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1

The intrabody targeting of hTERT attenuates the immortality of cancer cells

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Zhu X., Yang N., Cai J., Yang G., Liang S., Ren D.
Cellular and Molecular Biology Letters
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2010
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tom 15
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nr 1
32-45
hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.
2
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„Neural stem cells puzzle“ missing piece: telomerase reverse transcriptase expression patterns during nervous system ontogenesis in rats

100%
Mochnacky F., Bul'kova V., Holota R., Zrubakova J., Slovinska L., Kiskova T., Daxnerova Z.
Journal of Physiology and Pharmacology
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2021
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tom 72
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nr 6
For almost three decades, neural stem cells remain still up-to-date and enigmatic topic. The main problem for their studying is the non-existence of an exclusive neural stem cell marker and the heterogeneity of them across the nervous system. As one of the novel markers of neural stem/progenitor cells may serve telomerase reverse transcriptase (TERT), a catalytic subunit of the telomerase enzyme, responsible for retaining the cell immortality. Thus, the aim of our study was to reveal if TERT, as an enzyme for ensuring the immortality of proliferating cells, could be used as a potential marker of neural stem/progenitor cells during the ontogenesis of the rat central nervous system. In this study, we used various markers related to neural stem or progenitor cells character and examined their co-localization with TERT expression. Our experiments were performed on the tissue of the brain and spinal cord during several stages of postnatal development and the neural tube during the 14th embryonal day. Cytoplasmatic TERT expression was found in alar plate progenitors and ventral horn neuroblasts of E14 rats. In the postnatal stages of spinal cord ontogenesis, a cytoplasmatic expression in neurons and nuclear expression in astrocytes was defined. In the brain, nuclear TERT expression was found in neural progenitor cells of neurogenic areas. This study provides the first comparative study of TERT expression across the central nervous system ontogenesis. The nuclear presence of TERT may be used as a potential marker of neural stem/progenitor cells, however, further studies are required to confirm these assumptions.
3

The role of alternative splicing in the regulation of telomerase reverse transcriptase [TERT] gene expression in rice [Oryza sativa L.]

100%
Heller-Uszynska K., Kilian A.
Polish Journal of Natural Sciences. Supplement
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2003
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tom 01
4

Differences in the expression of telomerase and prostate-specific membrane antigen in non-advanced prostatic cancer

84%
Gasinska A., Luczynska E., Wilk W., Cichocka A.
Folia Histochemica et Cytobiologica
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2013
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tom 51
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nr 1
5

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

67%
Trapp-Fragnet L., Marie-Egyptienne D. T., Fakhoury J., Rasschaert D., Autexier C.
Cellular and Molecular Biology Letters
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2012
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tom 17
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nr 4
The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.
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