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1

Meiotic gynogenesis in ide (Leuciscus idus L.) induced by high-temperature shock

100%
Kucharczyk D., Luczynski M. J., Szczerbowski A., Targonska-Dietrich K., Krejszeff S., Babiak I.
Archives of Polish Fisheries
|
2004
|
tom 12
|
nr 2
Ide spermatozoa were genetically inactivated using ultraviolet (UV) irradiation. The highest survival of haploid embryos was noted in the group treated with UV for 5 min (dose 1920 J m-2). High-temperature shock influenced the suppression of the second polar body in ide oocytes. The highest survival rate of diploid gynogenotes (below 10%) was observed in groups shocked at 12 min after egg insemination for 3 min. Eggs shocked later in time exhibited lower survival rates.
2

How does isoprene protect plant membranes from thermal shock?

100%
Siwko M. E., Marrink S. J., Mark A. E.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr Suppl.
3

Optimizing conditions for androgenesis induction in koi carp

84%
Kwiatkowski M., Kucharczyk D., Szczerbowski A., Luczynski M. J., Mamcarz A., Jamroz M.
Electronic Journal of Polish Agricultural Universities. Series Fisheries
|
2008
|
tom 11
|
nr 2
4

Artificial gynogenesis in common bream (Abramis brama (L.)) induced by cold-temperature shock

84%
Kucharczyk D., Luczynski M. J., Szczerbowski A., Woznicki P., Luczynski M., Targonska-Dietrich K., Kujawa R. J., Mamcarz A.
Archives of Polish Fisheries
|
2004
|
tom 12
|
nr 2
Bream, Abramis brama (L.), eggs fertilized with genetically inactivated sperm (UV irradiation dose of 1920 J m-2) were exposed to thermal cold shock to produce meiotic gynogenotes. The shock was applied at one-minute intervals from 1 to 10 min after egg insemination. The temperature of the shock was 2.0 ± 0.1°C, and its duration was 45 min. The water temperature prior to the shock was 20.0°C. Eggs fertilized with genetically inactivated sperm (putative haploids) exhibited retarded and abnormal development. The yield of gynogenesis was relatively low, except for the group to which the shock was applied 1 min after fertilization (about 30% in comparison with the controls). Ninety fish from the control and gynogenetic groups were reared for ten months. The survival of the gynogenetic bream was twofold lower than that of the controls. The gynogenotes were highly variable in size and exhibited some morphological abnormalities. The sex ratios in the control groups were close to 1:1, whereas all the gynogenotes were female.
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Induction of multi-nucleate microspores in anther culture of salix viminalis L.

67%
Wojciechowicz M. K., Kikowska M. A.
Dendrobiology
|
2009
|
tom 61
55-64
The aim of the work was to determine how the induction of androgenesis in selected genotypes of Salix viminalis is affected by thermal factors and medium composition. Anthers isolated from male clones of S. viminalis were pre-cultured at 4, 27 and 32°C for two to eight days in liquid Kyo medium with and without the addition of mannitol and on solid MS or WPM medium. The solid media were supplemented with different concentration of disaccharidesand various combinations of growth regulators including kinetin, 2iP, IAA and IBA. Multi-nucleate microspores indicative of androgenesis were observed in anthers pre-cultured for seven days at 4°C in liquid Kyo medium containing mannitol. The rate of androgenesis was higher when the anthers were transferred to solid modified MS medium containing high concentrations of sucrose and kinetin. In studied genotypes of basket willow early uninucleate microspores underwent sporophytic divisions. Ultrastructural observations showed differences in cellular arrangement of pre-stressed microspores. In Salix viminalis, microspores without starch grains and decreased number of lipid bodies were potentially androgenic cells.
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