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The study uses ozone concentrations from stations in Warsaw Agglomeration and its vicinity. Diversity of localizations of considered station, in terms of type of emissivity, allows on comparison of air pollution conditions by ozone in Warsaw area. Concentration of ozone in summer and spring were above twice greater than the concentration in autumn and winter. The greatest differences between weekend days concentration and work days concentration occur during autumn and winter, but in the same time the differences during the day are the least, especially in urban site stations. Statistics analysis shows strong relationship between ozone concentration and nitrogen dioxide concentration and meteorological elements especially for days with high level of ozone concentration. For these days regression equations were statistically signifi cant (α = 1%) and correlation coeffi cients were greater than 0.81. Polynomial of IV power is the best fitted function of one-hourly values of ozone concentration course in particular seasons.
The present study investigated the potential role of selected antioxidant enzymes: superoxide dismutase (SOD) and peroxidase (POX) as biochemical markers of the impact of tropospheric ozone. Experiments were carried out in ambient air conditions with two tobacco cultivars: Bel W3 and Bel B, which are sensitive and resistant to ozone, respectively. In this study, the degree of leaf injury of the sensitive cultivar was used as an indicator of the ozone level in correlation to the enzyme activity of both tobacco cultivars. In spite of low levels of tropospheric ozone during experimental season, the increase of antioxidant enzyme (SOD and POX) activity concomitant with the increase of ozone concentration was noticed in the sensitive cultivar as well as in the resistant one. This observation is especially important for the resistant tobacco, which does not exhibit any visual effects of ozone influence. Our results could be extrapolated to other plant species (i.e. Poaceae, Fabaceae, Solanaceae, Betulaceae, Salicaceae, Pinaceae), which do not reveal visible lesions in response to ozone stress.
Ozone-sensitive and -resistant tobacco plants and an ozone-sensitive bean were employed in this experiment. Plants were exposed at two different sites varying in ozone level, within Poznań city and in a remote forestry area. Net photosynthetic rate (PN), stomatal conductance (gs ) and intercellular CO₂ concentration (Ci ) were measured every 7th day until the 28th day of the experiment. The ozone concentrations were higher at the forest site (called here the high ozone site) than in the city (low ozone site). Mean hour ozone concentrations at forest site varied between 34.2–45.5 ppb and 30.2–39.2 ppb, while cumulative ozone concentrations (AOT 40) were 2032 ppb h⁻¹ and 611, respectively. The aim of the study was to compare the results of exposed ozone-sensitive and resistant test plants according to the potential effect of tropospheric ozone on natural vegetation. The experiment revealed the variability between exposure sites and plant species, as well as changes of photosynthetic parameters during the whole experimental season. Common bean assimilation parameters revealed the best correlation with the tropospheric ozone level. Tobacco test plants – ozone-sensitive and -resistant – varied in response to stress factors. There were no statistical differences between exposure sites and plant response. However, both ozone-sensitive and -resistant tobacco plants revealed lower levels of PN at the low ozone site, while ozone-sensitive bean revealed the opposite relation. Moreover, plants revealed gas exchange relations which were not previously observed in fumigation experiments (such as small differences in Ci levels of tobacco plant in the first three weeks). This might be a result of relatively low levels of tropospheric ozone during the experimental period. Hence, it is suggested to continue ambient air investigations to confirm or reject the results of this experiment.
Eight-week-old tobacco (Nicotiana tabacum L.) Bel W3 (ozone sensitive) and Bel B (ozone resistant) cultivars were exposed to ozone for two weeks at two sites with differing tropospheric ozone levels in five independent series from May 27 to July 25, 2004. After each exposition, the degree of ozone-caused visible leaf damage and the activity of APX, GuPX, and SOD were examined. Visible leaf damage was observed only in the sensitive cultivar; the resistant one did not exhibit any external symptoms. Three-way ANOVA revealed that the activity of all enzymes varied by exposure site, series and cultivar effects. Significant correlations between GuPX activity in the two cultivars and with the degree of leaf damage to the sensitive cultivar were found. This indicates that GuPX activity in the sensitive as well as in the resistant cultivars track changes in tropospheric ozone levels. The positive correlation between ozone level and APX activity in the resistant cv. Bel B, which did not reveal visible symptoms, indicates that this enzyme may contribute to detoxication of H2O2 and alleviation of oxidative damage caused by O3
Podstawę opracowania stanowiły wyniki automatycznych, cogodzinnych pomiarów stężeń ozonu niskotroposferycznego pochodzące ze stacji imisyjnej WIOŚ, zlokalizowanej w Szczecinie przy ul. Andrzejewskiego. Stwierdzono, że w okresie badań obejmującym lata 2005–2007 największymi przeciętnymi stężeniami ozonu charakteryzowała się kalendarzowa wiosna, a najmniejszymi kalendarzowa jesień i zima. W ujęciu tygodniowym wykazano przewagę poziomu imisji weekendowej nad pozostałymi dniami tygodnia najsilniej zaznaczającą się w porze wiosennej. W przebiegu dobowym ozonu, zwłaszcza podczas wiosny i lata, występował wyraźny wzrost stężeń rozpoczynający się w godzinach rannych, z maksimum przypadającym na godziny od 15.00 do 18.00 (wg czasu UTC).
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