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Viral cross-protection in plants is a phenomenon, where a mild virus isolate can protect plants against damage caused by a severe challenge isolate of the same virus. It has been used on a large scale in cases where no resistant plants are available. We examined differences in cross-protection between pathotypes of Pepino mosaic virus representing Chilean 2 genotype. The potential of a mild PepMV-P22 isolate to protect tomato against more aggressive challenge isolates causing yellowing and necrotic symptoms was established. The challenge isolates were PepMV-P5-IY (yellowing), PepMV-P19 (necrotic) and PepMV-P22 K67E (artificial necrotic mutant of PepMV-P22 which differ from PepMV-P22 only by a point mutation). Efficient cross-protection was obtained using mild PepMV-P22 against PepMV-P5-IY. After a challenge inoculation with PepMV-P19 or PepMV-P22 K67E symptoms severity were significantly reduced in comparison to non-protected plants; however, necrotic symptoms appeared two months after coinfection. The real-time PCR analysis revealed that the level of accumulation of the necrotic isolate in tomato plants was even 5–7 times higher than that of PepMV-P22.
Stomata begin to close within 24 h of imposing soil flooding. We investigated whether the stomatal response was triggered by reduced photosynthetic efficiency in young, fully expanded leaves of flooded plants. Chlorophyll fluorescence measurements indicated that ФpsII the effective quantum yield of PS II,decreased after stomata began to close in flooded plants. Changes in qP mirrored those of ФpsII, ФpsII was not affected by daytime patterns of stomatal conductance in well-drained plants but was reduced by stomatal closure in flooded plants. Fv/Fm a measure of the overall photosynthetic efficiency of dark- adapted plants, decreased after 57 h of flooding. Therefore, prolonged soil flooding adversely affected the thylakoid membranes. QN, a measure of the amount of captured energy dissipated as heat and therefore, unused by the photosynthetic machinery, began to increase after 32 h of flooding and continued to rise thereafter. The interdependence of the changes in chlorophyll fluorescence parameters and the flooding-induced closure of stomata is discussed.
Based on the amount and duration of external temperature, degree day values are determined according to whether the selected equilibrium temperature is lower or higher than the air temperature. Degree day values are calculated according to the fluctuations in ambient temperature. It can be understood whether heating or cooling systems are necessary within the calculated periods. In this study, the meteorological data of Antalya region dating back many years and the temperature values suggested according to the cultivation periods of tomato in greenhouse environment have been used as material. The daily average temperature values recorded for many years have been compared with the equilibrium temperature values selected for the tomato plant thereby calculating the heating and cooling day values for each cultivation period. Tomato cultivation in greenhouses is carried out in 2 periods in the region in spring and autumn. At the end of these two periods, it was determined that the heating degree days in autumn were determined to be higher than that in spring. Whereas cooling degree days were determined to be greater for autumn cultivation in comparison with that of spring cultivation. Information regarding the energy consumption of an agricultural structure can be obtained beforehand via the heating and cooling degree day values. It has been surmised that such studies will help in making estimations regarding the energy need of various agricultural structures as well as gaining insight in the planning of the design stages that are directly related to the energy consumption of the building such as heating, cooling and ventilation.
Tomato is well known regarding its quality and nutritional value in all over the world but imbalances of fertilizer nutrients severely affect the quality of tomato. To investigate the specific contribution of potassium to yield and quality of tomato, a field experiment was conducted on two tomato cultivars, Nagina and Roma. Foliar application with varying levels (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0%) of potassium solutions was applied to the plants and compared with control (without K). Exogenous application of 0.6% K significantly improved plant height, lycopene content, potassium, fruit weight and diameter. Exogenous application of 0.5, 0.6 and 0.7% K maximally improved ascorbic acid contents of both tomato cultivars whereas 0.4 and 0.8% did not improve ascorbic acid contents. Due to positive correlation between K nutrition and fruit quality attributes, exogenous application of an appropriate K level can contribute to higher yield and better quality of tomato fruits. Among all potassium levels, 0.5–0.7% K maximally improved performance of tomato plants of both cultivars.
In an experiment conducted in 2012-2013, tomatoes were grown in coco coir inoculated with mycorrhizal fungi, in a heated plastic tunnel in the garden of the Research and Experimental Station of the University of Warmia and Mazury in Olsztyn. The experimental materials comprised seedlings of three tomato cultivars, ‘Torero F1’, ‘Growdena F1’ and ‘Listell F1’, supplied by a horticultural farm. The second experimental factor was inoculation of the plant growth medium with endomycorrhizal fungi from the genus Glomus. The aim of this study was to determine the chlorophyll content of leaves of three tomato cultivars grown in coco coir inoculated with mycorrhizal fungi. Each treatment consisted of seven coco coir mats (100 x 20 x 7.5 cm) with four tomato plants grown in each mat, pruned to produce 23 clusters. The experiment was carried out in triplicate. Seedlings, planted in rockwool cubes, were initially placed next to the openings made in the coir mats. Immediately before planting, each seedling was supplied with 10 ml of a standard working solution containing mycorrhizal fungi. The mycorrhizal inoculum was applied directly to the coco coir. Mycorrhizal fungi were not used in the control treatment. The results showed that, in most treatments, SPAD readings recorded in the fall were lower than in the summer, and the noted values were similar to those determined at the beginning of the growing season. The mycorrhizal inoculum improved the nutritional status of tomato plants determined based on the leaf greenness index. The mycorrhizal inoculum increased the yields of all analysed tomato cultivars.
Tomato plants were cultivated (from 2 to 23 days after germination) in media with NO3-, NH+, or a mixture of both forms in different proportions used as the N source given with or without 5 mol dm-3 HCO3-. The accumulation of soluble sugars (reductng sugars and sucrose) and free amino acids was higher in the roots and leaves of NH+-fed plants than in NO3--fed plants. Starch accumulation in NH4+-fed plants was higher in leaves (about 28 %) and lower in roots (about 37 %) in comparison with that of NO3-- fed plants. Plants cultivated in media containing a mixture of NO3-/NH4+ were characterized by a lower content of sugars and amino acids accumulation in comparison with that in plants fed with NO3- or NH4+. An elevated HCO3- concentration in the rhizosphere stimulated the accumulation of soluble sugars and free amino acids in all the experimental variants. There were only small differences in the starch content.
To study the influence of genetic background on the transformation and regeneration of cultivated tomato plants, hairy root lines of tomato (Lycopersicon esculentum) were obtained by inoculating the hypocotyl explants of three tomato cultivars with the Agrobacterium rhizogenes strain DCAR-2, which harbors the pBI-121 binary vector. The Ri-T-DNA transformation into the plant DNA was confirmed by both of mikimopine and GUS assay analyses. The regeneration efficiency from hairy root explants was assessed. The data indicated that white embryonic calli were formed within two weeks in the presence of 2 mgl-1 2, 4-D plus 0.25 mgl-1 kinetin. Adventitious shoots emerged from the embryonic callus in the presence of 1 mgl-1 GA3 along with 0.5 mgl-1 NAA. The regeneration frequency was higher in the cultivar UC-97, followed by Momotaro and then Edkawi. Molecular confirmation of the integration of the GUS gene into the hairy root-derived plants genomes was done via PCR using GUS-specific primers and also using Southern blotting analysis. Our data shows that regeneration is possible from hairy roots of the cultivated tomato and this system could be used to produce transgenic tomato plants expressing the genes present in Agrobacterium rhizogenes binary vectors.
The tomato yellow leaf curl virus infecting tomato plants in Tanzania is reported to be different from the Old World geminiviruses. A study was initiated to investigate the transmission properties of the virus such as, acquisition feeding time, inoculation feeding time, persistence of virus in the vector, mechanical inoculation, seed and graft transmission. Results obtained indicate that the virus is transmitted persistently by Bemisia tabaci Genn., but neither mechanically - nor seed-transmissible. Minimum acquisition and inoculation time was 30 minutes. It is concluded that the properties of the agent causing the yellow leaf curl symptoms in tomato plants from different regions of Tanzania are similar and mimic those of tomato yellow leaf curl Begomovirus species studied elsewhere.
The response of the Mi-1 gene to different densities of Meloidogyne incognita race 2 was investigated under controlled conditions. Susceptible and resistant tomato seedlings were inoculated with 25, 50, 100, 200, 400, 1000, 2000, 5000 and 10 000 second-stage juveniles of M. incognita. Plants were uprooted 8 weeks after inoculation and the numbers of egg masses and galls on the roots, and second-stage juveniles in 100 g soil per pot were counted. In susceptible plants, there was a correlation between the number of egg masses on roots until 2000 J2 inoculum densities. In resistant plants, when inoculum densities increased, the number of egg masses and galls also increased. The reproduction factor ratio was >1 in the susceptible plant and <1 in the resistant plant. The data showed that the 5000 J2 inoculum was a critical limit, and 10 000 J2s were above threshold for resistant plants. The data indicate that densities of M. incognita can seriously affect the performance of the Mi-1 gene.
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