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The ultrastructure of the tegumental cytoskeleton of Fasciola hepatica has been examined following various extraction fixations. Microtubules were found throughout the tegument. They run parallel to the basal and apical tegumental borders, and have a pre-dominant basal-to-apical orientation in the central parts of the distal cytoplasm. Microtubules are also found in the cytoplasmic bridges which connect the distal and perikaryal tegumental parts.
Strobilae of three hymenolepidid tapeworm species: Diorchis elisae, Fimbriaria fasciolaris and Sobolevicanthus gracilis, obtained from experimentally infected domestic ducks, were incubated for 5,10 and 15 min at a temperature of 38°C, with horseradish peroxidase applied as a tracer by which to research macromolecular transport across the tegument. Through the use of an electron microscope, it was indicated that the marked protein was bound at the surface of the microtriches with the co-participation of glycoproteins of the plasma membrane. It was also shown that the protein molecules gathered at the base of the microtriches were transported in vesicular form inside the syncytial layer and perikaryon part of the tegumental cytoplasm. Molecules of horseradish peroxidase liberated from the vesicles were observed in the excretory system of the tapeworms. In spite of interspecific differences in the structure of the tegument, the tracer protein transmission observed in this research was found to proceed in a similar manner in all the hymenolepidids studied.
The tegument of the microphallid digenean Maritrema feliui, examined by means of TEM, is described as a syncytial epithelium organised into two layers. The outer layer is an external anucleate, cytoplasmic region connected to a second region composed of nucleate perikarya (cytons) deeply embedded in the surrounding cortical parenchyma. The surface layer of the tegument is covered by a plasma membrane with many deep invaginations, which are apparently pinocytotic. This layer also bears numerous large, electron-dense spines of two types, which are intracellular and attached to the basal plasma membrane. Its cytoplasm is rich in free ribosomes, contains numerous mitochondria, disc-shaped granules frequently arranged in a rouleau, and several large, moderately electron-dense, membranous bodies. The subtegumentary perikarya and their nuclei, which are both flattened, are described in detail, as are their connections with the surface tegument. These perikarya appear to be the source of the disc-shaped granules and some of the other inclusions present in the surface layer. The main characteristics of the tegumental structure of M. feliui are commented upon in relation to the findings of previous publications and their suggested functions.
Wildolive seeds (Olea europaea L. var. sylvestris), calledoleaster do not germinate when placedunder favourable conditions. In a series of experiments the effects of the harvest date, the endosperm, the tegument, andthe coldtreatment were evaluatedon germination of seeds andembryos. The germination percentage of embryos and seeds harvested at different harvest dates increased during October month, these percentages decreased during November month, whereas no seeds and embryos harvested on the middle of December germinated. Embryo germinability was always higher than seed germinability, and this may be due to an inhibiting effect of the teguments and the endosperm on seed germination. Such dormancy, which gradually increased during maturation, could reside mainly in the endosperm and partly within the embryo. The cold treatment at 4°C for four to thirteen days increased seed and embryo germinability, whereas lengthening time at this temperature showeda negative effect on seedgermination. The germination of seeds andembryos from six wildolive trees was also examinedby recording the germination percentage andminimum imbibition time (Tmi).
The tegument ultrastructure of the intestinal fluke Aphallus tubarium was studied for the first time with the use of scanning and transmission electron microscopy. New details on morphology were recorded. The ultrastructural study revealed that the tegument of A. tubarium had a syncytial organization with a distal cytoplasm lying over a basal matrix and cytons. The surface of the tegument is covered with pectinate spines arranged quincuncially. As anterior-posterior differences were observed, particular attention was given to spines. Spines decrease in size and density from the anterior part of body to posterior part. Two types of sensory structures were identified, uniciliated and dome-shaped. Type 1 sensory receptors were outgrowths bearing groups of papillae with shorter and rigid apical seta visible on the anterior part of body surface, encircling the worm. Type 2 sensory receptors was dome-shaped papillae devoid of cilia, found mainly around the oral sucker. Diagrams of spines and sensory receptors were made to help in understanding the nature of these structures. Surface morphology may prove to be useful in distinguishing Aphallus spp with other Cryptogonimidae.
Comparative studies on the tegument of F. fasciolaris (Pallas, 1781) and F. czaplinskii Grytner-Zięcina, 1994, using transmission electron microscopy revealed in both species examined evident differences in the morphology of the tegument surface from various parts of the cestode body (scolex, pseudoscolex, strobila). Two categories of microtriches, characteristic for both studied fimbriariids, were found: (1) typical microtriches with a well developed basal part and an osmiophilic spine at the apex, present on the scolex and on the fan-like, polar side of the pseudoscolex, and (2) non-typical spineless microtriches, present on the small, aporal part of the pseudoscolex and on the whole surface of the strobila. The presence of numerous spiny microtriches on the pseudoscolex perhaps indicates an important role for the peculiar holdfast organ, in supporting the scolex in securing the attachment of fimbriariid cestodes in the host intestine. Details of the tegument ultrastructure of Fimbriaria species are described and compared with other cestodes, and their significance is discussed.
The teguments of four cestode species (Triaenophorus nodulosus, Proteocephalus torulosus, P. percae, P. cernuae) and the intestines of their freshwater fish hosts (Esox lucius, Barbatula barbatula, Perca fluviatilis, Gymnocephalus cernuus) inhabited by bacteria have been investigated with scanning electron microscopy. Most of the bacteria have sizes very similar to nanobacteria (that is, 0.25–0.3 μm in diameter). At least 4 microbial morphotypes were identified. These observations indicate that the normal indigenous microflora in cestodes and their fish hosts, associated with the digestive transport surfaces and consist of bacteria and nanobacteria.
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