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1

Genotyping of recombinant Pichia pastoris strains

100%
Viader-Salvado J. M., Cab-Barrera E. L., Galan-Wong L. J., Guerrero-Olazaran M.
Cellular and Molecular Biology Letters
|
2006
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tom 11
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nr 3
A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.
2

Fasciola hepatica miracidia: Lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation

72%
Georgieva K., Georgieva S., Mizinska Y., Stoitsova S. R.
Acta Parasitologica
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2012
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tom 57
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nr 1
The lectin binding properties of Fasciola hepatica miracidia were studied by a panel of fluorescein- and gold-conjugated lectins (ConA, LCA, WGA, LEA, SBA, HPA and UEA-I). The presence of mannose and/or glucose residues was demonstrated with ConA and LCA as weak diffuse fluorescence of the miracidial surface, which was more intense at the anterior part of the larva. The N-acetylglucosamine-binding lectins WGA and LEA reacted intensely with the whole miracidial surface. No labelling with N-acetylgalactosamine and/or galactose-specific (SBA and HPA) and fucose-specific UEA-I lectins was observed. The possibility that the specific recognition of the miracidial surface carbohydrates by lectins may initiate the process of transformation of the miracidia into sporocysts was examined in vitro in physiological saline for Galba truncatula. Incubation in the presence of ConA and WGA resulted in facilitation of the transformation process. Facilitation was absent in the presence of inhibitor sugars. Incubation in the presence of SBA or UEA-I had no effect. The results suggested a possible impact of carbohydrate-lectin interactions in transformation of miracidia of F. hepatica to sporocysts in vivo.
3

Untypical forms of non-agricultural entrepreneurship in rural areas during economic transition [based on a survey carried out in rural communes in Mazovian voivodship]

72%
Zajac J.
Electronic Journal of Polish Agricultural Universities. Series Economics
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2006
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tom 09
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nr 1
The key role of non-agricultural economic activity in the transformation process as well as in giving up monofunctionality of Polish villages gave rise to the necessity to precisely analyse its character and to define its types. The presented analysis of non-agricultural economic activity was based on interviews with local authorities and on primary data for 1996-2003, gathered from registers of economic activity in offices of thirty-six communes in Mazovian Voivodship (NUTS II). The results of studies showed, however, that complication of social, economic, legal and cultural conditions resulted in quite new types of entrepreneurship run in surveyed communes during transition. The further stage of analysis enabled us to define eight new, untypical (when compared to classical definition) forms of entrepreneurship, which differ considerably from entrepreneurship types described in literature. These are: fictious, constrained and sub-contractual economic activities, economic activity registered alternatively among family members, as well as seasonal, cyclic, occasional and ‘sleeping’ economic activities.
4
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The identification and estimation of local conditions of agrotourism development in rural areas

58%
Palka E.
Infrastruktura i Ekologia Terenów Wiejskich
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2009
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nr 07
An analysis, identification and assessment of the local conditions of agrotourism development in rural areas is performed in the paper. The important role of tourism both in the state policy and regional policy have been shown in the state and regional documents. In most of European countries off-farming activities become more and more important as a source of income for farmers' families. In some regions agrotourism can be regarded as a significant non-agricultural activity. In Poland the data of Agricultural Census indicates that the number of farms with off-farms activity increased. The agrotourist farms are still more and more important in many parts of Poland, especially in the wi tokrzyskie voivodship. The local and voivodship authorities are obliged to improve and invest in agrotourist base, also using the European Union funds. This study is the analysis of natural and social-economic elements of geographical environment in the wi tokrzyskie voivodship. The article is a trial of showing how rural areas are diversified for agrotourism.
5
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Content available

Economic and social consequences of national economic restructuring on examples taken from middle Pomerania

58%
Rydz E.
Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
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1998
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tom 02
6

Human resources and development of the regions

58%
Kuzmisinova V.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
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2003
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tom 87
7

Transformation processes in Lithuanian agriculture

58%
Ramanauskas J.
Roczniki Akademii Rolniczej w Poznaniu. Rolnictwo
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1999
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tom 53
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nr 2
8

Challenges and chances for dairying in the light of the tasks of transformation process of the economy and tendencies on the world milk market

58%
Imbs B.
Annals of Warsaw Agricultural University. Agricultural Economics and Rural Sociology
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1996
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tom 32
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