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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

100%
Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
|
2012
|
tom 58
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nr 4
A concentration of two phenylethanoids in the roots of two species: Rhodiola kirilowii and rosea were compared, aqueous and hydroalcoholic extracts from those plants were also analyzed. In order to determine the content of p-tyrosol and salidroside, the ultra performance liquid chromatography connected with a tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The obtained results shown that content of measured phenylethanoids depends on Rhodiola species. Roots of R. kirilowii contain more p-tyrosol, while R. rosea roots are reacher in salidroside. Our results indicate that the application of UPLC MS/MS method allows to determine the phenylethanoids content in tested samples with satisfactory precision.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

72%
Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.
Herba Polonica
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2012
|
tom 58
|
nr 3
The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method

72%
Gryszczynska A., Krajewska-Patan A., Dreger M., Buchwald W., Pietrosiuk A., Zych M., Karasiewicz M., Bogacz A., Kujawski R., Furmanowa M., Czerny B., Mielcarek S., Gazda P., Mrozikiewicz P. M.
Herba Polonica
|
2012
|
tom 58
|
nr 4
Several species of Rhodiola genus (Crassulaceae family) like Rhodiola kirilowii and Rhodiola rosea are used in official or traditional medicine. The aim of this study was to determine qualitative and quantitative content of proanthocyanidins using ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) in the callus tissues and in the transformed roots (infected by Agrobacterium rhizogenes LBA 9402 strain) of R. kirilowii and R. rosea. This validated assay allows to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG). Our results concerning the material from in vitro cultivation of R. kirilowii and R. rosea indicate that R. rosea callus can be a better source of catechin when compared with other tested materials, especially when the content of (-)-gallate epigallocatechin is taken under consideration (3.429 mg/100 g of dry powdered material). The application of UPLC MS/MS method allowed to determine the content of proanthocyanidins in tested samples with satisfactory precision and can be used in the phytochemical investigations of Rhodiola sp. in vitro cultivated tissues.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Determination of chlorogenic and gallic acids by UPLC-MS/MS

72%
Gryszczynska A., Opala B., Lowicki Z., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Boron D., Bogacz A., Mrozikiewicz P. M.
Herba Polonica
|
2013
|
tom 59
|
nr 1
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Novel method of detection of phenylpropanoids of Rhodiola roots species

72%
Gryszczynska A., Opala B., Krajewska-Patan A., Lowicki Z., Buchwald W., Mielcarek S., Bogacz A., Karasiewicz M., Boron D., Czerny B., Mrozikiewicz P. M.
Herba Polonica
|
2013
|
tom 59
|
nr 1
The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.
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