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The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.
Classically, the acid-base balance (ABB) is described by the Henderson-Hasselbach equation, where the blood pH is a result of a metabolic components – the HCO₃⁻ concentration and a respiratory component – pCO₂. The Stewart model assumes that the proper understanding of the organisms ABB is based on an analysis of: pCO₂, Strong Ion difference (SID) – the difference strong cation and anion concentrations in the blood serum, and the Acid total (Atot) – the total concentration of nonvolatile weak acids. Right sided heart failure in dogs causes serious haemodynamic disorders in the form of peripheral stasis leading to formation of transudates in body cavities, which in turn causes ABB respiratory and metabolic disorders. The study was aimed at analysing the ABB parameters with the use of the classic method and the Stewart model in dogs with the right sided heart failure and a comparison of both methods for the purpose of their diagnostic and therapeutic utility. The study was conducted on 10 dogs with diagnosed right sided heart failure. Arterial and venous blood was drawn from the animals. Analysis of pH, pCO₂ and HCO₃⁻ was performed from samples of arterial blood. Concentrations of Na+, K+, Cl-, Pinorganic, albumins and lactate were determined from venous blood samples and values of Strong Ion difference of Na+, K+ and Cl- (SID₃), Strong Ion difference of Na+, K+, Cl- and lactate (SID4), Atot, Strong Ion difference effective (SIDe) and Strong Ion Gap (SIG₄) were calculated. The conclusions are as follows: 1) diagnosis of ABB disorders on the basis of the Stewart model showed metabolic alkalosis in all dogs examined, 2) in cases of circulatory system diseases, methodology based on the Stewart model should be applied for ABB disorder diagnosis, 3) if a diagnosis of ABB disorders is necessary, determination of pH, pCO₂ and HCO₃⁻ as well as concentrations of albumins and Pinorganic should be determined on a routine basis, 4) for ABB disorder diagnosis, the classic model should be used only when the concentrations of albumins and Pinorganic are normal.
Due to the unique taste features and pro-health values of fruits, hot pepper is cultivated in Poland on the commercial scale, most frequently with the use of single harvest which results in fruits at various maturity stage: green fruits, fruits turning colour and physiologically mature fruits (red). This paper, based on the results of 3-year experiments, evaluates utility and biological value of fruits of four Polish cvs.: ‘Bronowicka Ostra’, ‘Orkan’, ‘Cyklon’ and ‘Rokita’. Agricultural study was conducted for three years, during the period 2009–2011. Fruits of various maturity stage consisted of the marketable yield of the and hot pepper from a single harvest. The evaluated cultivars differed in utility and biological value of fruits, quantity and fruit yield structure. The greatest yield was obtained from ‘Orkan’ and ‘Rokita’ cvs. (2.17 kg·m-2 ), yet the greatest yield of physiologically mature fruits (1.16 kg·m-2) from ‘Bronowicka Ostra’ cv. whose share in marketable yield was 65.0%. Biological value of fruits depended on maturity stage and cultivar genotype. Physiologically mature fruits contained most dry mass (13.18%), soluble solids (4.4%), L-ascorbic acid (199.9 mg·100 g-1 f.m.), total sugars (2.92%). Yet more capsaicinoids were proved in green fruits and fruits turning colour. Least dry mass (%) and total sugars (%) were noted in ‘Orkan’ cv., whereas least L-ascorbic acid in ‘Bronowicka Ostra’ cv., in which fruits at every stage of maturity accumulated most capsaicinoids.
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