Wyniki wyszukiwania

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Electrophysiological studies on the mechanisms determining the kinetics and pharmacological modulation of GABAergic synaptic transmission

100%

Mozrzymas J. W.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

The subiculum: what it does, what it might do, and what we don't yet know

86%

O'Mara S.

Acta Neurobiologiae Experimentalis

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2005

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tom 65

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nr 5

3

Alteration of sphingolipid biostat in experimental model of oxidative stress evoked by 1-methyl-4-phenylpyridinium (MPPplus)

72%

Pyszko J., Strosznajder J. B.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

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nr 1

Bioactive sphingolipids are important molecules that control wide spectrum of neuronal processes including neurotransmission, synaptic function, cells proliferation and death. Sphingosine kinases (SK1/2) are conserved enzymes that phosphorylate sphingosine to sphingosine-1-phosphate (S1P), which acts as a primary and secondary messenger. S1P binds to 5 receptors and plays essential role in neural signal transduction under physiological and various pathological conditions. Although growing evidence suggests important role of SK1/2 and S1P in neurodegenerative disorders including ischemia, inflammation and Alzheimer’s Disease, till now disturbances of sphingolipids homeostasis in Parkinson’s Disease (PD) remain unknown. Our study try to explain the role of SK1/2 and S1P in molecular mechanism of cell survival and death in model of oxidative stress evoked by neurotoxin 1-methyl-4-phenylpyridinium (MPP+), compound widely used in experimental model of PD. Our data presented that MPP+, comparable to SK inhibition evoked death of human neuroblastoma cells SH-SY5Y in time and concentration dependent manner. These changes are accompanied by increased free radicals concentration in these cells. Reduced level of SK1 protein was detected in SH-SY5Y cells after 24h exposure to MPP+ comparing to control. Moreover S1P pretreatment enhanced survival of these cells and protein level of SK1 comparing to MPP+ treated cells. Our data indicated that MPP+ evoked neuronal death is mediated by SK1/2 inhibition and altered sphingolipids signaling. These molecular events lead to caspase dependent apoptotic cells death and poly(ADP-ribose) polymerase-1 (PARP-1) degradation. All above results presented the alteration of sphingolipid biostat in experimental model of PD and suggested that S1P can offer novel, protective strategy.Supported by NCN Grant 5870/B/PO1/2011/40

4

Estradiol modulates the synapsins phosphorylation by various protein kinases in the rat brain under in vitro and in vivo conditions

58%

Rebas E., Lachowicz L., Lachowicz A.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 1

Synapsins are the neuronal phosphoproteins which play very important role in processes of synaptic neurotransmission. They are physiological substrates for Ser/Thr protein kinases. The reversible phosphorylation of synapsins may be modified by several compounds including steroid hormones. The aim of our study was to investigate, if the one of neuroactive steroid - 17ß-estradiol - could modulate the phosphorylation of synapsins by PKA, CaM-PK and PKC in rat brain and what type of mechanism of their action is possible. The activity of kinases was evaluated as phosphorylation of synapsin in cerebral cortex and hippocampus in vivo and in vitro conditions. We conclude that 17E2 has inhibitory effect on synapsins phosphorylation by all tested kinases in vitro and in vivo conditions. The lack of nuclei in synaptosomal membrane fraction and short time of hormone exposure can be evidence of direct, non-genomic mechanism of estradiol action.

5

Resolving the ionotropic receptor kinetics and modulation in the time scale of synaptic transmission

58%

Pytel M., Mercik K., Mozrzymas J. W.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 1

Synaptic transmission plays a crucial role in signal transduction in the adult central nervous system. It is known that synaptic transmission can be modulated by physiological and pathological processes and a number of factors including metal ions, pH, drugs, etc. The patch-clamp technique allows to measure postsynaptic currents, but the mechanism of these currents modulation remains unclear. The estimated value of neurotransmitter transient indicates that this time course is very short and the activation of postsynaptic receptors is extremely non-equilibrient. The ultrafast perfusion system makes it possible to mimic synaptic conditions and, additionally, the agonist concentration can be controlled, which is very important for pharmacokinetic studies. In the present paper, examples of pharmacological modulation of mIPSC kinetics and currents evoked by ultrafast agonist application are presented.

6

Immediate plasticity of identifiable synapses in the land snails Helix lucorum

58%

Sokolov E., Palikhova T.

Acta Neurobiologiae Experimentalis

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1999

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tom 59

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nr 3

7

Leon L. Voronin [1938-2004] Neuroscientist with the soul of a poet

58%

Bregestovski P., Turlejski K.

Acta Neurobiologiae Experimentalis

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2005

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tom 65

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nr 1

8

Integration of low-frequency sleep oscillations in corticothalamic networks

58%

Amzica F., Steriade M.

Acta Neurobiologiae Experimentalis

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2000

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tom 60

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nr 2

. The corticothalamic system acts as a complex network in promoting the various oscillatory patterns (slow oscillation, spindles, delta) that characterize the state of quiet sleep. Local synchronizing mechanisms of any of the above-mentioned oscillations occur at the site of their genesis, thalamic or cortical. These mechanisms are assisted by the wide-range, synchronized occurrence of the cortical slow oscillation, which finally produces the coalesced picture of slow-wave sleep EEC. Multisite, simultaneous intracellular and field potential recordings in cat, as well as EEC recordings in human were performed in order to assess the state of synchrony and the propagation of various sleep rhythms in the corticothalamic network

9

ATP a potent regulator of inositol phospholipids-phospholipase C and lipid mediators in brain cortex

51%

Strosznajder J., Strosznajder R. P.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 2

10

Involvement of membraneous sialoglycosphingolipids [gangliosides] in the process of transmission and storage of information in the brain of vertebrates

44%

Rahmann H.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

Ograniczanie wyników

Electrophysiological studies on the mechanisms determining the kinetics and pharmacological modulation of GABAergic synaptic transmission

The subiculum: what it does, what it might do, and what we don't yet know

Alteration of sphingolipid biostat in experimental model of oxidative stress evoked by 1-methyl-4-phenylpyridinium (MPPplus)

Estradiol modulates the synapsins phosphorylation by various protein kinases in the rat brain under in vitro and in vivo conditions

Resolving the ionotropic receptor kinetics and modulation in the time scale of synaptic transmission

Immediate plasticity of identifiable synapses in the land snails Helix lucorum

Leon L. Voronin [1938-2004] Neuroscientist with the soul of a poet

Integration of low-frequency sleep oscillations in corticothalamic networks

ATP a potent regulator of inositol phospholipids-phospholipase C and lipid mediators in brain cortex

Involvement of membraneous sialoglycosphingolipids [gangliosides] in the process of transmission and storage of information in the brain of vertebrates