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The content of total lipids and lipofuscins was determined in leaves of S. latifolium aerial-aquatic and terrestrial plants at the flowering and fruiting phases. There were no significant differences in the lipid total content between aerial-aquatic and terrestrial plants at the flowering phase. At the fruiting phase, the lipid content was lower in terrestrial plants. Lipofuscins, the so-called senescence pigments, were determined in plants of both ecotypes at the flowering phase, although visible symptoms of senescence were absent. At the fruiting phase, there was an accumulation of lipofuscins in aerial-aquatic and terrestrial plants that may be explained by progressive senescence. The lipofuscin content was higher in terrestrial plants at both phases of ontogenesis. We concluded that earlier and more intensive senescence is typical for terrestrial plants, which are in the conditions of chronic moderate water deficit on the riverside.
Lipid content and fatty acids composition of non-predatory fish: roach, Rutilus rutilus (L.), bream, Abramis brama (L.), vendace, Coregonus albula (L.), and of predatory fish: Eurasian perch, Perca fluviatilis L., pike, Esox lucius L. and burbot, Lota lota (L.) were examined. These fish were caught from three lakes of Mazurian Great Lakes (Kisajno, Dargin, Niegocin). The content of total lipid and some fatty acids varied widely within and among species. Generally, the lipid content was low (0.56-2.78%). Among the saturated and monounsaturated fatty acids, the predominant fatty acids were palmitic C16:0 (19.24-33.44%), stearic C18:0 (4.37-6.87%), palmitoleic C16:1 (4.51-12.93%), and oleic C18:1 n-9 (6.85-14.49%). Arachidonic C20:4 n-6 (3.17-6.55%), eicosapentaenoic C20:5 n-3 (4.14-8.91%), and docosahexaenoic C22:6 n-3 (5.91-24.67%) acids were the most abundant polyunsaturated fatty acids. In the case of all the freshwater fish, with the exception of bream, higher contents of saturated fatty acids than monounsaturated fatty acids were noted. Among the fish studied, the highest value of total n-3 polyunsaturated fatty acids (43.86%) was noted in vendace, whereas bream contained the highest content of total n-6 polyunsaturated fatty acids (11.21%). The ratio of n-3/n-6 ranged between 1.50 (burbot) and 4.40 (vendace). Differences in the content of fatty acids in fish with different feeding strategies (non-predatory and predatory) were measured. Non-predatory fish were found to have lower values of saturated fatty acids than predatory fish (P mniejsze równe 0.05). Non-predatory fish contained significantly more eicosapentaenoic acid (EPA) than predatory fish (P mniejsze równe 0.01), whereas the amounts of monounsaturated and n-3 and n-6 polyunsaturated fatty acids in non-predatory and predatory fish were not statistically significant (P > 0.05). The total n-3 polyunsaturated fatty acids was higher in non-predatory fish, but not statistically significant (P > 0.05). Similarly, the differences in DHA and n-3/n-6 ratio in muscles of predatory and non-predatory fish were not statistically significant (P > 0.05).
The total lipid contents and iodine value of fats had been determined in fish from two sections of the Zaporizke Reservoir (Ukraine) with different contamination levels. Research was conducted using the muscle and liver tissue samples from pike-perch (Sander lucioperca), european perch (Perca fluviatis), prussian carp (Carassius gibelio), roach (Rutilus rutilus) and rudd (Scardinius erythrophthalmus). Obtained data showed that at the contaminated zone, the total lipid contents were significantly (p ≤ 0.05) reduced in muscle tissue of pike-perch and european perch in comparison with the samples from “conventionally clean” lower section of the reservoir. Increased iodine value of fats in muscle tissue and liver tissue were detected in the predatory fish and both fish groups respectively. The research results could be used for estimation of the adaptation processes in freshwater fish as well as for indication of environmental contamination level in the natural and artificial reservoirs.
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