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Aspergillus niger FBT1, a local extracellular strain for tannase production, was isolated from soil collected from Matang Mangrove Forest Reserve in Perak, Malaysia. This fungus strain was cultivated in an Erlenmeyer flask under a submerged fermentation system. Medium compositions play a very important role in enhancing enzyme production during fermentation. The production of tannase by A. niger FBT1 increased significantly (95%) when the medium compositions and various process parameters were optimized. Incubation for 72 hours (30EC, pH 7) in medium complemented with sodium nitrate was found optimal. Additional supplementation with tannic acid (2% w/v) as the sole carbon source strongly increased the yield of the enzyme.
The influence of food gums (guar, xanthan, arabic) and carboxymethylcellulose (CMC) on bitterness and astringency of caffeine and tannic acid has been studied. The study was performed in critical concentrations (c*) for particular hydrocolloids as well as for values above and below c*. The ability of hydrocolloids to reduce the astringency and bitterness was evaluated using the method of taste indicator and expressed as a percentage of unreduced sensation. The results indicated that the ability of hydrocolloids to mask the astringency and bitterness was differential and depended both on the concentration and the type of the hydrocolloids used. CMC indicated the highest ability to mask bitterness and astringency among the hydrocolloids. It was found that the ability of these polymers to reduce the astringency was increasing above the c* concentrations.
The effect of protocatechuic acid, tannic acid and trans-resveratrol on the activity of p-nitrophenol hydroxylase (PNPH), an enzymatic marker of CYP2E1, was examined in liver microsomes from acetone induced mice. trans-Resveratrol was found to be the most potent inhibitor (IC50 = 18.5 ± 0.4 uM) of PNPH, while protocatechuic acid had no effect on the enzyme activity. Tannic acid with IC50 = 29.6 ± 3.3 uM showed mixed- and trans-resveratrol competitive inhibition kinetics (Ki = 1 uM and 2.1 uM, respec­tively). Moreover, trans-resveratrol produced a NADPH-dependent loss of PNPH ac­tivity, suggesting mechanism-based CYP2E1 inactivation. These results indicate that trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform.
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