Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 10

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  test immunofluorescencji
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
There were compared the findings concerning diagnosis of rabies using anti-rabies conjugates, i.e. Czechoslovakian policlonal Bioveta and American monoclonal CENTOCOR. There were evaluated the impression slides stained with Bioveta and CENTOCOR conjugates obtained from 27 foxes, a racoon dog, a stone marten, 4 cats, 2 dogs and a cow. The animals were suspected for rabies. Out of 36 animals rabies was diagnosed in 32 cases with the Bioveta conjugate (88.9%) and only 18 with CENTOCOR (50%). The samples responding positive with the Bioveta and negative with CENTOCOR were assessed by inoculation of mice. The results suggested that the serotypes of rabies virus which were not diagnosed with CENTOCOR FITC could circulate in nature. Thus the CENTOCOR FITC conjugate is of a limited value for diagnostic purposes of rabies.
The monoclonal CENTOCOR FITC conjugate produced by CENTOCOR, Malvern PA 19355, USA has been used for detection of rabies virus in brain impression slides from mice inoculated with Flury LEP strain MICLD₅₀ titer log 5,32. The conjugate has been used at the dilutions 1:10, 1:20, 1:40. For comparison same brain impression slides have been treated with anti-rabies policlonal conjugate made by czechoslovakian company Bioveta (working dilution 1:8). The control slides have comprised the impressions of normal (non-inoculated with rabies) mouse brain labeled with either CENTOCOR undiluted or Bioveta conjugate diluted 1:8. The same complexes emiting bright apple green fluorescence have been found out in impressions labeled with either Centocor or Bioveta conjugates. The CENTOCOR has given the positive reaction in the dilution up to 1:20. The background staining has been markedly reduced in impressions treated with CENTOCOR (contains Evans blue counterstain). The CENTOCOR lot No. 9C0011 may be used in the dilution 1:2 to provide 10-fold excess of antibody recommended by the company.
Parvoviral infections of dogs are still a clinical problem throughout the world. This is despite the development of prevention, increased awareness among the owners and increasingly effective treatment protocols due to the extremely high virulence of the virus. Parvovirosis is a disease caused by a virus of the Parvoviridae family in various variants: CPV-2, CPV-2a, CPV-2b and CPV-2c, all of which produce similar clinical signs, including acute hemorrhagic enteritis and myocarditis. It is one of the most important viral pathogens, with extremely high morbidity and mortality. This study is a compendium of current knowledge about parvoviral infections in dogs. It describes their aetiology, pathogenesis and factors predisposing to parvovirosis. Special emphasis is placed on the description of clinical signs and treatment of sick dogs. Various diagnostic methods that are necessary to make the final diagnosis of the disease are described. The recommendations of the WSAVA on prophylactic vaccination are also analyzed.
The purpose of the paper was to present current knowledge on the epizootiology and diagnosis of respiratory infections caused by BRSV. This virus is one of the major infectious agents causing diseases in young cattle. BRSV can be the main cause of a respiratory infection or it can be one of the etiological agents of respiratory disease complex. The results of serological examinations and virus isolation showed that BRSV is widely distributed in cattle population all over the world. Economic losses caused by BRSV are associated mainly with high costs of treatment, decreased growth rates and calf mortality. A rapid diagnosis of the disease is crucial for further therapeutic management and the treatment should be concentrated on limiting losses within the herd. During recent years several papers concerning the diagnosis of BRSV have been published. This review describes not only the classical methods of virus detection (virus isolation in a cell culture, immunofluorescence, immunohistochemistry) but also the recently applied PCR assay.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.