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Plasminogen activator inhibitor type-1 controls the process of the in vitro sprout formation

100%

Chorostowska-Wynimko J., Skrzypczak-Jankun E., Jankun J.

Journal of Physiology and Pharmacology. Supplement

2004

tom 55

nr 3

49-56

Plasminogen activator inhibitor type-1 (PAI-1), the primary regulator of plasminogen activator - urokinase (uPA) plays a crucial role in the cell adhesion and migration and in angiogenesis. We had previously demonstrated that PAI-1 - endothelial cell interplay is critical for the formation of new blood vessels and the process is mostly conducted via uPA- anti-proteinase interaction. In the present study we wished to further examine the role of PAI-1 in the sprout formation, representing the first step of new capillary vessels development by evaluating the effect of PAI-1 on the sprout area. We addressed the issue by assessing the influence of cysteine-mutated PAI-1 proteins characterized by a prolonged half-life time (hDßT - T1/2= 63.59 h and ßT- T1/2= 6931.47 h), and therefore more stable anti-uPA activity, on the appearance of newly formed sprouts. We found that both CysPAI-1 proteins significantly diminished the mean sprout area in a concentration-dependent fashion. The inhibitory effect present in the two examined endothelial cells systems of different origin and functional characteristics - human umbilical vein endothelial cells (HUVEC) and human lung microvascular endothelial cells (HLMVEC) cultures - was noticeably greater for HLMVEC -high urokinase-producers. Moreover, the inhibition rate was significantly greater for the ßT mutant than that for the hDßT PAI-1 mutant in all examined doses (P<0.002), proving a key role of anti-proteinase activity for this effect. Therefore, we concluded that PAI-1 apart from the total sprout length affects also sprouts area in the in vitro sprout formation angiogenesis assay. This effect was achieved mainly via PAI-1's anti-proteinase activity.

JAK2 mutation status, hemostatic risk factors and thrombophilic factors in essential thrombocythemia (ET) patients

67%

Sokolowska B., Nowaczynska A., Bykowska K., Chocholska S., Wejksza K., Walter-Croneck A., Gromek T., Kowalska A. M., Kandefer-Szerszen M., Dmoszynska A.

Folia Histochemica et Cytobiologica

2011

tom 49

nr 2

The activity of enzymes of different subcellular localization in the wall of aortic aneurysm and parietal thrombus

67%

Gacko M., Worowska A., Glowinski S.

Bulletin of the Polish Academy of Sciences. Biological Sciences

2000

tom 48

nr 3

Ograniczanie wyników

1 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Folia Histochemica et Cytobiologica

1 Journal of Physiology and Pharmacology. Supplement

1 Bykowska K.

1 Chocholska S.

1 Chorostowska-Wynimko J.

1 Dmoszynska A.

1 Gacko M.

1 Glowinski S.

1 Gromek T.

1 Jankun J.

1 Kandefer-Szerszen M.

1 Kowalska A. M.

1 Nowaczynska A.

1 Skrzypczak-Jankun E.

1 Sokolowska B.

1 Walter-Croneck A.

1 Wejksza K.

1 Worowska A.

1 2011

1 2004

1 2000

Wyniki wyszukiwania

Plasminogen activator inhibitor type-1 controls the process of the in vitro sprout formation

JAK2 mutation status, hemostatic risk factors and thrombophilic factors in essential thrombocythemia (ET) patients

The activity of enzymes of different subcellular localization in the wall of aortic aneurysm and parietal thrombus