Wyniki wyszukiwania

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Induction of autotetraploidy in dragonhead [Dracocephalum moldavica L.] by colchicine treatment

100%

Omidbaigi R., Yavari S., Hassani M. E., Yavari S.

Journal of Fruit and Ornamental Plant Research

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2010

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tom 18

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nr 1

23-35

The genome doubling agent colchicine was used effectively to obtain tetraploid plants in dragonhead. Treatment of apical meristem of seedlings was carried out in two stages. The first stage was when the cotyledon leaves emerged. The second stage was when the two true leaves emerged. Six levels of colchicine concentrations: 0, 0.05, 0.1, 0.2, 0.5, and 0.75% were applied in each of these stages. Seedling treatment in the stage of emergence of two true leaves with 0.1% colchicine solution proved to be the most effective in producing autotetraploids. Morphological, cytological and flow cytometry analyses showed the increase of chromosome numbers from 2n=2x=10 to 2n=4x=20. The increase of ploidy levels caused major changes in some morphological and physiological traits and active substances in dragonhead.

2

Cytogenetic investigations of different types of cells from flower buds of Arabidopsis thaliana [L.] Heynh.

100%

Weiss H., Maluszynska J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

3

Increase in frequency of tetraploid plants after fast neutron irradiation of callus Arabidopsis thaliana [L.] Heynh.

100%

Gaj M. D., Maluszynski M.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

4

Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus

100%

Fras A., Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica

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2003

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tom 45

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nr 2

Different explants from diploid and tetraploid plants of Arabidopsis thaliana ecotype Wilna were cultured in vitro on the same medium in the same conditions. The mode and capacity for regeneration and the ploidy level of regenerants were examined in eight callus lines. The explant responses to in vitro conditions depended on the ploidy level of donor plants and on the type of explant. Callus derived from leaves of tetraploid plants showed the highest ability to regenerate. Histological investigation showed that plants regenerated mostly through organogenesis and occasionally through embryogenesis. Somatic embryos were observed more frequently in callus derived from tetraploid plants. Regenerated plants were diploids, triploids and tetraploids. The majority of regenerated plants from callus of diploid origin were diploid, but diploids were also observed quite frequently among regenerants from callus of tetraploid origin.

5

Structural and embryological studies on the diploid and tetraploid Arabidopsis thaliana genotypes

100%

Karcz J., Weiss H., Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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1999

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tom 41

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nr 1

Ograniczanie wyników

2 Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Journal of Fruit and Ornamental Plant Research

3 Maluszynska J.

2 Weiss H.

1 Fras A.

1 Gaj M. D.

1 Hassani M. E.

1 Karcz J.

1 Maluszynski M.

1 Omidbaigi R.

1 Yavari S.

1 2010

1 2003

1 1999

2 1998

Induction of autotetraploidy in dragonhead [Dracocephalum moldavica L.] by colchicine treatment

Cytogenetic investigations of different types of cells from flower buds of Arabidopsis thaliana [L.] Heynh.

Increase in frequency of tetraploid plants after fast neutron irradiation of callus Arabidopsis thaliana [L.] Heynh.

Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus

Structural and embryological studies on the diploid and tetraploid Arabidopsis thaliana genotypes