Wyniki wyszukiwania

1

Studying of 14-3-3 protein function in transgenic potato

100%

Wilczynski G., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

2

The repression of six 14-3-3 isoforms resulting in the activation of NR and SPS and an increase in the antioxidant properties of transgenic potato plants

100%

Zuk M., Szopa J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

3

Analysis of rabbit fed on wild type and genetic modified potato tubers

100%

Zuk M., Kulma A., Wilczynski G., Prescha A., Szopa J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

4

Antifungal bacteria in the rhizosphere and geocaulosphere of transgenic T4-lysozyme-expressing potato plants

84%

Lottmann J., Berg G.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1999

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tom 47

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nr 2-4

5

ARF protein regulates cAMP synthesis in potato

67%

Kulma A., Wilczynski G., Szopa J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

6

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

67%

Markiewicz E., Wilczynski G., Rzepecki R., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 4

The nuclear matrices of plant cell nuclei display an intrinsic nuclease activity which is capable of nicking supercoiled DNA. Recently a cDNA encoding the 14-3-3 protein from Cucurbita pepo has been cloned and sequenced. The evidence that the recombinant 14-3-3 protein associates with DNase I and endogenous plant nuclease is presented. Evidence is also presented that the cloned 14-3-3 protein isoform, unique in its binding to nuclease within the 14-3-3 family, is located in the nuclei and in the nuclear matrix. Transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita was overexpressed. An increase in tuber number and a decrease in tuber size in the transformants was also observed. The adenine nucleotide pool in leaves of transgenic plants was significantly reduced and they contain more chlorophyll and loose it slower when grown in the dark. A decrease in 14-3-3 protein content concomitant with an increase in nuclease activity in senescent plants was detected and this was markedly delayed in transgenic potato plants which overexpressed the 14-3-3 protein. It is proposed that a function of the isolated 14-3-3 isoform is the control of the nuclease activity and hence senescence.

7

Transformation of diploid potato with an Agrobacterium tumefaciens binary vector system: I. Methodological approach

67%

Borkowska M., Kleczkowski K., Klos B., Jakubiec J., Wielgat B.

Acta Physiologiae Plantarum

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1994

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tom 16

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nr 3

8

Modern approaches to the breeding of potato [Solanum tuberosum L.]

67%

Tacke E., Uhrig H., Rohde W., Gebhardt C., Salamini F.

Biotechnologia

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1996

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nr 1

9

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Field performance of transgenic clones obtained from potato cv.Irga

67%

Flis B., Zimnoch-Guzowska E.

Journal of Applied Genetics

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2000

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tom 41

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nr 2

10

Manipulating of 14-3-3 protein expression resultes in the changes of catecholamine content in potato plant

59%

Wilczynski G., Kulma A., Feiga I., Wenczel A., Szopa J.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 1

Recently transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita pepo was overexpressed or potato endogenous 14-3-3 protein repressed. Detailed analysis of those plants suggested that a isolated 14-3-3 isoforms are involved in the control of plant senescence and carbohydrate metabolism. In this study carbohydrate content, adenine nucleotide level and catecholamine content in tubers and leaves of transgenic plants grown in greenhouse and in a field were compared. Overexpressing of 14-3-3 protein gave rise to increase catecholamine contents and soluble sugars in leaves and a reduction in tubers size and starch content. The repression of 14-3-3 synthesis led to opposite effect, a decrease in catecholamine, soluble sugars contents in leaves, and an increase in tubers size and starch content. It is proposed that 14-3-3 protein affects carbohydrate metabolism in potato via regulation of catecholamine synthesis.

Ograniczanie wyników

Studying of 14-3-3 protein function in transgenic potato

The repression of six 14-3-3 isoforms resulting in the activation of NR and SPS and an increase in the antioxidant properties of transgenic potato plants

Analysis of rabbit fed on wild type and genetic modified potato tubers

Antifungal bacteria in the rhizosphere and geocaulosphere of transgenic T4-lysozyme-expressing potato plants

ARF protein regulates cAMP synthesis in potato

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

Transformation of diploid potato with an Agrobacterium tumefaciens binary vector system: I. Methodological approach

Modern approaches to the breeding of potato [Solanum tuberosum L.]

Field performance of transgenic clones obtained from potato cv.Irga

Manipulating of 14-3-3 protein expression resultes in the changes of catecholamine content in potato plant