Wyniki wyszukiwania

1

Mechanisms of transcription regulation

100%

Poluha D.

Applied Biology Communications. Lublin Scientific Center

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1991

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tom 01

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nr 6

2

Natural agents-mediated targeting of histone deacetylases

100%

Farooqi A. A., Kamran-ul-Hassan Naqvi S., Perk A. A., Yanar O., Tabassum S., Ahmad M. S., Mansoor Q., Ashry M. S., Ismail M., Naoum G. E., Arafat W. O.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

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nr 1

3

Regulation of RNA polymerase III transcription by Maf1 protein

100%

Ciesla M., Boguta M.

Acta Biochimica Polonica

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2008

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tom 55

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nr 2

215-225

Maf1 was the first protein discovered to regulate polymerase III RNA in yeast and because it is evolutionarily conserved, a Maf1 ortholog also serves to restrain transcription in mouse and human cells. Understanding the mechanism of the regulation has been made possible by recent studies showing that Maf1 is a nuclear/cytoplasmic protein whose subcellular distribution and hence negative regulation of Pol III transcription is mediated by the nutrient-sensing signaling pathways, TOR and RAS. Under stress conditions and during growth in a nonfermentable carbon source Maf1 is dephosphorylated and imported to the nucleus. In its non-phosphorylated form, Maf1 interacts with the polymerase III transcription machinery. Phosphorylation serves to locate Maf1 to the cytoplasm under favorable growth conditions, thereby preventing it from non-negatively regulating polymerase III when high levels of tRNA transcription are required. Relocation of Maf1 to the cytoplasm is dependent on Msn5, a carrier responsible for export of several other phosphoproteins out of the nucleus. The absence of Maf1-mediated control of tRNA synthesis impairs yeast viability in nonfermentable carbon sources. Moreover, in cells grown in a nonfer mentable carbon source, Maf1 regulates the levels of different tRNAs to various extents. This differential regulation may contribute to the physiological role of Maf1.

4

Arabidopsis class A and B HSFs show a spectrum of transcriptional activity

100%

Czarnecka-Verner E., Gurley W. B.

Biotechnologia

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2002

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nr 3

5

Non-random distribution of GATC sequences in regions of promoters stimulated by the SeqA protein of Escherichia coli

100%

Strzelczyk B., Slominska-Wojewodzka M., Wegrzyn G., Wegrzyn A.

Acta Biochimica Polonica

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2003

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tom 50

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nr 4

The SeqA protein of Escherichia coli is not only the main negative regulator of DNA replication initiation but also a specific transcription factor. It binds to hemi- methylated GATC sequences and, with somewhat different specificity, to fully methylated GATC regions. Recently, a microarray analysis was reported, in which transcriptomes of wild-type and AseqA strains were compared. Although in the ΔseqA mutant the levels of some transcripts were significantly decreased while certain transcripts were evidently more abundant relative to wild-type bacteria, no correlation between the presence of GATC motifs in promoter sequences and transcription activity was found. However, here we show that when larger DNA fragments, encompassing positions from -250 to +250 relative to the transcription start site, are analyzed, some common features of GATC distribution near the promoters activated by SeqA can be demonstrated. Nevertheless, it seems that the GATC pattern is not the only determinant of SeqA-dependence of promoter activity.

6

Nuclear receptors, their coactivators and modulation of transcription

100%

Manteuffel-Cymborowska M.

Acta Biochimica Polonica

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1999

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tom 46

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nr 1

Nuclear receptors arc ligand-dependent transcription factors which can also be activated in the absence of their lipophilic ligands by signaling substances acting on cell membrane receptors. This ligand-independent activation indicates the importance of nuclear receptor phosphorylation for their function. Nuclear receptor- mediated transcription of target genes is further increased by interactions with recruited coactivators forming a novel family of nuclear proteins. CBP/p300, a coactivator of different classes of transcription factors, including the tumor suppressor protein p53, plays a special role acting as a bridging protein between inducible transcription factors and the basal transcription apparatus, and as an integrator of diverse signaling pathways. Coactivators of nuclear receptors and associated proteins forming a multicomponent complex have an intrinsic histone acetylase activity in contrast to nuclear receptor and heterodimer Mad-Max corepressors, which recruit histone deacetylase. Similarly the Rb protein interacts with histone deacetylase to repress transcription of cell cycle regulatory genes. Targeted histone acetylation/dca- cetylation results in remodeling of chromatin structure and correlates with activation/repression of transcription. Recent data point to the important role of coactivator proteins associated with inducible transcription factors in transcription regulation, and in the integration of multiple signal transduction pathways within the nucleus.

7

BH3-only proteins: the lords of death

100%

Fleischer A., Rebollo A., Ayllon V.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 1

8

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Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt

84%

Ortiz-Espin A., Iglesias-Fernandez R., Martinez-Alcala I., Calderon A., Camejo D., Sevilla F., Carbonero P., Jimenez A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

9

The role of WRKY transcription factors in plants

84%

Grzechowiak M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2014

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tom 95

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nr 3

10

The transcriptional regulation of podocin [NPHS2] by Lmx1b and a promoter single nucleotide polymorphism

84%

Harendza S., Stahl R. A. K., Schneider A.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 4

679-691

Podocin (NPHS2) is a component of the glomerular slit membrane with major regulatory functions in the renal permeability of proteins. A loss of podocin and a decrease in its resynthesis can influence the outcome of renal diseases with nephrotic syndrome, such as minimal change glomerulonephritis, focal segmental glomerulosclerosis (FSGS) and membranous nephropathy. The transcriptional regulation of podocin may play a major role in these processes. We defined the transcriptional regulation of the human podocin gene and the influence of single nucleotide polymorphisms (SNPs) within its promoter region in the podocytes using reporter gene constructs and gel shift analysis. In addition, we took genomic DNA from healthy Caucasian blood donors and from biopsies of kidneys with defined renal diseases and screened it for podocin promoter SNPs. Our data shows that the transcription of podocin is mainly regulated by the transcription factor Lmx1b, which binds to a FLAT-F element and displays enhancer function. With the SNP variant −116T, there was a significant reduction in luciferase activity, and nuclear protein binding was observed, while the SNP −670C/T did not display functionality. The allelic distribution of −116C/T in patients with kidney diseases leading to nephrotic syndrome was not significantly different from that in the control group. Our data indicates that among other factors, podocin is specifically regulated by the transcription factor Lmx1b and by the functional polymorphism -116C/T. However, there is no association between −116C/T and susceptibility to minimal change glomerulonephritis, focal segmental glomerulosclerosis or membranous nephropathy.

11

Distribution of transcription factor binding sites along 5'-upstream sequences of genes: estimation of minimal promoters and upstream regulatory regions

84%

Malewski T., Glazko G., Sazanov A.

Biotechnologia

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2004

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nr 3

12

GATA-1 binding to the alphaV promoter negatively regulates expression of the integrin alphaV subunit in human leukemic K562 cells

84%

Czyz M., Stasiak M., Boncela J., Cierniewski C. S.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αv promoter region and are directly involved in the regulation of transcriptional activity of the αv gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αv expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the «v gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced αv mRNA and αv protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αv. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the αv gene promoter and negatively regulates αv gene expression.

13

Histone deacetylase complexes: implications for plants

84%

Lawit S. J., Czarnecka-Verner E.

Biotechnologia

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2002

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nr 3

14

Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours

67%

Piccinni E., Chelstowska A., Hanus J., Widlak P., Loreti S., Tata A. M., Augusti-Tocco G., Bianchi M. M., Negri R.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

In order to understand better the role of the human Tip60 complex component Gas41, we analysed its expression levels in brain tumours and searched for possible interactors. Two-hybrid screening of a human foetal brain library allowed identification of some molecular interactors of Gas41. Among them we found n-Myc transcription factor. The interaction between Gas41 and n-Myc was validated by pull-down experiments. We showed that Gas41 is able to bind both n-Myc and c-Myc proteins, and that the levels of expression of Gas41 and Myc proteins were similar to each other in such brain tumors as neuroblastomas and glioblastomas. Finally, in order to identify which region of Gas41 is involved in the interaction with Myc proteins, we analysed the ability of Gas41 to substitute for its orthologue Yaf9 in yeast; we showed that the N-terminal portions of the two proteins, containing the YEATS domains, are interchangeable, while the C-terminal portions are species-specific. In fact we found that Gas41 C-terminal portion is required for Myc protein interaction in human.

15

Phylogenetic origin and transcriptional regulation at the post-diauxic phase of SPI1 in Saccharomyces cerevisiae

67%

Cardona F., Li Del Olmo M., Aranda A.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

The gene SPI1, of Saccharomyces cerevisiae, encodes a cell wall protein that is induced in several stress conditions, particularly in the postdiauxic and stationary phases of growth. It has a paralogue, SED1, which shows some common features in expression regulation and in the null mutant phenotype. In this work we have identified homologues in other species of yeasts and filamentous fungi, and we have also elucidated some aspects of the origin of SPI1, by duplication and diversification of SED1. In terms of regulation, we have found that the expression in the post-diauxic phase is regulated by genes related to the PKA pathway and stress response (MSN2/4, YAK1, POP2, SOK2, PHD1, and PHO84) and by genes involved in the PKC pathway (WSC2, PKC1, and MPK1).

16

Molecular mechanisms of retinoid action

67%

Gronemeyer H., Miturski R.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 1

17

Plant heat shock transcription factors: divergence in structure and function

67%

Czarnecka-Verner E., Gurley W. B.

Biotechnologia

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1999

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nr 3

18

A common cis-element in promoters of protein synthesis and cell cycle genes

67%

Wyrwicz L. S., Gaj P., Hoffmann M., Rychlewski L., Ostrowski J.

Acta Biochimica Polonica

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2007

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tom 54

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nr 1

Gene promoters contain several classes of functional sequence elements (cis elements) recognized by protein agents, e.g. transcription factors and essential components of the transcription machinery. Here we describe a common DNA regulatory element (tandem TCTCGCGAGA motif) of human TATA-less promoters. A combination of bioinformatic and experimental methodology suggests that the element can be critical for expression of genes involved in enhanced protein synthesis and the G1/S transition in the cell cycle. The motif was identified in a substantial fraction of promoters of cell cycle genes, like cyclins (CCNC, CCNG1), as well as transcription regulators (TAF7, TAF13, KLF7, NCOA2), chromatin structure modulators (HDAC2, TAF6L), translation initiation factors (EIF5, EIF2S1, EIF4G2, EIF3S8, EIF4) and previously reported 18 ribosomal protein genes. Since the motif can define a subset of promoters with a distinct mechanism of activation involved in regulation of expression of about 5% of human genes, further investigation of this regulatory element is an emerging task.

Ograniczanie wyników

Mechanisms of transcription regulation

Natural agents-mediated targeting of histone deacetylases

Regulation of RNA polymerase III transcription by Maf1 protein

Arabidopsis class A and B HSFs show a spectrum of transcriptional activity

Non-random distribution of GATC sequences in regions of promoters stimulated by the SeqA protein of Escherichia coli

Nuclear receptors, their coactivators and modulation of transcription

BH3-only proteins: the lords of death

Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt

The role of WRKY transcription factors in plants

The transcriptional regulation of podocin [NPHS2] by Lmx1b and a promoter single nucleotide polymorphism

Distribution of transcription factor binding sites along 5'-upstream sequences of genes: estimation of minimal promoters and upstream regulatory regions

GATA-1 binding to the alphaV promoter negatively regulates expression of the integrin alphaV subunit in human leukemic K562 cells

Histone deacetylase complexes: implications for plants

Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours

Phylogenetic origin and transcriptional regulation at the post-diauxic phase of SPI1 in Saccharomyces cerevisiae

Molecular mechanisms of retinoid action

Plant heat shock transcription factors: divergence in structure and function

A common cis-element in promoters of protein synthesis and cell cycle genes