From the surveyed several Brassica oleracea varieties, two viruses have been isolated and identified, namely cauliflower mosaic caulimovirus (CaMV) and tumip mosaic potyvirus (TuMV). Diagnosis was based on the host range, including test plants, on virus properties in sap and in purified virus suspension, on serological tests and on electron microscopic examinations. The third virus, broccoli necrotic yellows cytorhabdo-like virus (BNYV), was detected sporadically, only by electron microscopic observations. It occurred always together with TuMV and/or CaMV. Its isolation was not successful.
Most of the plantations of different Brassica oleracea varieties surveyed in the central and west-north region of Poland, appeared to be virus infected. All but two, from among 9 varieties observed, were more or less virus infected. Mainly two viruses have been found in observed fields: TuMV and CaMV. In general, the former one was more prevalent than the latter one. CaMV more often was detected in cauliflower and broccoli fields, whereas TuMV - in fields of Brussels sprouts and red, white and Savoy cabbages. Occasionally BNY-like virus was detected always together with CaMV or/and TuMV.
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