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The aim of the study was to identify the species of microorganisms isolated from the uterus of healthy cows (control group) and cows affected with puerperal metritis (PM) before and after an experimental therapy with an immunomodulator and antibiotics versus commonly applied methods (antibiotic + ß-blocker or antibiotic + PGF2a). Examinations were carried out on 110 cows with PM in three farms with similar system of rearing and nutrition. The control group consisted of 21 cows without postpartum disturbances. Smears from the uterus were taken before treatment and then at 21st day of observation. Escherichia coli and other species of Enterobacteriaceae family were isolated from 48.2%of PM cows and 47.6% of healthy cows. The degree of Arcanobacterium pyogenes infection was statistically lower in healthy than in sick cows (9.5% versus 30.0%). Streptococcus sp. was isolated from 13.6% of PM cows and from 16% of control ones. Staphylococci were isolated from 10% of PM and from 30% of control cows. Other bacteria species were isolated from about 10% of the examined cows. The best elimination of infections (66%) was noted in cows treated with the intrauterine antibiotic in combination with PGF2a i.m. injection. Examination showed that species of bacteria in the postparturient uterus were similar in healthy and sick cows. However, Arcanobacterium pyogenes was isolated 3 times more often from the sick animals. This pathogen was identified in 11 cows out of 19 (57.9%) culled subsequently because of infertility after the metritis puerperalis was clinically cured.
The paper aimed at defining the specific biochemical parameters, as well as the use of treatment methods, according to types and intensity of the clinical signs of non-complicated hypophosphataemia in cows. The investigations were carried out on 300 HF cows in 15 herds in the Lublin Region. The mean milk yield in the herds was 36.8 ±2.8 dl. The cows were 3-9 years old and had a similar diet ratio. The animals with hypophosphataemia symptoms in the early stadium (2-7 d) were divided into three groups according to the intensity of clinical signs whereas the control group consisted of cows with no clinical signs of the illness. The inorganic phosphorus (Pi), total Ca, total Mg, K, Fe, total protein, Cu, bilirubin, FFA, and creatinine concentrations, as well as AST and AP activity, were determined in serum and plasma. The animals with markedly exhibited signs (recumbency and tremor) showed significantly decreased Pi, Mg, and FFA contents and simultaneously increased activity of AST and AP. In the group exhibiting minimal signs (group I), there were no significant disturbances in homeostasis except the low Pi concentration, so it was possible to use the specialised phosphorus preparations in the treatment. In the other groups (II, III), which exhibited more marked signs of the illness, it was necessary to improve the parenchymal organs functions in addition to phosphorus level normalisation.
A novel modified Fenton process using heterogeneous catalyst (swarf) was developed to catalyze the oxidation reaction of azo dye Acid Red 18. It was found that swarf could be used to replace iron salts as a catalyst for the Fenton reaction. The examined continuous Fenton process proved to be very efficient for decolorization of simulated wastewater containing 100 mg/dm³ Acid Red 18. Performance of the Fenton reactor and regeneration method of the catalyst was discussed. The simplified mechanism of hydroxyl radical production in the presence of swarf as a catalyst also was proposed.
Slurry, due to high microbiological contamination, requires hygienization before spreading. The agricultural usage of treated slurry has to guarantee biosafety. Therefore, constant monitoring of the slurry treatment process should be conducted. The use of Filter-Sandwich carriers seems to be a prospective solution. The aim of the research was to test whether Filter-Sandwich carriers influence the survivability of microorganisms during the slurry hygienization process and hence, whether they are safe for the environment. Raw cattle and swine slurry with different dry matter content was the research material. Salmonella Senftenberg W775 rods were introduced directly into the slurry and into the carriers placed in the liquid excrements stored at 4 and 20 °C, and underwent anaerobic digestion at 35 °C. The number of tested bacteria obtained from the slurry and carriers was determined using the MPN method with proper microbiological media. The values of physicochemical parameters of the raw and treated slurry were determined, both for the carriers and for slurry only. Biosafety control was also conducted for the carriers in slurry containers. The differences in the theoretical survivability between Salmonella Senftenberg W775 re-isolated from the slurry and the carriers, and in the values of the selected physicochemical parameters obtained at the end of the process, were not statistically significant. The re-contamination of the sterile slurry caused by the bacteria in the carrier was not observed after placement of the carrier with inoculated material. The conducted research proves the usefulness of Filter-Sandwich carriers for continuous hygienization monitoring of the slurry treatment process. This refers not only to the semi-technical scale, but also to the full-scale process.
Many people spend about 80% of their free time practicing sports, including skiing, which in many cases can cause changes in the skeletal system, muscular system and other organs. The aim of this article is to discuss the treatment of a 25-year-old man who suffered a compressive fracture of the C6 vertebra with paresis of the ulnar nerve. The treatment used physiotherapy with changing magnetic fields (magnetostimulation, magnetolaserotherapy, kinesitherapy exercises) with good effects. On the basis of the results we discovered that these treatment methods can accelerate the time of treatment and increase quality of life.
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