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An improved method for RNA isolation from plants using commercial extraction kits

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Kalinowska E., Chodorska M., Paduch-Cichal E., Mroczkowska K.

Acta Biochimica Polonica

2012

tom 59

nr 3

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Chodorska M.

1 Kalinowska E.

1 Mroczkowska K.

1 Paduch-Cichal E.

1 2012

Wyniki wyszukiwania

An improved method for RNA isolation from plants using commercial extraction kits