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The plasma membrane is a specialised multi-component structure with inter- and intracellular signalling functions. Ca2+ plays a crucial role in cellular physiology, and an ATP-driven plasma membrane calcium pump (PMCA) plays the greatest role in the maintenance of a low free Ca2+ concentration in the cytoplasm. The enzyme is coded by four separate genes (PMCA 1-4), and, due to alternative splicing, more than 20 variants can exist. PMCA 1 and 4 isoforms are present in almost all tissues, whereas PMCA 2 and 3 are found in more specialised cell types. The variants differ primarily in their regulatory regions, thus the modulation of calcium pump activity strongly depends on the isoform and the membrane composition. The unique function of PMCA isoforms was confirmed using the practical experimental models - a rat pheochromocytoma cell line, a human neuroblastoma cell line, or, more recently, knockout mice. In addition, based on the finding that PMCA could interact with several specific signaling proteins, it was concluded that its location in defined sites of the cell membrane could be a prerequisite for efficient intercellular communication.
The substratum photomorphogenesis — the increase of dry weight in light as compared with darkness — takes place only on the nutrient solution with yeast extract. On a synthetic medium with the phosphate level varying from 2.87 to 22.96 mM, the dry weight of mycelia is the same in light and in darkness. In a similar way the phosphorus content in the mycelia and the amount of this element taken up are not related to light conditions. The organism utilizes about 66% of the phosphate present in the nutrient solution.
Biscutella laevigata has recently been recognized as a species able to accumulate large amounts of cadmium. The experiments reported in this paper were conducted on two geographically isolated populations of B. laevigata in Poland. Both populations grow on metalliferous soils: a lead-zinc (calamine) waste heap in Bolesław near Olkusz (189 mg Cd/kg d.m.) and limestone rock in the West Tatra Mts (1.4-6.1 mg Cd/kg d.m.). The two populations were compared after cultivating them in medium containing cadmium salt (2-120 mg/dm3) for 3-30 days. Root-to-shoot transport of cadmium was higher in the waste-heap population than in the mountain population. In the waste-heap population, large amounts of cadmium were transported to the oldest leaves, reaching levels even twice those of the mountain population. This shows that the ability to hyperaccumulate metals may be a property of a population, not an entire species, and that the ability to accumulate cadmium in the oldest (withering) leaves may be a way the plant eliminates the toxic metal. Histochemical detection of cadmium (with dithizone) in tissues showed that it was taken up by the root hairs and then transported through vascular bundles to the leaves. The surface cells of the leaves, the epiderm and hairs accumulated particularly large amounts of cadmium. The leaves of the B. laevigata waste-heap population are much more thickly covered by hairs than those of the mountain population; we suggest that the ability to accumulate cadmium in leaf hairs may be a mechanism of detoxifying and hyperaccumulating cadmium in the shoots of that population.
Maize (Zea mays) growing in soil watered with Sb mine drainage (SMD) could accumulate Sb and transferred Sb from roots to shoots. The photosynthetic processes in the leaves were clearly inhibited by SMD. Chlorophyll synthesis was inhibited. Photosystem II (PSII) was sensitive to SMD. PSII activity (FV/FM and PIABS) was reduced due to SMD. Irrigation with SMD resulted in an increase in the proportion of the closed PSII reaction centers (RCs) and a decrease in the proportion of QB binding centers. The electron transport on both the donor side and the acceptor side was inhibited by SMD. The total electron transport flux in PSII was reduced and the electron transport beyond QA¯ was the primary target site for toxicity of SMD. PSII RCs were transformed into dissipative sinks for excitation energy under stress of SMD. Sb accumulation in crops due to SMD irrigation may not only reduce crop growth and yield, but also pose a threat to food security and human health.
Rats were offered ad libitum zinc in twice the level of the standard diet (23 mg/kg diet), and bentonite (2% additive) for 28 d, together with traces of zinc chloride (labelled with zinc-65) given intragastrically. Results provided evidence that bentonite increased the body retention of zinc in comparison to the diet without this agent. Furthermore, the addition of bentonite did not influence feed intake, organ to body ratios, and haematological values, although a visible decrease in body weight gains following bentonite feeding was noted. These findings may be useful when bentonite fertilised diet is provided to animals pastured in areas with higher zinc levels.
Potato tubers obtained from a field experiment carried out in 2002-2004 were used for our analysis. The soil grain-size structure was that of light loamy sand. Two soil tillage systems were compared: conventional (ploughing + fall ploughing + harrowing + cultivating + harrowing) and simplified (reversing ploughing + cultivating). The second factor involved seven weeding methods such as application of the following herbicides: Plateen 41.5 WG, Plateen 41.5 WG + Fusilade Forte 150 EC, Plateen 41.5 WG + Fusilade Forte 150 EC + Atpolan 80 EC, Barox 460 SL, Barox 460 SL + Fusilade Forte 150 EC, Barox 460 SL + Fusilade Forte 150 EC + Atpolan 80 EC. Iron content and uptake with tuber yield significantly depended on the tillage practices and weeding methods as well as the weather conditions over the growing period. Conventionally tilled potato tubers had more iron, 65.80 g⋅kg-1 on average, than the tubers whose cultivation was based on simplified tillage, 64.82 g⋅kg-1 on average. The herbicides applied significantly increased the iron content and its uptake with tuber yield by an average 4.2 and 13.8%, respectively.
In a two-factor pot experiment, which was conducted 2004-2005, the direct and successive impact was estimated of mixed application of different doses of municipal sewage sludge (0.5, 1.0, 1.5 and 2.0% d.m. of sewage sludge relative to 6 kg d.m. soil in pot) and a constant dose of wheat straw (30 g d.m. per pot), with and without supplemental mineral fertilization with nitrogen and NPK, on the content, uptake and utilization of copper, manganese and zinc by test plants. The soil used in the experiment was brown acid incomplete soil (good rye complex) and the test plant in the first year of research was grass – Festulolium, which was harvested four times, and in the second year – common sunflower and blue phacelia. In mean object samples of Festulolium, common sunflower and phacelia, content of copper, manganese and zinc was marked with the ASA method after mineralization in a mixture of nitric (V) and perchloric acid (VII). Rising doses of municipal sewage sludge with addition of a fixed dose of wheat straw, both in direct and successive effect, increased the content of copper, manganese and zinc in test plants. The increase in the weighted mean (from four swaths) content of copper in Festulolium, in comparison with the control object, varied from 8.04 to 59.8%, manganese from 21.8 to 68.8% and zinc from 19.4 to 59.1%. In the second year, the mean increase in the content of copper in common sunflower from objects fertilized with sewage sludge and straw varied from 8.7 to 30.3% and in phacelia from 6.1 to 12.6%. By analogy, the mean content of manganese rose from 23.3 to 59.5% and from 5.9 to 33.1% and the content of zinc from 33.2 to 50.3% and from 15.9 to 37.9%. Mineral fertilization with N and NPK, in comparison with the object without that fertilization, in both years of the experiment, increased the mean content of all microelements in test plants, with the increase being larger after NPK than N fertilization. The uptake of microelements by plants from sewage sludge and straw, in most cases, was increasing along with the increase of the doses of sewage sludge. In the total uptake of individual microelements, about 2/3 were atken up by Festulolium and the remaining 1/3 by phacelia. Utilization of individual microelements from sewage sludge and straw was considerably diverse. IIn the two years, test plants utilized manganese mostly (on average 58.2%), less zinc (on average 5.54%) and to the smallest degree copper (on average 3.03%).
Field experiment was carried out in the Didactic and Experimental Department in Swadzim near Poznań, in years 2004-2005 (52°26’ N; 16°45’ E). The experiment was established in a „split-plot” design with two factors and four field replications. The primary factor consisted in three nitrogen doses: 0, 60, and 120 kg N·ha⁻¹, while the secondary factor included four doses of kieserite (magnesium sulphate): 0 kg fertilizer·ha⁻¹, 100 kg fertilizer·ha⁻¹ (25 kg MgO·ha⁻¹ + 20 kg S·ha⁻¹), 200 kg fertilizer·ha⁻¹ (50 kg MgO·ha⁻¹ + 40 kg S·ha⁻¹), and 300 kg fertilizer·ha⁻¹ (75 kg MgO·ha⁻¹ + 60 kg S·ha⁻¹). It was found that the fertilization of maize with kieserite in the dose of 300 kg of fertilizer per ha does not generate any disturbances in the correct germination of maize seeds and that such a dose level is not toxic to maize plants. The level of nitrogen fertilization differentiated the dry matter of a single plant and the dry matter yield of plants in the phase of 5-6 leaves (BBCH 15-16), as well as the yield of the dry matter of ears and of the whole plants. The size of kieserite dose differentiated the dry matter yield of stover, ears, and whole plants. Uptake of nitrogen, potassium, and calcium in the phase of 5-6 leaves (BBCH 15-16) increased with the increasing doses of nitrogen. Fertilization of maize with nitrogen in the dose of 120 kg N·ha⁻¹ with kieserite increased the yield of plant dry matter and nitrogen uptake in the above mentioned yield of plants in the phase of 5-6 leaves (BBCH 15-16). The level of nitrogen fertilization differentiated the chlorophyll content expressed in SPAD units in the phase of 5-6 leaves (BBCH 15-16); however, in the ear blooming phase (BBCH 67), the value of this feature was modified by the level of the doses of nitrogen and kieserite.
The paper discusses fluctuations, and their underlying causes, in the magnesium content of meadow sward from a fertilizer experiment set up on a mountain meadow in 1968. The fertilization experiment comprised 8 different treatments, with the following modifications: liming, magnesium and microelement fertilization and a change in the sward use made during the experiment so as to regenerate the sward and to improve the yielding potential. In the initial period, magnesium concentrations in the sward were assessed using the ASS technique after dry mineralization of samples and dissolution of the residue in diluted nitric acid. Later, the ICP technique was applied, including an internal reference sample for the methods. The cultivation measures caused variations in magnesium concentrations in the sward, depending on a number of factors, among which direct fertilization was not the most significant one. For example, the ambient temperature during the plant growing season strongly influenced the magnesium content, irrespectively of a cut. There was no effect of rainfall of the magnesium content, although atmospheric precipitation was able to raise considerably the charge of this element. In the second cut and under neutral soil pH, higher concentrations of magnesium were noted in the sward. This could have resulted from either the lower second swath yielding or the optimum conditions for the uptake of magnesium by the sward that occurred during its growth. The investigation results failed to provide an unambiguous answer to the question whether magnesium fertilization affected the concentrations of this element. Nonetheless, in order to maintain the production of valuable fodder it is essential to return systematically the elements taken up with plant yield to the soil. In intensively managed meadows, amounts of magnesium removed from the soil with the harvested biomass can be as high as several kilograms per hectare.
The study assessed the effect of various forms of sulphur (Na2S2O3, elemental S and Na2SO4) and nitrogen (UAN-30, NH4NO3) on calcium and magnesium content and uptake in spring wheat and cocksfoot. A two-year pot experiment was conducted on soil material of clayey silt granulometric composition. Before the experiment, the soil was characterized by slight acidity and low content of assimilable forms of phosphorus, potassium, magnesium and sulphur. The results show that the experimental factors caused noticeable variation in the content and uptake of calcium and magnesium in spring wheat and cocksfoot. Among the sulphur fertilizers, the greatest increase in calcium and magnesium content and uptake was produced by application of sodium sulphate. The increase in calcium and magnesium uptake was more marked in the case of plants fertilized with nitrogen in the form of ammonium nitrate. The effect of nitrogen fertilization on the analyzed parameters also depended on the species of plant. Wheat grown in the series with ammonium nitrate was characterized by higher Ca and Mg content and uptake than plants fertilized with nitrogen in the form of liquid UAN 30. Calcium uptake by wheat fertilized with NH4NO3 was on average 15% higher for grain and 9% higher for straw compared to wheat fertilized with UAN 30. As for magnesium, the increase was 24.5% and 18%, respectively. Concerning cocksfoot, fertilization with UAN 30 had a greater affect on content and uptake of the analyzed nutrients than ammonium nitrate.
Berkheya coddii Roessler (Asteraceae), an endemic herbaceous and perennial nickel-hyperaccumulating plant growing on Ni-enriched ultramafic soils in South Africa, is perceived as a promising species for phytoremediation and phytomining due to its large biomass production and high Ni content. Total concentrations of a number of elements in mature leaves, soil and related bedrock were obtained. The average Ni concentration in leaves was 18,000 µg · g-1 dry mass, whereas in soil and bedrock the total amount of Ni was 1,300 µg · g-1 and 1,500 µg · g-1, respectively. Exceptionally high average Ni concentrations (55,000 ± 15,000 µg · g-1, n = 6) were found in B. coddii leaves from Songimvelo Game Reserve, including the highest-ever reported concentration of Ni in leaves (76,100 µg · g-1 - maximum value in a single sample). Young plants grown in pots with ultramafic soil accumulated small quantities of Cd, Pb or Zn, but the concentrations of these elements increased after the addition of metal solutions to the soil. Excised shoots immersed in concentrated solutions of Cd, Ni, Pb or Zn accumulated large amounts of these metals in the leaves.
This paper presents the effects of soil supplementation with different forms of nitrogen fertilizers on select fertilization efficiency indices in two types of maize hybrids and on changes in contents of mineral nitrogen in soil after maize harvest. Nitrogen content in grain of maize and its uptake with grain yield and utilization were significantly higher for slow-release fertilizers in comparison to quick-release nitrogen fertilizers. The application of 25 kg MgO·ha-1 significantly increased nitrogen uptake with grain yield and its utilization in comparison to the object with no application of this macronutrient. Better indices of nitrogen use efficiency were recorded for the stay-green hybrid cv. ES Paroli in comparison to the traditional cv. ES Palazzo. Irrespective of the tested experimental factors, the percentage of nitrogen absorbed from the fertilizer in the total amount of nitrogen uptake was less than 25%. The advantage of the stay-green hybrid in comparison to the traditional cultivar in terms of nitrogen content in grain, the uptake of nitrogen with grain yield, and utilization of nitrogen were significantly higher for slow-release nitrogen fertilizers. The combined application of ammonium sulphate in fertilization with 25 kg MgO·ha-1 caused a negative increment in nitrogen uptake and utilization in relation to other nitrogen fertilizers. Significantly less mineral nitrogen (Nmin) in soil after maize harvest was detected in objects on which slow-release fertilizers (e.g. ammonium sulphate and urea) were used in comparison to quick-release fertilizers (e.g. ammonium nitrate). The application of 25 kg MgO·ha-1 in comparison to the object, where this macronutrient was not used, is a cultivation measure causing lower burden of this biogen for the natural environment. The stay-green hybrid ES Paroli in relation to cv. ES Palazzo reduced nitrogen eutrophication of the natural environment with mineral nitrogen. Only nitrate nitrogen was highly correlated with total mineral nitrogen in soil at each depth of soil sample collection. The use of nitrogen fertilizers caused an increase in the contents of nitrate nitrogen in the total amount of mineral nitrogen in the 0- 30 cm and 30-60 cm soil profiles. In turn, the application of magnesium and the stay-green cultivar reduced the amount of N-NO3 in the total amount of Nmin in the period after the maize harvest.
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