Wyniki wyszukiwania

1

Transforming growth factors (TGFs) produced by R-XC cells

100%

Klein A., Branny J.

Folia Histochemica et Cytobiologica

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1985

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tom 23

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nr 4

2

Some remarks on factors influencing on the effectiveness and specificity of the unlabeled peroxidase-antiperoxidase method in immunohistochemistry

100%

Kaluza J., Stachura J.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

3

Matrix degrading proteinases from human granulocytes: type I, II, III collagenase, gelatinase and type IV, V-collagenase. A survery of recent findings and inhibition by gamma-anticollagenase

86%

Tschesche H., Fedrowitz J., Kohnert U., Michaelis J., Macartney H. W.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

4

Explant-like passaging of cells growing on portable substrata permits the avoidance of enzyme application and facilitates the passage procedure

86%

Ryszawy D., Podulka J., Tworzydlo A., Tomassy J., Jarmulski M., Madeja Z., Korohoda W.

Folia Biologica

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2019

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tom 67

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nr 4

5

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Bio-inspired trypsin-chitosan cross-linked enzyme aggregates: a versatile approach for stabilization through carrier-free immobilization

86%

Mageed H. A., Ezz N. A., Radwan R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

6

Studies on biochemical indicators of virulence in the Amoebae of the Acanthamoeba sp.

86%

Hadas E., Mazur T.

Acta Protozoologica

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1991

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tom 30

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nr 3-4

7

Immunoreactive properties of pea protein extract and its trypsin hydrolysates

86%

Fraczek R. J., Kostyra E., Kostyra H., Krawczuk S.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 3

472-484

8

The ghrelin pentapeptide inhibits the secretion of pancreatic juice in rats

86%

Kapica M., Laubitz D., Puzio I., Jankowska A., Zabielski R.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 4

Ghrelin, a 28 amino acids polypeptide was recognized as an endogenous ligand for the growth hormone secretagogue receptor. It turned out that the entire sequence of ghrelin is not necessary for performing the above-mentioned functions. It was suggested that 5 residues (Gly-Ser-Ser(n-octanoyl)-Phe, pentaghrelin) constituted functionally active part of the full-length polypeptide. Ghrelin-28 was found to inhibit pancreatic enzyme output in rats, though the effect of pentaghrelin was not studied so far. The study aimed to determine the involvement of pentaghrelin in pancreatic juice secretion in anaesthetized rats. Male Wistar rats (220 ± 20 g body weight, b. wt.) were anesthetized, the external jugular vein and common biliary-pancreatic duct were cannulated. Pentaghrelin boluses (iv, 1.2, 12, and 50 nmol kg-1 b. wt.) were injected every 30 min with or without CCK-8 infusion, duodenal mucosal CCK1 receptor blockade with tarazepide, vagotomy and capsaicin pretreatment. Pentaghrelin boluses reduced the volume of pancreatic-biliary juice, protein and trypsin outputs both under basal and CCK-8-stimulated conditions in a dose-dependent manner. However, exogenous pentaghrelin failed to affect the pancreatic secretion in rats subjected to vagotomy, capsaicin deactivation of afferents or pretreatment with Tarazepide. In conclusion, pentaghrelin may control exocrine pancreas secretion by affecting duodenal neurohormonal mechanism(s) involving CCK and vagal nerves in rats.

9

Alterations in the susceptibility to trypsinolysis of proteins chlorinated and- or exposed to SIN-1

86%

Sieradzka-Fleituch M., Pajdak W.

Current Topics in Biophysics

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1994

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tom 18

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nr 2

10

Effect of fundectomy, antrectomy and gastrectomy on pancreatic and brush border enzyme activity in rats

72%

Kapica M., Puzio I.

Medycyna Weterynaryjna

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2018

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tom 74

|

nr 10

The aim of our study was to investigate the possible effects of the removal of different parts of the stomach (fundectomy, antrectomy, gastrectomy) on the total protein content and enzyme activity in the pancreas and the brush border of the intestinal mucosa. Twenty-four 2.5-month-old male Wistar rats were divided into four groups: sham-operated animals (SHO) and those subjected to gastrectomy (Gx), fundectomy (Fx), and antrectomy (ANT). After a six-week experiment, the rats were sacrificed, and blood was collected for further gastrin analysis in serum. Samples of the pancreas, duodenum, and jejunum (proximal part in 25% of length, middle part in 50% of length, and distal part in 75% of length) were collected to determine the total protein content and enzyme activity. The rats subjected to fundectomy, antrectomy and gastrectomy showed an increased total protein content and enzyme activity (amylase, trypsin) in pancreatic tissue. They exhibited an increase in the total protein content in the homogenates of the mucosa of the proximal, middle and distal jejunum, compared to the control, and a statistical increase in maltase activity. Compared with the control group, the rats subjected to Fx and ANT showed a decreased sucrase activity in the homogenates of the mucosa of the duodenum and of the proximal, middle and distal jejunum. In the gastrectomized rats, there was a statistically significant increase in the total protein content in the homogenates of the mucosa of the jejunum, compared to the control, while the activities of lactase and sucrase were decreased. There was a statistically significant increase in the gastrin level in all experimental groups (Fx, ANT, Gx). We suggest that surgical removal of a part of the stomach radically changes the level of hormones that determine many functions of the organism. Hormonal changes may have an impact on the pancreas and the activity of brush border enzymes.

11

Effects of specific inhibitors on the gene expression of a digestive trypsin in Pieris brassicae L. (Lepidoptera: Pieridae)

72%

Sharifloo A., Zibaee A., Sendi J. J., Jahromi K. T.

Journal of Plant Protection Research

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2018

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tom 58

|

nr 1

Protein digestion in insects relies on several groups of proteases, among which trypsin plays a prominent role. In the current study, larvae of Pieris brassicae L. were fed radish leaves treated with 1 mM concentrations of three specific inhibitors of trypsin: AEBSF.HCl [4-(2- -aminoethyl)-benzenesulfonyl fluoride, monohydrochloride], TLCK (N-a-tosyl-l-lysine chloromethyl ketone) and SBTI (Soybean Trypsin Inhibitor) to find their potential effects on gene expression of trypsin. Initially, RT-PCR analysis revealed a gene of 748 bp responsible for synthesizing the digestive trypsin in P. brassicae larvae. Also, qRT-PCR data indicated a statistically greater expression of trypsin gene in the larvae fed 1 mM concentrations of AEBSF.HCl, TLCK and SBTI than the control. Results of the current study indicated that synthetic inhibitors can not only negatively affect the gene expression of P. brassicae trypsin, but also the insect can activate a compensatory mechanism against interruption of protein digestion by inducing more expression of the gene and producing more trypsin into the midgut lumen.

12

Alkaline trypsin from the viscera and heads of Engraulis anchoita: partial purification and characterization

72%

Lamas D. L., Yeannes M. I., Massa A. E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

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tom 98

|

nr 2

13

Was the serine protease cathepsin G discovered by S. G. Hedin in 1903 in bovine spleen?

72%

Palesch D., Sienczyk M., Oleksyszyn J., Reich M., Wieczerzak E., Boehm B., Burster T.

Acta Biochimica Polonica

|

2011

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tom 58

|

nr 1

In the beginning of the 20th century, enzymes with proteolytic activity were classified as peptidases, Erepsin, and proteases. Among these, pepsin, trypsin, and autolytic enzymes were of the protease class. Spleen-derived proteases were poorly characterized until Sven Gustaf Hedin performed several digestion experiments with bovine spleen. He incubated minced bovine spleen under acidic or neutral conditions and characterized two active proteases; the results were published in 1903. The first protease was named α-protease and was active under neutral conditions. The second was named β-protease and was active under acidic conditions. We replicated Hedin's experiments according to his methods and found, by using activity-based probes to visualize proteases, that the historical α-protease is the present-day serine protease cathepsin G (CatG), which is known to be important in several immune processes, including antigen processing, chemotaxis, and activation of surface receptors. The β-protease, however, comprised different proteases including CatX, B, S, and D. We suggest that Hedin described CatG activity in bovine spleen over 100 years ago.

14

Modification of collagen film by certain chemical agents

72%

Lesiak-Cyganowska E., Sladowski D., Komender J.

Archivum Immunologiae et Therapiae Experimentalis

|

2001

|

tom 49

|

nr 3

247-251

15

Proteinases of Streptomyces fradiae II. Specificity

72%

Galas E., Kaluzewska M.

Acta Microbiologica Polonica

|

1992

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tom 41

|

nr 3-4

16

Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions

72%

Dziekonska-Rynko J., Rokicki J., Jablonowski Z.

Wiadomości Parazytologiczne

|

2002

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tom 48

|

nr 2

The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.

17

Adherence of Pseudomonas aeruginosa to human buccal epithelial cells

72%

Wolska K., Bednarz B., Jakubczak A.

Acta Microbiologica Polonica

|

2003

|

tom 52

|

nr 4

18

The influence of Al3plus ions on pepsin and trypsin activity in vitro

72%

Krejpcio Z., Wojciak R. W.

Polish Journal of Environmental Studies

|

2002

|

tom 11

|

nr 3

The objective of this study was to investigate the influence of the concentration of Al3+ ions and the substrate/enzyme ratio on pepsin and trypsin activity in vitro. The experimental design was a combination of three Al3+ ion concentrations (0.25; 2.5 and 25.0 μg Al3+/ml of reaction solution) and two substrate/enzyme ratios (S/E = 10 and 100 for pepsin, and 100 and 1000 for trypsin). Enzymatic activity was determined by the Folin method based on the reaction of tyrosine with the Folin reagent. It was found that the concentration of Al3+ ions influenced activity of pepsin which increased with the increasing metal ion concentration in the reaction milieu. Al3+ ions did not affect activity of trypsin. Proteolitic activity of pepsin and trypsin depended on substrate/enzyme ratio. Higher concentration of substrate decreased efficacy of enzymatic protein breakdown in vitro.

19

Novel saccharide ligands for serum amyloid P component

72%

O'Sullivan G. M., Raynes J. G.

Folia Histochemica et Cytobiologica

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1992

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tom 30

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nr 4

20

Effect of different levels of cellulose in the diet on the proteolytic activity of the pancreas in broiler chickens

72%

Boguslawska-Tryk M.

Folia Biologica

|

2005

|

tom 53

|

nr Suppl.

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