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1

Opracowanie testow multiplex PCR do identyfikacji i charakterystyki shigatoksycznych Escherichia coli

100%
Weiner M.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 03
310-313
The aim of the study was to develop multiplex PCR (mPCR) assays which allow identification of shigatoxigenic Escherichia coli (STEC) strains and a characterization of their virulence properties. As an internal control, a fragment of 16S rRNA gene - two amplicons of 230 bp (stx gene) and 401 bp (16S rRNA) were obtained in the test, designated as mPCR-1 for the amplification of the stx gene, characteristic for all known Shiga toxin variants. The mPCR-2 assay was developed to characterize the stx-positive bacterial colonies, which allowed the detection of Shiga toxin 1 and/or 2, the affiliation of STEC O157:H7 serotype (rfbO157 and fliCн₇ genes), and internal control, resulting in amplicons of 348 bp (stx1), 584 (stx2), 420 bp (rfbO157), 247 bp (fliCн₇) and 798 bp (16S rRNA). The detection of markers O26wzx, rfbO111 and rfbO113, typical for E. coli O26, O111 and O113, respectively, was performed with mPCR-3. The products of molecular masses 153, 406 and 593 bp were observed. All STEC’s were also tested using mPCR-4, for the presence of enterohemolysin (ehlyA) and intimin (eaeA) markers generating specific bands of 837 bp (eaeA gene), 534 bp (ehlyA) and 401 (16S rRNA). The mPCR assays developed allow STEC to be identified and characterized and may be used in routine diagnosis of these bacteria.
2

Identyfikacja podstawowych markerow chorobotworczosci shigatoksycznych Escherichia coli metoda PCR z wewnetrzna kontrola amplifikacji

86%
Osek J.
Medycyna Weterynaryjna
|
2004
|
tom 60
|
nr 04
367-370
3

Charakterystyka wybranych markerow patogennosci shigatoksycznych szczepow Escherichia coli wyosobnionych z surowej wolowiny przy uzyciu PCR

86%
Weiner M.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 07
906-911
The aim of the study was the characterization of selected virulence markers of Shiga toxin-producing Escherichia coli originated from raw beef by the use of PCR. The identification of Stx variants (stx₂c, stx₂d, stx₂e and stx₂f) was done with the stx2 -positive E. coli strains, resulting in the detection of the stx2d gene in 13 STEC isolates out of which 3 strains also had the stx₂c gene. None of the isolates possessed the stx2f or stx2e genes. The intimin marker (γ variant) was observed in all E. coli O157:H7, whereas β variant in E. coli O26 isolates. The genotypic factors such as katP, toxB and efa1 were detected in all O157:H7 as well as in one O26 isolate. Fifteen STEC were iha-positive and five saa-positive. None of the saa-positive isolates belonged to the O157 or O26 groups. The simultaneous presence of the lpfAO₁₅₇/OI₋₁₄₁ and lpfAO₁₅₇/OI₋₁₅₄ genes was noted in 8 O157:H7 and in one O26 STEC. The lpfAO₁₅₇/OI₋₁₄₁ gene alone was present in 4 other STEC tested, including one E. coli O26. None of E. coli O157:H7 had the lpfAO113 marker that was observed in the all remaining STEC. In one isolate belonging to the E. coli O26 group, for the first time a simultaneous presence of the lpfAO₁₅₇/OI₋₁₄₁, lpfAO₁₅₇/OI₋₁₅₄ and lpfAO₁₁₃ was noted.
4

Aktualne metody diagnostyki shigatoksycznych szczepow Escherichia coli

86%
Weiner M., Osek J.
Życie Weterynaryjne
|
2003
|
tom 78
|
nr 06
340-342
5

Opracowanie testow multiplex PCR do identyfikacji odmian toksyny Shiga wytwarzanych przez Escherichia coli

86%
Osek J., Weiner M.
Medycyna Weterynaryjna
|
2004
|
tom 60
|
nr 02
207-210
6

Genotypowa charakterystyka szczepow Escherichia coli 0157 izolowanych w Polsce

86%
Osek J.
Medycyna Weterynaryjna
|
2001
|
tom 57
|
nr 04
255-257
7

Genotypowa identyfikacja i roznicowanie shigatoksycznych szczepow Escherichia coli przy uzyciu multiplex PCR

86%
Osek J.
Medycyna Weterynaryjna
|
2001
|
tom 57
|
nr 05
323-326
8

Metoda real-time PCR [r-PCR] w diagnostyce molekularnej shigatoksycznych Escherichia coli

86%
Osek J., Weiner M.
Medycyna Weterynaryjna
|
2004
|
tom 60
|
nr 07
687-691
9

Patogeneza zakazen wywolywanych przez Escherichia coli syntetyzujace toksyny Shiga [STEC]

86%
Twardon J., Sobieszczanska B. M., Gryko R.
Medycyna Weterynaryjna
|
2004
|
tom 60
|
nr 11
1161-1163
10
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Pałeczki okrężnicy - doktor Jekyll czy pan Hyde?

72%
Sobieszczanska B.
Wszechświat
|
2011
|
tom 112
|
nr 07-09
11

Identyfikacja shigatoksycznych Escherichia coli w tuszach wolowych metoda PCR

72%
Osek J.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 02
179-182
E. coli strains producing Shiga toxins (STEC) are the cause of hemorrhagic colitis, hemolytic uremia syndrome (HUS) and thrombotic thrombocytopenic purpura in humans. The source of these bacteria is usually cattle that are asymptomatic carriers of STEC. Infection of humans is usually by the oral route after consuming food of animal origin, especially beef, contaminated with these microorganisms. Monitoring the presence of STEC in animals and food is very important in determining the source of infection as well as in epidemiological analyses. In the present study a method of STEC identification was applied which utilized the enrichment step of the meat samples and then determined the conserved fragment of the stx gene encoding the production of Shiga toxins. For this purpose a multiplex PCR test was established, that allowed amplification of the stx marker as well as the 16S rRNA E. coli gene, which was used as an internal amplification control. As a result of the reaction, two amplicons were obtained of sizes 230 bp (stx) and 401 bp (16S rRNA), respectively when STEC bacteria were present in the tested meat. The study used 221 beef samples obtained from animals directly in slaughter houses, and 13 (5.9%) positive results were obtained. These results may suggest that beef may be a source of STEC, which, in turn, are a potential threat for human health.
12

Molekularne podstawy chorobotworczosci Escherichia coli 0157

72%
Osek J.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 10
750-755
13

Wykorzystanie metody PCR-RFLP do wykrywania i roznicowania shigatoksycznych szczepow Escherichia coli na podstawie analizy genow slt

72%
Nawrotek P.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 01
35-39
14

Shigatoksyczne szczepy Escherichia coli - patogenne dla ludzi bakterie pochodzace od zwierzat

72%
Osek J., Dacko J.
Życie Weterynaryjne
|
2001
|
tom 76
|
nr 08
417-420
15

Wystepowanie i charakterystyka szczepow Escherichi coli grupy 0157 pochodzacych od prosiat z biegunka

72%
Osek J.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 06
433-435
16

Shigatoksyczne szczepy Escherichia coli w infekcjach gruczolu mlekowego u krow

72%
Klossowska A., Malinowski E.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 09
1108-1110
A hundred of E. coli strains from 86 cows with colimastitis were screened using the PCR method for the presence of shiga toxin-producing Escherichia coli (STEC) genes. The procedure involved amplifying the stx gene (stx1, stx2) and its variants (stx2c, stx2d, stx2e, stx2f) with primers described earlier by other authors. The amplification reaction generated PCR products of molecular masses 348, 584, 124, 175, 303, 428 bp, respectively, and 798 bp for 16S rRNA E. coli as an internal control. The presence of shiga toxin genes was found in four strains (4%) and stx2, stx 2c and stx2e genes were stated. The study indicates that mastitis milk can be potentially risky for human since it is an important source of shiga toxin-producing E. coli strains.
17
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Shigatoksyczne enterokrwotoczne szczepy Escherichia coli - nowe czy dobrze znane zagrożenie?

58%
Weiner M.
Życie Weterynaryjne
|
2011
|
tom 86
|
nr 07
18

Zastosowanie specyficznych dla toksyn Shiga sond DNA znakowanych digoksygenina do oznaczania w kale bydla shigatoksycznych szczepow Escherichia coli [STEC]

58%
Osek J., Gallien P.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 11
863-866
19

Patogenne Escherichia coli - mechanizmy chorobotwórczości

58%
Poltorak K., Wieczorek K., Osek J.
Medycyna Weterynaryjna
|
2016
|
tom 72
|
nr 06
E. coli are the predominant microorganisms in the human gastrointestinal tract. In most cases, they exist as harmless comensals, and some of them are beneficial to their host in balancing gut flora and absorption of nutrients. However, there are pathogenic strains that cause a broad range of diseases in humans and animals, from diarrhea to bloodstream infections. Among bacterial strains causing these symptoms, seven pathotypes are now recognized: enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), and adherent-invasive E. coli (AIEC). Several different strains cause diverse diseases by means of virulence factors that facilitate their interactions with the host, including colonization of the intestinal epithelial surfaces, crossing of the mucosal barriers, invasion of the bloodstream and internal organs or producing toxins that affect various cellular processes. Pathogenic E. coli are commonly studied in humans, animals, food and the environment, in developed and developing countries. The presented paper reviews recent information concerning the pathogenic mechanisms of E. coli, the role of animals and food in the transmission chain and a short overview of epidemiological data.
20

Badania nad występowaniem werotoksycznych Escherichia coli w żywności w Polsce

58%
Lopatek M., Wieczorek K., Osek J.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 08
Verotoxin-producing Escherichia coli (VTEC) strains are enteric bacteria which cause severe consequences of food-borne infections in humans. Most of the illnesses caused by VTEC are attributed to serotype O157:H7 and O157:H-. In recent years the strains belonging to non-O157 serogroups, including the highly virulent E. coli O104:H4 strain which was the cause of an epidemic in Germany in 2011, also have an important significance. The present study shows the examination results of samples from cattle carried out between 2004-2010. The investigations were conducted under the direction of VTEC under two monitoring programs. In the first of them, a total of 1,584 samples collected from bovine carcasses as well as beef meat were tested. In the second study, 406 swabs from carcasses and 417 from beef meat were used. It was found that in the first monitoring program 51 (3.2%) samples possessed the vtx gene. In the second project, from 69 positive samples for VTEC obtained after the first PCR reaction only 29 bacterial isolates were received. These strains were used to determine the serotypes. The most frequent verotoxigenic E. coli belonged to serotypes O181:H49 (n = 4), O2:H[32] (n = 4), as well as serogroups O91 (n = 3) and O113 (n = 3). The detection of VTEC, including E. coli O104:H4, in vegetables was based on the real-time PCR method. The examined material consisted of 443 fresh vegetable samples. The vtx1 and vtx2 genes were not identified, which indicates that the analyzed samples were not contaminated with VTEC strains, including O104:H4 serotype.
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