Wyniki wyszukiwania

1

The intrabody targeting of hTERT attenuates the immortality of cancer cells

100%

Zhu X., Yang N., Cai J., Yang G., Liang S., Ren D.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 1

32-45

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

2

The role of alternative splicing in the regulation of telomerase reverse transcriptase [TERT] gene expression in rice [Oryza sativa L.]

100%

Heller-Uszynska K., Kilian A.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

3

Differences in the expression of telomerase and prostate-specific membrane antigen in non-advanced prostatic cancer

84%

Gasinska A., Luczynska E., Wilk W., Cichocka A.

Folia Histochemica et Cytobiologica

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2013

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tom 51

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nr 1

4

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

67%

Trapp-Fragnet L., Marie-Egyptienne D. T., Fakhoury J., Rasschaert D., Autexier C.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 4

The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

1 Folia Histochemica et Cytobiologica

1 Polish Journal of Natural Sciences. Supplement

1 Autexier C.

1 Cai J.

1 Cichocka A.

1 Fakhoury J.

1 Gasinska A.

1 Heller-Uszynska K.

1 Kilian A.

1 Liang S.

1 Luczynska E.

1 Marie-Egyptienne D. T.

1 Rasschaert D.

1 Ren D.

1 Trapp-Fragnet L.

1 Wilk W.

1 Yang G.

1 Yang N.

1 Zhu X.

1 2013

1 2012

1 2010

1 2003

The intrabody targeting of hTERT attenuates the immortality of cancer cells

The role of alternative splicing in the regulation of telomerase reverse transcriptase [TERT] gene expression in rice [Oryza sativa L.]

Differences in the expression of telomerase and prostate-specific membrane antigen in non-advanced prostatic cancer

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells