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The aim of this study was to investigate the effects of subchronic exposure to atrazine on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) in Danio rerio. Juvenile growth tests were performed on D. rerio according to OECD method No. 215. For 28 days, fish at an initial age of 30 days were exposed to the environmental atrazine concentration commonly detected in Czech rivers (0.3 μg/L) and a range of sublethal concentrations of atrazine (3.0, 30.0 and 90.0 μg/L). The results showed decreasing growth rates and morphological changes in the liver (dystrophic lesions of hepatocytes) at 90.0 μg/L of atrazine. The environmental concentration of atrazine in Czech rivers did not have any effect on fish growth and development of histopathological changes in D. rerio. The value of NOEC was 30.0 μg/L and the value of LOEC was 90.0 μg/L.
Analyses of the ground waters in respect of presence of residues of plant protection products, i.e. active substances as well as environmental metabolites thereof are performed in the Institute of Plant Protection since the end of 80ties of the past Century. Based on the results obtained in 1993–1994 for 40 wells located in administrative territories of former Poznań, Toruń and Bydgoszcz voivodeships, in the vicinity of intensive agricultural production areas (orchards, farms), wells where significant amounts of residues of triazines group and dealkylated metabolites thereof had been found previously were qualified to further studies. There were 6 wells in which triazine residues were determined most often. Additionally, based on hydrogeological maps, directions of underflows in the areas of well’s locations were determined as well. The aim of the above was to find the additional places for sampling waters distant from pollution sources and estimation of the level of residues of target compounds depending on distance from the basic wells. Seven triazine compounds including basic active substances (atrazine, simazine) and their metabolites [desethyl atrazine, desisopropyl atrazine, desethyldesisopropyl atrazine, hydroxyatrazine and hydroxysimazine] were selected for the presented studies. Residues were analyzed using methodologies designed in the Institute, i.e. solid-phase extraction (SPE) followed by determination by chromatographic techniques HPLC-PDA, GC-NPD and GC-MS. Generally, during 11 years of investigations (1993–2003) samplings were performed 52 times and 323 samples of groundwater including that from additional wells were analyzed. Most often residues of atrazine and deethylatrazine in wells located in environs of Poznań were detected.
In 1985-2002 thirteen weeds resistant to atrazine were selected by a repeated application of triazine herbicides on arable land, in orchards, non-agricultural land and at railways in the Czech Republic. Recently Digitaria sanguinalis biotypes resistant to atrazine have been found at three railway junctions. Long-lasting application of the active ingredient imazapyr at railways caused selection of resistant Kochia scoparia biotypes. High resistance to chlorsulfuron has been discovered in five Apera spica-venti biotypes originating in winter cereals fields. The molecular basis of resistance to atrazine has been identified in the following weeds: Kochia scoparia, Solatium nigrum, Senecio vulgaris, Conyza canadensis, Digitaria sanguinalis, Amaranthus retroflexus and Chenopodium album. The resistance was conferred by a glycine for serine substitution at residue 264 of the D1 protein in all of those weeds. The resistance to imazapyr in Czech Kochia scoparia biotypes was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated, that the mutation induced a leucine for tryptophane substitution. There was excellent correspondence between the phenotypic resistance to herbicides of individual plants and the presence of mutations.
W latach 2004-2006 w Zakładzie Doświadczalno-Dydaktycznym w Swadzimiu koło Poznania przeprowadzono doświadczenia polowe dotyczące skuteczności kilku herbicydów: Azoprim 50 WP (atrazyna), Maister 310 WG (formasulfuron + jodosulfuron), Dual 960 EC (metolachlor), Chwastox Turbo 340 SL (MCPA + dicamba), Aminopielik Gold 530 EW (fluroksypr + 2,4 D), Mustang 306 SE (florasulam + 2,4 D), Titus 25 WG (rimsulfuron), Emblem 20 WP (bromoksynil), Cadu Star SC (isoksuflutol + flufenacet), Successor 600 (pethoxamid) oraz Pledge 50 WP (flumioxazin) i ich mieszanek w zwalczaniu chwastów w kukurydzy cukrowej. Na obiektach kontrolnych dominowały następujące gatunki chwastów: fiołek polny (Viola arvensis), komosa biała (Chenopodium album), samosiewy rzepaku (Brassica napus), chwastnica jednostronna (Echinochloa crus-galli), rdest powojowy (Polygonum convolvulus) oraz przytulia czepna (Galium aparine). Stwierdzono różną skuteczność chwastobójczą herbicydów i ich mieszanek. Najskuteczniejszą okazała się mieszanka Maister 310 WG + Aminopielik Gold 530 EW. Herbicydy Pledge 50 WP i Successor 600 skutecznie ograniczały występowanie fiołka polnego, komosy białej i przytulii czepnej. Najmniej skuteczną była mieszanka preparatów Dual 960 EC + Emblem 20 WP. Najwyższy plon kolb stwierdzono po zastosowaniu herbicydów Maister 310 WG + Aminopielik Gold 530 EW.
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