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1

Mitochondrial uncoupling does not influence the stability of the intracellular signal activating plasma membrane calcium channels

100%
Zablocki K., Makowska A., Duszynski J.
Acta Biochimica Polonica
|
2001
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tom 48
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nr 1
The effects of various concentrations of thapsigargin, a specific inhibitor of Ca2+ -ATPase in the endoplasmic reticulum (ER) membrane, on calcium homeostasis in lymphoidal T cells (Jurkat) were investigated. Preincubation of these cells suspended in nominally calcium-free medium with 0.1 UM thapsigargin resulted in a complete release of Ca2+ from intracellular calcium stores. When the medium was supplemented with 3 mM CaCl2 the cells maintained constantly elevated level of cytosolic Ca2+ . However, tha­psigargin applied at lower concentration produced only a partial depletion of the stores. For example, in the cells pretreated with 1 nM thapsigargin and suspended in calcium-free medium approximately 75% of the calcium content was released from the intracellular stores. The addition of 3 mM CaCl2 to such cell suspension led to a transient increase in cytosolic calcium concentration, followed by a return to a lower steady-state. This phenomenon, related to the refilling of the ER by Ca2+ , allowed to estimate the half-time for the process of cell recovery after activation of store-operated calcium channels. By this ap­proach we have found that carbonyl cyanide m-chlorophenylhydrazone, which has been documented to inhibit calcium entry into Jurkat cells, does not influence the stability of the intracellular signal involved in the activation of store-operated calcium channels.
2

Thapsigargin: potent inhibitor of Ca2 transport ATP-ases of endoplasmic and sarcoplasmic reticulum

100%
Sabala P., Czarny M., Woronczak J. P., Baranska J.
Acta Biochimica Polonica
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1993
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tom 40
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nr 3
3
Content available remote

Effects of thapsigargin on stimulation frequency - dependent changes in mitochondrial calcium in rat cardiac myocytes

100%
Pytkowski B., Lewartowski B.
Journal of Physiology and Pharmacology
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2002
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tom 53
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nr 4,2
We investigated in the single myocytes of rat heart the effect of blocking of ATP-ase of sarcoplasmic reticulum (SR) on mitochondrial Ca2+ uptake and release. Mitochondrial Ca2+ content was investigated as Mn2+ - resistant fluorescence of Indo 1 - AM loaded into cells. SR ATP-ase was blocked with 10-6 M thapsigargin (Tg). Tg blocked almost completely stimulation Œdependent mitochondrial Ca2+ uptake and slowed down its release despite that the maximal cytosolic Ca2+ concentration was not decreased. We propose that mitochondrial stimulation -dependent Ca2+ uptake is greatly enhanced by [Ca2+ ] built by SR in microdomains adjacent to these organelle.
4
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The source of contracticle calcium in guinea-pig cardiac myocytes treated with thapsigargin

100%
Janiak R., Lewartowski B.
Journal of Physiology and Pharmacology
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1996
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tom 47
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nr 3
5
Content available remote

Melatonin influences the expression and oligomerization of amylin in rat INS-1E cells

84%
Jung E.-M., Yoo Y.-M., Jeung E.-B.
Journal of Physiology and Pharmacology
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2019
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tom 70
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nr 5
6
Content available remote

Bastadin 12 and ryanodine reveal similarities between thapsigargin- and tetrabromobisphenol A-induced intracellular Ca2plus release in cultured cerebellar granule cells

84%
Zieminska E., Stafiej A., Toczylowska B., Lazarewicz J. W.
Journal of Physiology and Pharmacology
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2014
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tom 65
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nr 5
7
Content available remote

The effect of sarcoplasmic reticulum Ca2+ leak on contractile activity of guinea pig heart myocytes depends on activity of sarcoplasmic reticulum Ca2+-ATPase and Na+/Ca2+ exchanger

84%
Mackiewicz U., Lewartowski B.
Journal of Physiology and Pharmacology
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2008
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tom 59
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nr 2
Decreased expression of sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA), overexpression of Na+/Ca2+ exchanger (NCX) and diastolic SR Ca2+ leak from the ryanodine receptors (RyRs) are believed to contribute to the decrease of myocyte contraction in failing heart. In this work we induced Ca2+ leak through RyRs in isolated myocytes of guinea pig hearts by 20µM FK-506. The SR Ca2+ leak resulted in (1) decreased amplitude of cell shortening and of Ca2+ transients, (2) decreased rate of Ca2+ transients decay (3) enhanced diastolic Ca2+ loss. The effect of FK-506 on amplitude of cell shortening was reversed and that on diastolic Ca2+ loss blocked by partial inhibition of NCX due to lowering Na+ concentration in superfusion solution from 144 mM to 100 mM. The amplitude of cell shortening and Ca2+ transients decreased by FK-506 was significantly increased by 10-7 M thapsigargin. In conclusion, the effect of SR Ca2+ leak induced by FK-506 on myocyte contraction is strictly dependent on activity of SERCA and NCX.
8
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The effect of thapsigargin on sarcoplasmic reticulum Ca2 content and contractions of single myocytes of rat ventricular myocardium

84%
Lewartowski B., Wolska B.
Journal of Physiology and Pharmacology
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1993
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tom 44
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nr 3
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Sources of activator Ca2plus for galanin-induced contractions of rat gastric fundus, jejunum and colon

84%
Korolkiewicz R. P., Konstanski Z., Rekowski P., Ruczynski J., Szyk A., Korolkiewicz K. Z., Petrusewicz J.
Journal of Physiology and Pharmacology
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2000
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tom 51
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nr 4,2
10
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Thapsigargin inhibits the effects of noradrenaline and high [Ca2]0 on kinetics but not on amplitude of contraction in the single myocytes of guinea pig heart

84%
Janiak R., Lewartowski B.
Journal of Physiology and Pharmacology
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1995
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tom 46
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nr 1
11

Sphingosine stimulates calcium mobilization and modulates calcium signals evoked by thapsigargin in glioma C6 cells

67%
Sabala P., Wiktorek M., Czarny M., Chaban V., Baranska J.
Acta Neurobiologiae Experimentalis
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1996
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tom 56
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nr 2
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of sarcoplasmic reticulum Ca release into diadic region on Na-Ca exchange in cardiac myocytes

67%
Lewartowski B., Janiak R., Langer G. A.
Journal of Physiology and Pharmacology
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1996
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tom 47
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nr 4
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