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1

Activity of trehalase in embrionating eggs of Ascaris suum [Nematoda]

100%
Zoltowska K., Dmitryjuk M., Bialowas K.
Folia Morphologica
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1998
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tom 57
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nr 1
2

Trehalase as a possible marker of intestinal ischemia - reperfusion injury

86%
Toth,Jr. S., Pekarova T., Varga J., Tomeckova V., Toth S., Lakyova L., Vesela J.
Acta Biochimica Polonica
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2013
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tom 60
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nr 3
Background: Different pathological affections of the small intestine cause corresponding morphological and functional changes. The present study was aimed to assess intestinal trehalase activities during ischemia and following reperfusion, correlate them with the pathological changes and determine whether trehalase could be used as a biochemical marker of the intestinal ischemia, ischemia - reperfusion injury. Material and methods: Wistar rats, randomly divided into 5 experimental groups (IR) (each n=15), were subjected to one hour mesenteric ischemia followed by 0, 1, 4, 12 and 24 hours of reperfusion. As a control group sham operated animals were used (n=15). The activity of trehalase was determined using an adapted Dahlqwist method. The range of intestinal injury was determined using histological (histopathological injury index and goblet cell quantification) and immunohistochemical (Ki67, InSitu TUNEL) methods. Results: The highest activities of trehalase were recorded in the control group (C=4.42±0.373 μmol/mg/h). The most altered intestinal histology detected in group IR1 was accompanied by the lowest trehalase activity (IR1=0.97±0.209 μmol/mg/h; p < 0.001 C vs. IR1). Improved histological structure in the remaining reperfusion periods correlated with increase in trehalase activity. Almost normal mucosal histological architecture and 72% of the enzymatic activity were restored after 24 hours of reperfusion (IR24=3.20±0.266 μmol/mg/h; p < 0.01 IR1 vs. IR24). Conclusion: The correlation between intestinal histology and trehalase activities during intestinal injury has been shown. Trehalase activity is closely associated with the status of the histological architecture of the small intestine.
3

Sugar content, trehalase activity and trehalose level on drone prepupae of Apis mellifera carnica parasitized with Varroa

72%
Zoltowska K., Lipinski Z., Dmitryjuk M., Myszka A.
Journal of Apicultural Science
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2005
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tom 49
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nr 1
4
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Properties of trehalase from muscles of Ascaris suum [Nematoda]

72%
Dmitryjuk M., Zoltowska K.
Wiadomości Parazytologiczne
|
1998
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tom 44
|
nr 3
5
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Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae

58%
Zoltowska K., Lopienska-Biernat E.
Wiadomości Parazytologiczne
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2006
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tom 52
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nr 2
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
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