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Since energy storage is a basic metabolic process, the synthesis of neutral lipids oc­curs in all kingdoms of life. The yeast, Saccharomyces cerevisiae, widely accepted as a model eukaryotic cell, contains two classes of neutral lipids, namely steryl esters and triacylglycerols. Triacylglycerols are synthesized through two pathways gov­erned by the acyl-CoA diacylglycerol acyltransferase Dga1p and the phospholipid diacylglycerol acyltransferase Lro1p, respectively. Steryl esters are formed by the two steryl ester synthases Are1p and Are2p, two enzymes with overlapping function which also catalyze triacylglycerol formation, although to a minor extent. Storage of neutral lipids is tightly linked to the biogenesis of so called lipid particles. The role of this compartment in lipid homeostasis and its interplay with other organelles in­volved in neutral lipid dynamics, especially the endoplasmic reticulum and the plasma membrane, are subject of current investigations. In contrast to neutral lipid formation, mobilization of triacylglycerols and steryl esters in yeast are less characterized at the molecular level. Only recently, the triacylglycerol lipase Tgl3p was identified as the first yeast enzyme of this kind by function. Genes and gene products governing steryl ester mobilization still await identification. Besides biochemical properties of enzymes involved in yeast neutral lipid synthesis and degradation, regulatory aspects of these pathways and cell biological consequences of neutral lipid depletion will be discussed in this minireview.
The fraction of free fatty acids (FFA) is present in skeletal muscles. However, there is almost no data regarding regulation in the content of this intramuscular lipid pool. We took advantage of the isolated muscle preparation to examine whether: a) increasing exogenous concentration of FFA (500µM or 700µM, 30min) b) insulin (10.00 I.U./L, 30min), c) adrenalin (4.4 nM, 30min), or d) contractions (200ms, tetani, 1Hz, 30min), affect the FFA content inside myocytes. Incubation of soleus (S) and extensor digitorum longus (EDL) with increasing concentrations of exogenous FFA (from 500µM to 700µM) resulted in an increase in the total FFA fraction in both muscles studied (by 280.2% and 259.1%, respectively). In contracting muscles FFA pool was significantly reduced both in S (by 73.1%) and in EDL (by 31.1%). Neither stimulation by adrenalin nor insulin affected the total content of FFA fraction in the muscles examined. We conclude that a) increased availability of exogenous FFA at the sarcolemma level results in an increase in the size of intramuscular FFA fraction b) the intracellular FFA fraction is utilized by contracting muscles with regard to the fiber composition and to a greater extent in more oxidative muscles, c) FFA fraction remains stable upon stimulation by insulin or adrenalin.
Extensive research have indicated that commercial conjugated linoleic acid (CLA), fed to laboratory animals, showed several health-related properties. The objective of this study was to assess the effect of CLA on serum lipid profile, plasma malondialdehyde and liver fatty acids profile in Wistar rats fed for 23 d. The experimental diets were: I-AIN-93G - control (C), II- AIN-93G diet + 1.0% CLA. The CLA oil contained 600 g CLA/kg, with equal representation of cis-9,trans-11 and trans-10,cis-12 CLA isomers. The experimental treatments had no effect on rats body weight, total cholesterol, HDL, LDL+VLDL and malondialdehyde. The triacylglycerol (TG) was significantly decreased after CLA supplementation. Liver weight, fat and histology were unchanged in CLA group. Liver cholesterol was insignificantly decreased in CLA diet. Dietary treatments had significant effects of on proportions of SFA and MUFA and PUFA in liver. In conclusion, CLA decreases serum TG. Additionally, liver fat composition were changed after CLA supplementation
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Metabolic effects of dietary apple seed oil in rats

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The aim of this study was to determine the fatty acid profile of apple seed oil and its effects on the caecal functions, blood lipids, and markers of antioxidant status and inflammation in rats. A nutritional experiment was performed on Wistar rats allocated to 3 groups of 8 animals each. The animals were fed with a diet containing different sources of fat: pork lard (group LA), rapeseed oil (group RO) and apple seed oil (group AO). Apple seed oil was rich in linoleic acid and oleic acids (57 % and 32.3 % of total fatty acids, respectively). The short chain fatty acid concentration in the caecal digesta was comparable among all groups, whereas the ammonia concentration was lower in groups AO and RO than in group LA (0.32 and 0.3, respectively vs 0.42 mg/g). The plasma alanine (ALT) and aspartate transaminase (AST) activities also decreased in the AO and RO groups (ALT, 19.34 and 19.81, respectively vs 30,7 U/L and AST, 115.1 and 107, respectively vs 138.3 U/L) The plasma triacylglycerols (TG) concentration and the atherogenic index (ATI) of plasma were significantly decreased in the AO group compared to the LA group (TG, 1.79 vs 2.62 mmol/L and ATI, 0.095 vs 0.313). Apple seed oil is a valuable source of unsaturated fatty acids and its dietary addition has slightly better metabolic effects on rat organism than does rapeseed oil.
Krawczuk I. and Górski J.: The significance of plasma triacylglycerols in metabolism of free fatty acids and triacylgrylcerols in rat skeletal muscles. Acta Physiol. Pol., The continuous uptake of plasma triacyl- gylcerols has been shown to be necessary to maintain the stable resting concentration of free fatty acids and triacylglycerols only in a muscle composed of fast - twitch oxidative gylcolytic fibres.
The samples containing rapeseed oil and palm stearin, both initial blends and interesterified products, crystallised in the ß form. The partial substitution of palm stearin for hydrogenated palm stearin caused that besides the ß crystal form also ß crystal form occurred in the reaction products. The amount of the ß form was the greater, the more palm stearin was replaced with partially hydrogenated stearin. The presence of trans isomers, but not the sample content of palmitic acid, was the main factor that delayed the ß to ß transition. In addition, the amount of ß crystal form was found to be greater in products containing partial acylglycerols (mostly diacylglycerols) besides triacylglycerols than in pure respective triacylglycerols.
W pracy badano skład kwasów tłuszczowych w nowo wyhodowanych odmianach nasion rzepaku. Do analizy pobierano próbki oleju rzepakowego pochodzące z trzech zakładów tłuszczowych. Statystyczna ocena składu kwasu tłuszczowego w olejach pozwala stwierdzić, że jego zmiany zależą od warunków wegetacji rzepaku na danym obszarze.
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