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Growth of Staphylococcus aureus strain No 743 and its production of entero- toxin A (ent. A), thermonuclease (TNase) and ß-hemolysin were studied in cheese whey containing zero, 5, 10, 15, and 20% salt during incubation at 37°C for 48 h. Results revealed that the concentration of salt less than 10% promotes S. aureus growth and production of ent. A, TNase and ß-hemolysin. In the presence of high concentrations of salt (15-20%), growth was inhibited and ent. A production was incompletely repressed, while TNase and ß-hemolysin activities were completely inhibited. It is concluded that using unsalted or salted cheese whey containing S. aureus in preparing any other kind of food related commodity is dangerous. Furthermore, TNase and ß-hemolysis tests are unreliable as indicators for tracing enterotoxin A in dairy products containing more than 10% salt.
The incidence of Bacillus cereus, psychrotrophic character and the ability of isolates to produce haemolysin were investigated to evaluate their health potential in some dairy products. In total 125 samples (skim milk powder, white soft cheese, processed cheese, Kareish cheese and rice with milk) were analysed. Of these (39.2%) contained B. cereus. The viability of (reference and isolated strains) B. cereus and toxin production in sterilized milk was examined during storage at 10°C for 7 days. The two tested strains, when inoculated in milk with 105 cfu/mL, were shown to be capable of producing toxin at the end of the storage period. The antimicrobial activity of 7 strains of lactic acid bacteria against B. cereus was tested to select the effective starter to control the pathogen. Lactobacillus reuteri followed by Lb. rhamnosus were the most effective probiotic cultures. The choice was a mixed culture of Lactococcus lactis ssp. diacetylactis as a starter culture and Lb. rhamnosus as a probiotic culture (1:1) to use in manufacture of Tallaga cheese. The use of this starter resulted in reduction of viable count of B. cereus and so, no toxin was detected in these cheeses. In contrast, in the control cheese (inoculated with 105 cfu isolated strain of B. cereus), the viable counts of B. cereus increased and released detectable amount of enterotoxin at the end of refrigerated storage.
In the presented work the influence of mixture of Propionibacterium freudenreichii and Lactobacillus rhamnosus cultures and supernatants on growth and mycotoxins production by Fusarium culmorum were investigated. The effect of tested mixtures on the fungus growth was evaluated by the plating method. F. culmorum was more sensitive on cultures mixture than supernatants mixture. In the second part of this work the influence of cultures and supernatant mixtures on production of the most important mycotoxins by F. culmorum in grain was estimated. It was found that the concentration of zearalenone was significantly lower in the presence of culture mixtures and supernatant mixture applied in the amount 2 ml into sample. The concentration of nivalenol was on the same level in the majority of trials. Only in the sample with 2 ml of supernatant mixture the amount of nivalenol was significantly lower in comparison with the control. Unexpectedly the deoxynivalenol concentration was significantly higher in almost all samples with cultures and supernatants mixtures.
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