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We hypothesise that inhalant exposure to mycotoxins causes developmental outcomes and certain hormone-related cancers that are associated with grain farming in an epidemiological study. The aim of the present study was to identify and validate determinants of measured trichothecene mycotoxins in grain dust as work environmental trichothecene exposure indicators. Settled grain dust was collected in 92 Norwegian farms during seasons of 1999 and 2000. Production characteristics and climatic data were studied as determinants of trichothecenes in settled dust samples obtained during the production of barley (N = 59), oats (N = 32), and spring wheat (N = 13). Median concentrations of trichothecenes in grain dust were <20, 54, and <50 mg/kg (ranges <20-340, <30-2400, and <50-1200) for deoxynivalenol (DON), HT-2 toxin (HT-2) and T-2 toxin (T-2) respectively. Late blight potato rot (fungal) forecasts have been broadcast in Norway to help prevent this potato disease. Fungal forecasts representing wet, temperate, and humid meteorological conditions were identified as strong determinants of trichothecene mycotoxins in settled grain dust in this study. Differences in cereal species, production properties and districts contributed less to explain mycotoxin concentrations. Fungal forecasts are validated as indicators of mycotoxin exposure of grain farmers and their use in epidemiological studies may be warranted.
In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.
W pracy zastosowano technikę MS/MS do oznaczania ośmiu mikotoksyn z grupy trichotecenów stosując chromatograf gazowy sprzężony ze spektrometrem mas firmy Finnigan Mat typ GCQ. Opisano optymalne warunki analizy kolizyjnie indukowanej dysocjacji (CID) i wybrano najlepsze pary jonów (jon pierwotny i jon potomny) dla wszystkich analizowanych toksyn.
Ochratoxin A (OA), zearalenone (ZON), moniliformin (MON) as well as trichotecenes and fumonisines (FUM) are naturally occurring contaminants of cereals and animal feed. They pose a health risk not only to humans but also to livestock and, as a consequence, may cause economical losses either due to unfavorable effects on domestic animals themselves or to an increased potential for health effects in human beings consuming mycotoxin-contaminated edible animal products. At present, large-scale studies are carried out in EU countries to determine a safe, admissible concentration of these toxins in cereals and their processed products. The aim of this review is to collect and summarize information concerning the properties, occurrence and toxicity of these mycotoxins.
W pracy przedstawiono wyniki badań nad biosyntezą w warunkach modelowych trichotecenów tworzonych przez szczepy Fusarium sambucinum. Za pomocą chromatografii gazowej i spektrometrii masowej zidentyfikowano cztery trichoteceny: scirpentriol, 4-monoacetoksyscirpenol, 15-monoacetoksyscirpenol i 4,15-diacetoksyscirpenol. W czasie 21 dni hodowli zaobserwowano w końcowym jej okresie spadek zawartości tworzonego diacetoksyscirpenolu na rzecz 15-monoacetoksyscirpenolu i scirpentriolu, co dowodzi możliwości deacetylacji diacetoksyscirpenolu do scirpentriolu za pośrednictwem układu enzymatycznego Fusarium sambucinum.
Mycotoxins are natural contaminants whose presence in food- and feedstuffs cannot be completely avoided. Since several mycotoxins have been associated with and implicated in human and animal diseases there is a need to establish maximum levels, guidelines or action levels for them in some kinds of commodities. International and government authorities in many countries have been investing in mycotoxins research and initiating administrative actions for elaboration of legislation and implementing regulatory measures for the control of mycotoxins. Codex Alimentarius Commission is established international legislation on food and feed. In European Union specific limits and regulations for mycotoxins and other contaminants are constructed under the general Codex standards and based on proposal from European Commission. The legal basis for European Commission became available with the framework Council Regulation (EEC) No 315/93. In this paper, legislation regarding maximum levels for certain mycotoxins in food- and feedstuffs in European Community and other countries were reviewed and discussed.
This paper describes a HPLC method with DAD detection for determining a mixture of four trichotecenes B in wheat protected by fungicides. The recoveries of trichotecenes B from wheat treated at two levels of fortification: 0.2 and 0.6 mg/kg for NIV, DON, 15-AcDON and 3-AcDON were: 101.83; 75.78; 84.58; 75.19 and 71.17; 70.24; 87.83; 74.95, respectively. The results confirmed usefulness of this method as easy for determining trichotecenes B in plant material.
Mycotoxins are the secondary fungal metabolites, which cause toxic effects on human and animal organisms. They are frequent food contaminants. The most common route of exposure to mycotoxins is ingestion, but it may also involve dermal, respiratory and parenteral routes. These metabolites are produced by various fungal and mould strains like Aspergillus spp., Penicillium spp. and Fusarium spp. The most prominent mycotoxins are aflatoxins, trichothecenes, zearalenone, ochratoxins, fumonisins, patulin, citrinin, alternariol and its monomethyl ether, gliotoxin and beauvericin. There are several diseases, which are mycotoxin-related. Mycotoxins cause acute and chronic intoxications (mycotoxicoses), allergies and tumours. They may demonstrate genotoxic, mutagenic, cytotoxic, teratogenic and carcinogenic properties. Some of them cause strong estrogenic effects and infertility. The others are immunosuppressive, nephrotoxic and hepatotoxic. Taking into account wide range of mycotoxins toxic effects on living organisms, it is established that these compounds are the one of main factors influencing human and animal health.
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