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1

The expression of Fasciola hepatica antigens in plants

100%
Czaplinska M., Wedrychowicz H., Plucienniczak A., Legocki A. B.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
2

Factors affecting the production of potential transgenic pigs by DNA microinjection; a six-year retrospective study

86%
Jura J., Smarag Z., Slomski R., Lipinski D., Gajda B.
Journal of Animal and Feed Sciences
|
2007
|
tom 16
|
nr 4
636-645
3

Noninvasive fluorescent screening of microinjected bovine embryos to predict transgene integration

86%
Rosochacki S. J., Kozikova L. V., Korwin-Kossakowski M., Matejczyk M., Poloszynowicz J., Duszewska A. M.
Folia Biologica
|
2003
|
tom 51
|
nr 1-2
4

Production of functional transgenic mice by DNA pronuclear microinjection

86%
Auerbach A. B.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 1
Successful experiments involving the production of transgenic mice by pronuclear microinjection are currently limited by low efficiency of random transgene integra­tion into the mouse genome. Furthermore, not all transgenic mice express integrated transgenes, or in other words are effective as functional transgenic mice expressing the desired product of the transgene, thus allowing accomplishment of the ultimate experimental goal - in vivo analysis of the function of the gene or gene network. The purpose of this review is to look at the current state of transgenic technology, utiliz­ing a pronuclear microinjection method as the most accepted way of gene transfer into the mouse genome.
5

Comparison of transfection methods for porcine zygotes

72%
Jura J., Smorag Z., Slomski R., Lipinski D., Jura J., Gajda B.
Annals of Animal Science
|
2009
|
tom 09
|
nr 3
279-285
6
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Transgenic mammalian species, generated by somatic cell cloning, in biomedicine, biopharmaceutical industry and human nutrition/dietetics - recent achievements

72%
Samiec M., Skrzyszowska M.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
Somatic cell cloning technology in mammals promotes the multiplication of productively-valuable genetically engineered individuals, and consequently allows also for standardization of transgenic farm animal-derived products, which, in the context of market requirements, will have growing significance. Gene farming is one of the most promising areas in modern biotechnology. The use of live bioreactors for the expression of human genes in the lactating mammary gland of transgenic animals seems to be the most cost-effective method for the production/processing of valuable recombinant therapeutic proteins. Among the transgenic farm livestock species used so far, cattle, goats, sheep, pigs and rabbits are useful candidates for the expression of tens to hundreds of grams of genetically- engineered proteins or xenogeneic biopreparations in the milk. At the beginning of the new millennium, a revolution in the treatment of disease is taking shape due to the emergence of new therapies based on recombinant human proteins. The ever-growing demand for such pharmaceutical or nutriceutical proteins is an important driving force for the development of safe and large-scale production platforms. The aim of this paper is to present an overall survey of the state of the art in investigations which provide the current knowledge for deciphering the possibilities of practical application of the transgenic mammalian species generated by somatic cell cloning in biomedicine, the biopharmaceutical industry, human nutrition/dietetics and agriculture.
7

O biotechnologii w rolnictwie

72%
Thomas E.
Biuletyn Informacyjny. Instytut Zootechniki
|
2001
|
tom 39
|
nr 2
47-52
8

The effect of mammary gland-specific transgene expression on rabbit reproductive gland structure

72%
Tvrda E., Massanyi P., Lukac N., Danko J., Schlarmannova J., Chrenek P.
Folia Biologica
|
2014
|
tom 62
|
nr 2
9

Expression of microinjected reporter gene lacZ during first cleavages of rabbit embryos

72%
Rosochacki S. J., Strzelecka M., Kozikova L., Matejczyk M., Oblap R., Poloszynowicz J., Zwierzchowski L.
Folia Biologica
|
2002
|
tom 50
|
nr 1-2
10

Expression of GFP in microinjected bovine embryos

72%
Rosochacki S. J., Duszewska A. M., Kozikova L., Olszewski R.
Animal Science Papers and Reports
|
2001
|
tom 19
|
nr 3
11

Retroviral vectors as tools for animal transgenesis

72%
Lisowski P., Kleczkowski M., Jakubczak A.
Advances in Agricultural Sciences
|
2004
|
tom 09
12

Liposomes in gamete and embryo biotechnology

58%
Grad I.
Annals of Animal Science
|
2010
|
tom 10
|
nr 1
3-8
13

Etyczne aspekty biotechnologii zwierzat

58%
Smorag Z.
Biuletyn Informacyjny. Instytut Zootechniki
|
1996
|
tom 34
|
nr 3
5-10
14

Human antibody expression in transgenic mice

58%
Bruggemann M.
Archivum Immunologiae et Therapiae Experimentalis
|
2001
|
tom 49
|
nr 3
203-208
15

Mice and humans: chromosome engineering and its application to functional genomics

58%
Klysik J.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 3
Functional modeling of human genes and diseases requires suitable mammalian model organisms. For its genetic malleability, the mouse is likely to continue to play a major role in defining basic genetic traits and complex pathological disorders. Re­cently, gene targeting techniques have been extended towards developing new engi­neering strategies for generating extensive lesions and rearrangements in mouse chromosomes. While these advances create new opportunities to address similar ab­errations observed in human diseases, they also open new ways of scaling-up mutagensis projects that try to catalogue and annotate cellular functions of mamma­lian genes.
16

Influence of DNA concentration on the efficiency of bovine blastocyst production after WAP-bGH microinjection into germinal vesicle of immature oocytes, zygotes and embryos obtained in vivo or in vitro

58%
Jura J., Smorag Z., Katska L., Skrzyszowska M., Houdebine L. M., Rynska B.
Biotechnologia
|
1997
|
nr 4
17

Use of HIV as a gene transfer vector

44%
Pluta K., Kacprzak M. M.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 4
531-595
 Despite the extensive research efforts over the past 25 years that have focused on HIV, there is still no cure for AIDS. However, tremendous progress in the understanding of the structure and biology of the HIV virus led to the development of safe and potent HIV-based transgene delivery vectors. These genetic vehicles are referred to as lentiviral vectors. They appear to be better suited for particular applications, such as transgene delivery into stem cells, compared to other viral- and non-viral vectors. This is because Lentivirus-based vectors can efficiently infect nondividing and slowly dividing cells. In the present review article, the current state of understanding of HIV-1 is discussed and the main characteristics that had an impact on vector design are outlined. A historical view on the vector concept is presented to facilitate discussion of recent results in vector engineering in a broader context. Subsequently, a state of the art overview concerning vector construction and vector production is given. This review also touches upon the subject of lentiviral vector safety and related topics that can be helpful in addressing this issue are discussed. Finally, examples of Lentivirus-based gene delivery systems and their applications are presented, with emphasis on animal transgenesis and human gene therapy.
18

Biotechnologia w produkcji miesa

44%
Shwagele F. C.
Biuletyn Informacyjny. Instytut Zootechniki
|
2002
|
tom 40
|
nr 1
11-23
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