprimed in polyethylene glycol solutions (-1.0, -1.25 or -1.5 MPa PEG 8000, 7 days) at 15°C or 20°C. Seed germination and vigour tests were performed for untreated and primed seeds. Generally, hydropriming negatively affected the speed of germination, the percentage of germinating seeds and germination capacity. Halopriming accelerated seed germination at 20°C, 30°C and 35°C but did not influence the percentage of germinating seeds and germination capacity. Osmopriming of seeds in PEG solution of osmotic potential –1.0 MPa at 20°C not only improved germination rates at 20°C, 30°C, and 35°C to the highest extent, but also increased percentage of germinating seeds at 30°C and 35°C most effectively and positively affected seed germination capacity at 20°C and 30°C.
The experiment was conducted in the Experimental Station in Baranowo near Poznań between 2007-2009. The influence of mycorrhizal fungi on the growth and yield of the plum tree cultivar - ' Cacanska Lepotica' and sour cherry tree cultivar - 'Schattenmorelle' was estimated. Three years after planting, the plum trees had bigger vigour of growth, expressed by TCSA, in a combination with mycorrhizal fungi. Sour cherry trees did not differ in growth after the use of mycorrhizal fungi. A higher yield of fruits was harvested from plum and sour cherry trees inoculated with a mycorrhizal fungi. Productivity of mycorrhized trees, calculated according to the cross-sectional area of the trunk, was higher than in the control. The use of mycorrhizal fungi had no influence on fruit mass of the investigated species of fruit trees.
In many countries, the traditional method of growing shallots from bulbs is replaced by direct seeding in the field or by planting seedlings. The availability of shallot seed with high parameters of the sowing value is an important condition for growing shallot in larger areas. It is possible thanks to the progress in breeding and introduction into cultivation of new cultivars that produce seed stalks and seed. Therefore, the aim of the present study was to evaluate the sowing value of seed of the true-breeding cultivar ‘Toto’, when this seed is obtained by planting steckling bulbs (bulb-to-seed method) and by using the seed-to-seed method. This study, conducted in the period 2010–2012, was to evaluate some important characteristics of the seed quality obtained by planting different diameter bulbs (20–60 mm, 4 fractions every 10 mm) in autumn and spring and from seedlings planted in summer (third 10-day period of July and first and third 10-day period August). The quality of shallot seed was evaluated taking into account the following parameters: 1000 seed weight (TSW), germination energy and capacity as well as vigour tests (seedling growth test and seedling growth rate test). Shallot cultivation method had a large effect on 1000 seed weight, but it only slightly affected germination capacity and vigour of seed obtained. Larger seeds were obtained from bulbs, regardless of their planting time, compared to the cultivation method involving planting seedlings in summer. The diameter of bulbs used for planting at autumn planting time proved to be an important factor for TSW, germination energy and the traits determining seed vigour. In the case of this planting time, seed obtained from large bulbs with a diameter of 40–60 mm was distinguished by the best quality. The study did not show such a correlation for seed derived from spring bulb planting.
The aim of the study was to examine response of ‘Jersey’ highbush blueberry to boron (B) fertilization. The experiment was carried out in 2002-2003 at a private plantation in Central Poland on mature blueberries planted on a sandy loam soil with pH 4.1, low organic matter status, and medium available B content. Blueberries were supplied with B by foliar applications or broadcasting. Foliar B sprays were done in the spring or the fall. Spring B sprays were performed at the beginning of blooming, at petal fall, and 3 and 6 weeks after the ending of flowering. In each spring spray treatment, B was used at a rate of 0.2 kg ha⁻¹. Fall B spray was done 5 weeks after fruit harvest using 0.8 kg B ha⁻¹. Soil B was applied at the stage of bud break at a rate of 2 kg ha⁻¹. Blueberries unfertilized with B served as control. The results showed that soil B application and spring B sprays increased B status in flowers and leaves. However, B fertilization, regardless of application mode, had no effect on plant vigor, the number of flowers per cane, fruit set, mean fruit weight, berry firmness, and yielding. Berries of blueberries sprayed with B in the spring had increased soluble solids concentrations. It is concluded that: (i) B fertilizer requirements of ‘Jersey’ highbush blueberry are low, and (ii) at hot water extractable B concentration in a soil below 0.37 mg kg⁻¹ and/or at B status in leaves of current season shoots below 37 mg kg⁻¹, spring B sprays should be applied in blueberry culture to increase soluble solids concentration in fruit.
The nuclear replication stages in dry and germinating seeds of two sugar-beet cultivars were determined by flow cytometry. For each cultivar two seed lots of similar, high germination capacity, but different in seedling length vigour index (SLVI; applied as quality marker) were studied. Analyses were performed every 12 hours during 72 hours of germination and early seedling growth at 20oC and 15oC. Before start of germination, in all seed lots, about 90% of cells were arrested in the G1 phase of the cell cycle. The proportion of G1 cells decreased with time of germination, faster in higher quality seed lots than in those of lower quality. The G2/G1 ratio in dry seeds was slightly higher for the seeds of higher SLVI, and it increased during germination in all seed lots. However, the difference between higher and lower quality seeds of the same cultivar did not become more distinct during germination.
The effect of presowing hydration treatment on DNA replication activity in the embryo of three sugar beet cultivars was studied using flow cytometry. The treatment protocol consisted of soaking the seeds for 2 hours in tap water and then for 2 hours in 0.2% NaOH solution. After drying, a slurry treatment with Oxafun T was applied. The applied treatment was aimed at removing soluble inhibitors from the pericarp and to control of damping off. Seedling length vigour index (SLVI), germination capacity and the relative proportions of the DNA replication stages were determined in untreated and treated seeds. Hydration treatment increased seed performance, in particular the vigour index. A higher G2/G1 ratio was noted for treated seeds than the untreated ones, which suggests that cells of the former seeds had entered the synthetic phase of the cell cycle. A positive correlation was found between the G2/G1 ratio and laboratory test parameters (SLVI and germination capacity).
The present study examines the mechanism of natural and accelerated ageing processes in Norway spruce (Picea abies [L.] Karst.) seeds stored at low temperature for one (control) and eight years. The analyses of vitality, electrolyte leakage, protein composition and activity of the proton-pump ATPase (P-ATPase, EC.3.6.1.35) in plasma membrane of Norway spruce seeds have been investigated. Seeds collected in 1999 from northeastern Poland have been treated with accelerated ageing method and compared to the seeds of the same provenance, collected in 1992 (control). The vigor and the vitality of the Norway spruce seeds depend on the age of seeds. After 6 days of experiment both samples of seeds (artificially aged seeds and control) expressed the same percentage of germination and similar electrolyte leakage. Analysis of protein composition in the extracts showed an increased amount of some low molecular-weight proteins in artificially and naturally aged seeds compared to the control. Our results indicate similar level of P-ATPase activity in natural, artificially aged and control seeds. This suggests that natural ageing process can involve changes in plasma membrane protein composition but does not affect the P-ATPase activity.
The electrical conductivity values for winter triticale seeds with sprouting damage, harvested in three successive years after different time of storage were compared with vigour indices: germination ability, length of shoots and roots. The percentage of variance components for all sources of variation and the coefficients of correlation between vigour indices were estimated and discussed.
The experiments were carried out in 2005-2007. The aim of the study was the evaluation of the growth and yielding of sour cherry cultivars in central Poland. The explored cultivars differed in vigour, cropping and also in response to spring frost. The most vigorous, indicated by the trunk cross-sectional area (TCSA), length of one­-year shoots and leaf area, were 'Sabina', 'Lucyna', 'Karneol' and 'Ujfehertoi Furtos'. The weakest in vigour were 'Northstar', 'Schattenmorelle IR-2' and 'Koral'. The best fruit set was observed in 'Schattenmorelle IR-2' and the worst in 'Karneol'. The cultivars 'Lucyna', 'Schattenmorelle IR-2', 'Sabina' and 'Vowi' gave the highest yield per tree. 'Karneol', 'Ujfehertoi Furtos', 'Northstar' and 'Debreceni Botermo' gave the lowest yield per tree. The greatest damage by the spring frosts of 2007 was observed in the following cultivars: 'Lucyna', 'Debreceni Botermo', 'Karneol' and 'Sabina'. The most resistant to spring frosts turned out to be 'Northstar' and 'Schat­tenmorelle IR-2'.
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