Wyniki wyszukiwania

1

Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA

100%

Szemraj J., Al-Nedawi K. N. I., Chabielska E., Buczko W., Pawlowska Z.

Acta Biochimica Polonica

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2005

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tom 52

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nr 4

The inhibitory effect of numerous analogues of PO-16, an hexadecadeoxyribonucleotide antisense to sequences -22 to -17 of PAI-1 mRNA coding for a fragment of the signal peptide, on the expression of PAI-1 in endothelial cells, and physiological consequences of the subsequently reduced PAI-1 activity tested in vitro and in vivo, were described in our previous studies. Of particular interest was PO-16 5'-O-conjugated with menthyl phosphorothioate (MPO-16R). In this work, tissue localisation of MPO-16R labelled with [35S] phosphorothioate at the 3'-end, was determined. [35S]MPO-16R and control [35S]MPO-16R-SENSE oligonucleotides were administered intravenously into 22 rats and organ distribution of the labelled bioconjugates was assessed after 24 and 48 h. For this purpose, tissue sections were subjected to autoradiography, and quantitated by liquid scintillation after solubilisation. Overall clearance of radioactivity was already seen after 24 h, with the radioactivity recovered mainly in the kidney and liver. A smaller fraction of radioactivity was also retained in the spleen and heart. The kidney concentration of the labelled probe was higher than that of liver by 50%. The distribution of PAI-1 mRNA in untreated rat kidney, liver, spleen and heart established by two independent techniques: Ribonuclease Protection Assay and Real-Time PCR, shows the same pattern as that observed for [35S]MPO-16R antisense.

2

The role of the tissue plasminogen activator as a prognostic and differentiation factor in patients with pancreatic cancer and chronic pancreatitis

84%

Baluka D., Urbanek T., Lekstan A., Swietochowska E., Wiaderkiewicz R., Kajor M., Jedrzejewska-Szypulka H., Kusnierz K., Lampe P.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 1

3

Haemostatic factors and intraluminal thrombus thickness in abdominal aortic aneurysm. Is secondary fibrinolysis relevant?

84%

Siennicka A., Drozdzynska M., Chelstowski K., Cnotliwy M., Jastrzebska M.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 3

4

Tissue plasminogen activator in the amygdala: a new role for an old protease

67%

Skrzypiec A. E., Buczko W., Pawlak R.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 8

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

1 Journal of Physiology and Pharmacology. Supplement

2 Buczko W.

1 Al-Nedawi K. N. I.

1 Baluka D.

1 Chabielska E.

1 Chelstowski K.

1 Cnotliwy M.

1 Drozdzynska M.

1 Jastrzebska M.

1 Jedrzejewska-Szypulka H.

1 Kajor M.

1 Kusnierz K.

1 Lampe P.

1 Lekstan A.

1 Pawlak R.

1 Pawlowska Z.

1 Siennicka A.

1 Skrzypiec A. E.

1 Swietochowska E.

1 Szemraj J.

1 Urbanek T.

1 Wiaderkiewicz R.

1 2016

1 2013

1 2008

1 2005

Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA

The role of the tissue plasminogen activator as a prognostic and differentiation factor in patients with pancreatic cancer and chronic pancreatitis

Haemostatic factors and intraluminal thrombus thickness in abdominal aortic aneurysm. Is secondary fibrinolysis relevant?

Tissue plasminogen activator in the amygdala: a new role for an old protease