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The effect of UV-C light as a means of control of natural mycobiota of grains of maize (Zea mays L.) hybrids “San Juan” and ”H-159” (productive cycle, 2009) was investigated. UV-C lamps of 15 W were used and the exposure times applied were 0, 5, 10, 15, 20, 25 and 30 min. The experiment was established in the randomised complete block design with eight and four replicates. The unit pilot was 50 grains. For the determination of mycobiota the agar plate test was used, after disinfection of the grains with sodium hypochlorite diluted to 3% for 1 min. Differences (P ≤ 0.001, P ≤ 0.05) between treatments were found, the best treatments being those of 30 and 10 min, observing reductions of 42.85 and 52.05% in the number of grains infected with Fusarium spp. with respect to control for “San Juan” and “H-159”, respectively. For Fusarium monoliforme it was found that in 30 min there was a reduction of 53.74% for the hybrid “San Juan”, while for H-159 a reduction of 61.7% in 10 minutes was observed. These results show that UV-C radiation may be useful for application as a germicide in future experiments on a wide variety of grains.
In this study, we sought to find and employ positive effects of UV-A irradiation on cultivation and quality of microgreens. Therefore, the goal of our study was to investigate the influence of 366, 390, and 402 nm UV-A LED wavelengths, supplemental for the basal solid-state lighting system at two UV-A irradiation levels on the growth and phytochemical contents of different microgreen plants. Depending on the species, supplemental UV-A irradiation can improve antioxidant proper­ties of microgreens. In many cases, a significant increase in the investigated phytochemicals was found under 366 and 390 nm UV-A wavelengths at the photon flux density (12.4 μmol m-2 s-1). The most pronounced effect of supplemental UV-A irradiation was detected in pak choi microgreens. Almost all supplemental UV-A irradiation treatments resulted in increased leaf area and fresh weight, in higher 2,2–diphenyl–1–picrylhydrazyl free-radical scavenging activity, total phenols, anthocyanins, ascorbic acid, and α-tocopherol.
The mechanism by which the mitochondrial large rRNA is involved in the restoration of the pole cell-forming ability in Drosophila embryos is still unknown. We identified a 15-ribonucleotide sequence which is conserved from the protobacterium Wolbachia to the higher eukaryotes in domain V of the mitochondrial large rRNA. This short sequence is sufficient to restore pole cell determination in UV-irradiated Drosophila embryos. Here, we provide evidence that the conserved 15-base sequence is sufficient to restore luciferase activity in vitro. Moreover, we show that the internal GAGA sequence is involved in protein binding and that mutations in this tetranucleotide affect the sequence’s ability to restore luciferase activity. The obtained results lead us to propose that mtlrRNA may be involved either in damaged protein reactivation or in protein biosynthesis during pole cell determination.
The combined effects of enhanced UV-B radiation and soil drought on antioxidant enzyme activity were investigated in cucumber leaves. One-month-old cucumber plants (Cucumis sativus cv. Dar) were exposed to UV-B irradiation and water deficit alone or combined. Physiological measurements were made in seedlings kept under stress conditions for nine days and then two more days with stresses withdrawn. Generally a decrease in relative water content and an increase in dry weight content were recorded. The more significant changes were observed under drought than under UV-B radiation and or combined UV-B and drought. Both stresses stimulated antioxidant enzyme activity. Superoxide dismutase activity increased earlier (day 2) than guaiacol peroxidase and glutathione reductase activity (days 5 and 7). Elevation of enzyme activities was higher under drought than under UV-B. Combined UV-B and drought functioned synergistically: one of the stresses reduced the effects caused by simultaneous application of the other.
Disinfection of anaerobically treated municipal wastewater was done using ozone, UV, H2O2, peracetic acid, and advanced oxidation processes (O3/H2O2, O3/UV, H2O2/UV, H2O2/sunlight). Ozone, UV irradiation, and H2O2 eliminated 99% of pathogens (total coliform, fecal coliform, and E. coli) at ozonation time of 20 min at the rate of 1000 mg/hr; UV irradiation time of 11 min and H2O2 dose of 336 mg/L. The use of combined systems (H2O2/sunlight) is especially promising due to the synergistic effect and cost efffectiveness. Regrowth of pathogens increased with increase in time and temperature (from 15 to 35ºC). The presence of nutrients and fluorescent light enhanced the rate of reactivation of pathogens.
Photopheresis (ECP) is an immunomodulatory therapy that involves extracorporeal exposure of isolated peripheral blood leukocytes to UVA irradiation in the presence of 8-methoxypsoralen (8-MOP) followed by their reinfusion to the patient. However, the underlying mechanism of ECP is not well understood yet. We selected 8-methoxypsoralen (8-MOP), chlorpromazine (CPZ) and 4,6,4'-trimethylangelicine (TMA) because of differences in their ability to induce immune suppression in rats in vivo. In this study, we investigated the role of UVA irradiation of lymphocytes in the presence of TMA, CPZ or 8-MOP on cell apoptosis, and their impact on adhesion of lymphocytes to monocytes in vitro. Apoptosis of lymphocytes and their sub-populations (lymphocytes T and B, NK cells) were determined by a flow cytometry, using AnnexinV-FITC, TUNEL assay and DNA content analysis and antibodies CD3, CD56, CD19. Mitochondrial potential was measured using CMXRos staining and the interaction of monocytes with lymphocytes was monitored by PKH26 Red Sigma staining of lymphocytes and subsequent use of flow cytometry. Our results show a significant increase of apoptosis of the photochemically treated lymphocytes and a decrease of their mitochondrial potential that depended on the dose and time after the treatment. Our data also reveal an increased recognition of apoptotic lymphocytes by freshly isolated monocytes.
The resistance of edible oils to oxidative degradation, leading to rancidification, is an important parameter for assessing the quality of oil. This paper presents a new promising method to diagnose the volatile compounds which are products of oil oxidation reactions. Our experiment was carried out using a combined method of UV irradiation as an oxidation acceleration technique, headspace solid-phase microextraction (HS-SPME) as an extraction technique and capillary gas chromatography (GC) as an assay technique. Method precision and sensitivity expressed as RSD (<19%) and LOD (between 18 and173 ng/mL) are satisfactory. The induction period set on hexanal / t-2-nonenal ratio (IP=6.67 h) is comparable with those obtained with the Rancimat method (4.64–6.73 h).
Irradiation of organisms with UV light produces genotoxic and mutagenic lesions in DNA. Replication through these lesions (translesion DNA synthesis, TSL) in Escherichia coli requires polymerase V (Pol V) and polymerase III (Pol III) holoenzyme. However, some evidence indicates that in the absence of Pol V, and with Pol III inactivated in its proofreading activity by the mutD5 mutation, efficient TSL takes place. The aim of this work was to estimate the involvement of SOS-inducible DNA polymerases, Pol II, Pol IV and Pol V, in UV mutagenesis and in mutation frequency decline (MFD), a mechanism of repair of UV-induced damage to DNA under conditions of arrested protein synthesis. Using the argE3→Arg+ reversion to prototrophy system in E. coli AB1157, we found that the umuDC-encoded Pol V is the only SOS-inducible polymerase required for UV mutagenesis, since in its absence the level of Arg+ revertants is extremely low and independent of Pol II and/or Pol IV. The low level of UV-induced Arg+ revertants observed in the AB1157mutD5DumuDC strain indicates that under conditions of disturbed proofreading activity of Pol III and lack of Pol V, UV-induced lesions are bypassed without inducing mutations. The presented results also indicate that Pol V may provide substrates for MFD repair; moreover, we suggest that only those DNA lesions which result from umuDC-directed UV mutagenesis are subject to MFD repair.
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