Wyniki wyszukiwania

1

Effects of acaroid mites infesting tulip bulbs during storage

100%

Czajkowska B.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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2003

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tom 51

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nr 1

2

Health status of two plantations of tulip near Pulawy and fungi isolated from foliar parts and bulbs

80%

Piwoni A.

Electronic Journal of Polish Agricultural Universities. Series: Horticulture

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2007

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tom 10

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nr 4

3

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Effects thidiazuron and paclobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication

80%

Podwyszynska M., Marasek A.

Acta Societatis Botanicorum Poloniae

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2003

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tom 72

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nr 3

The effects of TDZ and paclobutrazol on the primary regeneration on tulip flower stalk explants of six cultivars and subsequent shoot multiplication were examined. Explants, flower stalk slices, were excised from cooled and subsequently forced bulbs. The explants were incubated for two months in darkness on medium containing NAA and cytokinins, 2iP and BAP, as control, or TDZ (0.5-4 mg l-1) and paclobutrazol (0.05-0.4 mg l-1). Then, the regenerating explants were subcultured on medium with TDZ and NAA applied at low concentrations. Different regeneration capabilities were found depending on cultivar and growth regulators. The percentage of explants forming leaf-like structures ranged, on the control medium, from 80% in 'Blue Parrot' and 'Prominence' to below 30% in 'Apeldoorn' and 'Mirjoran'. TDZ, applied at optimum for each cultivar concentration, greatly increased the regeneration potential up to 70-100%. Paclobutrazol, added to the TDZ-containing medium, significantly enhanced the response of explants, resulting in high numbers of leaf-like structures formed per explant (13.7-22.8). The structures developed gradually into characteristic forms: the growing up cotyledonary leaf, the probable root primordium formed at its base, the growing downwards stolon and the shoot meristem developed finely on its tip. It is suggested that such primary regeneration may have a nature of somatic embryogenesis. Then, the adventitious shoots developed and formed clusters, which were divided into 2-3 smaller ones every two months. The growth regulators, used at initial stage, markedly influenced subsequent shoot multiplication. Thus, the most intensive shoot formation was noted with TDZ at concentrations of 0.5-2 mg l-1 and paclobutrazol of 0.05-0.1 mg l-1.

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Electronic Journal of Polish Agricultural Universities. Series: Horticulture

1 Czajkowska B.

1 Marasek A.

1 Piwoni A.

1 Podwyszynska M.

1 2007

2 2003

Effects of acaroid mites infesting tulip bulbs during storage

Health status of two plantations of tulip near Pulawy and fungi isolated from foliar parts and bulbs

Effects thidiazuron and paclobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication