An inhibition of growth of Chlorella vulgaris, Beijerinck 1980 laboratory cultures carried out under different conditions caused by benzene, phenol and catechol was investigated. Each of the investigated substances was added to the culture medium at 4 or 5 different concentrations, directly before inoculation. The cultures were carried up to the stationary phase of growth. The density of alga cell suspension in the culture medium was determined with the use of a Bürker hemocytometer as well as EC50/96 and EC 50/Tmax values. It was stated that under some conditions it was possible to estimate the toxicity of the investigated substances, either in open cultures (flasks with microbiological corks, type I cultures) or closed (flasks with ground corks, type II cultures), but in this second case with the presence of NaHCO3 as an additional source of C02. The results presented in this paper indicate a relatively high toxicity of benzene and its investigated metabolites, phenol and catechol, as well as its dependence on conditions in the investigated cultures.
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