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Early Jurassic aragonitic foraminifers are outstandingly well-preserved in the Marmorea crust, a multiphased ferromanganese layer limiting the Schnöll and Adnet formations (Adnet, Northern Calcareous Alps, Austria). This remarkable preservation, related to the pervasive impregnation of aragonitic tests prior to their recrystallization, allowed observing unknown diagnostic features of the genus Involutina, which typifies the Suborder Involutinina. Thanks to a detailed examination of the Adnet specimens, this paper clarifies the taxonomy, systematic position, and phylogeny of Involutina. A new diagnosis, structural model, and lineage are introduced for the group. Involutina is the direct descendant of Aulotortus and the two taxa probably showed a parallel evolution. As Aulotortus, Involutina presents a high intraspecific variability and its diversity must be revised downward. Current phylogenetic and taxonomic frames of the Suborder Involutinina are firmly questioned as, contrary to previous schemes, the type-genus possesses more than one lamellar deposit per whorl. In Involutina, the height and distribution of papillae on the test surface is not random and probably related to a biological function. We here propose that the papillose lamellae and tube infoldings that characterize representatives of the genus were rudimentary features for light catching and symbiont positioning, respectively.
Plant growth and physiological response of sesame (Sesamum indicum L.) were studied in controlled environment using normal soil and indigenous Vesicular-arbuscular mycorrhiza (VAM) fungi treated soil. The seedlings of Zea mays were inoculated with Giguspora species of VAM (Glomus fasiculatum) and the inoculum was multiplied with help of Zeamays seed bed. Sesame seeds were then inoculated into the bed and it was found that the plant height, shoots lengths, roots, biomass of shoot and roots were considerably increased in the mycorrhizal plants. The effect of VAM infection was assessed in pot experiment. In this comparative study, specific mycorrhizal fungi had consistent effects on various growth parameters such as the number of leaves, number of roots, shoot length, biomass of shoot and roots and biochemical parameters were observed at various time intervals by statistical analysis using two way ANOVA, it was confined with mycorrhizal and non-mycorrhizal infected plants. It was found that the ability of isolates to maintain the plant growth effectively in the case of mycorrhizal seedlings shows a maximum absorbtion of 0.77 ±0.2, shoot length is about 8.34 ±0.2, count of root and leaves are about 8.10 ±0.3, 5.6 ±0.3 respectively under mycorrhizal infection in 30days of analysis and had a positive effect on the growth at all intervals. Biochemical analysis were carried out to estimate the total chlorophyll, chrophyll A, chlorophyll B and Carotenoids contents and it was analyzed to be 9 ±0.5 mg/g, 8.3 ±0.5 mg/g, 3.6 ±0.5 mg/g, 4 ±0.3 mg/g respectively. At the 30th day of analysis for the mycorrhizal plants, it was found to be high in mycorrhizal seedlings which shows the symbiosis had improved the nutrient uptake of cultivated plants. Nevertheless G. fasiculatum was found to be the most efficient fungus and exhibited the highest levels of mycorrhizal colonization, as well as the greatest stimulation of physiological parameters.
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Antiquity of the scleractinian-sipunculan symbiosis

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Extant corals symbiotic with sipunculans, i.e., the caryophylliid Heterocyathus and the dendrophylliid Heteropsammia, develop corallum modifications (in comparison with 'ordinary' representatives of these families) that seem to meet the needs of the coral's worm partner. We distinguish two types of corallum modifications, designated the monoporous and the polyporous types. In the adult monoporous type, the shell inhabited by the sipunculan is usually overgrown only in part by the coral base. There are two orifices: the main one and a smaller pore in the upper part of the corallum. In the polyporous type the shell inhabited by the sipunculan is entirely overgrown and the coral produces a spiralled sipunculan housing. In addition to the main orifice there are several pores in the lower part of the corallum. Heterocyathus priscus sp. n. from the Early Cretaceous (Albian) of France is the oldest example of symbiosis, in which the monoporous-type corallum was modified in the same way as in extant monoporous Heterocyathus. We speculate that the monoporous type was ancestral, as only this type is known to occur among Cretaceous corals. Morphological similiarities between Heteropsammia and certain species of Heterocyathus, such as the Pourtalès plan of septal arrangement and skeleton porosity, may point to a close phylogenetic relationship.
A laboratory study was carried out to evaluate the protective role of ectomycorrhizal fungi against contamination of plants growing in soil treated with cadmium at a dose of 150 ^g Cd/ g soil. An alginate- immobilized inoculum of mycorrhizal fungi was used to introduce the fungi to the soil. The impact of fungi was examined in terms of changes in cadmium levels in inoculated and non-inoculated seedlings of Pinus sylvestris L. It was found that the concentration of cadmium in plants inoculated with fungi was signifi­cantly lower than in non-inoculated seedlings. We also observed that the total concentration of cadmium in contaminated soil inoculated with fungi was lower than in non-inoculated soil.
Respiratory nitrate reductase (NR) from Bradyrhizobium sp. (Lupinus) USDA 3045 has biochemical properties of the membrane-bound NR type. However, in the completely sequenced rhizobium genomes only genes for the periplasmic type of dissimilatory NR were found. Therefore purification and identification of the enzyme by tandem mass spectrometry (MS/MS) was under taken. MS/MS spectra representing 149 unique tryptic peptides derived from purified 137-kDa subunit matched the NCBInr-deposited NarG sequences. MS/MS sequencing of two other SDS/PAGE bands (65- and 59-kDa) identified them as derivatives of the NarH-type protein. Applying additional validation criteria, 73% of the sequence of the NarG subunit (902 aa) and 52% of NarH sequence (266 aa) was assembled (UniProt KB acc. no. P85097 and P85098). This is the first unambiguous identification of an active NarGH-like NR in rhizobia. Moreover, arguments are provided here for the existence of a functional enzyme of this type also among other rhizobial species, basing on immunoblot screening and the presence of membrane-associated NR-active electrophoretic forms.
In this work, clover was shown to respond to infection with Rhizobium leguminosarum bv. trifolii by producing reactive oxygen species. Superoxide radical and hydrogen peroxide were detected in infection threads and nodule primordia. The role of reactive oxygen species in clover-Rhizobium leguminosarum bv. trifolii symbiosis is discussed
Six specimens of a strongly curved, cylindrical hexactinellid sponge have been recovered from the Tommotian– Atdabanian Hetang Biota of South China, and are described as Decumbispongia yuani gen. et sp. nov. The robust, thick−walled sponge shows no evidence of an osculum or basal structures, and the body form is inconsistent with an upright, filter−feeding life position. Interpretations as a detritivore feeding by amoeboid extensions, or as a facultative chemosynthetic symbiosis of sponge and bacteria are considered. The latter interpretation is preferred due to the highly constrained body shape, and the body form is interpreted from this perspective. The species indicates that Cambrian sponges occupied at least some autecological niches that appear to have been vacant since that time.
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A sea slug’s guide to plastid symbiosis

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Some 140 years ago sea slugs that contained chlorophyll-pigmented granules similar to those of plants were described. While we now understand that these “green granules” are plastids the slugs sequester from siphonaceous algae upon which they feed, surprisingly little is really known about the molecular details that underlie this one of a kind animal-plastid symbiosis. Kleptoplasts are stored in the cytosol of epithelial cells that form the slug’s digestive tubules, and one would guess that the stolen organelles are acquired for their ability to fix carbon, but studies have never really been able to prove that. We also do not know how the organelles are distinguished from the remaining food particles the slugs incorporate with their meal and that include algal mitochondria and nuclei. We know that the ability to store kleptoplasts long-term has evolved only a few times independently among hundreds of sacoglossan species, but we have no idea on what basis. Here we take a closer look at the history of sacoglossan research and discuss recent developments. We argue that, in order to understand what makes this symbiosis work, we will need to focus on the animal’s physiology just as much as we need to commence a detailed analysis of the plastids’ photobiology. Understanding kleptoplasty in sacoglossan slugs requires an unbiased multidisciplinary approach.
The establishment of the nitrogen-fixing symbiosis between rhizobia and legumes re quires an ex change of sig nals be tween the two part ners. In re sponse to flavonoids ex creted by the host plant, rhizobia syn the size Nod fac tors (NFs) which elicit, at very low con cen tra tions and in a spe cific man ner, var i ous sym bi otic re sponses on the roots of the le gume hosts. NFs from sev eral rhizobial spe cies have been char ac ter ized. They all are lipo-chitooligosaccharides, con sist ing of a back bone of gen er ally four or five glucosamine residues N-acylated at the non-reducing end, and carrying various O-substituents. The N-acyl chain and the other substituents are important determi nants of the rhizobial host spec i fic ity. A num ber of nodulation genes which spec ify the syn the sis of NFs have been iden ti fied. All rhizobia, in spite of their di ver sity, pos sess conserved nodABC genes responsible for the synthesis of the N-acylated oligo­saccharide core of NFs, which sug gests that these genes are of a monophyletic or i gin. Other genes, the host spe cificnod genes, spec ify the sub sti tu tions of NFs. The cen tral role of NFs and nod genes in the Rhizo bium-legume sym bi o sis sug gests that these fac­tors could be used as mo lec u lar mark ers to study the evo lu tion of this sym bi o sis. We have stud ied a num ber of NFs which are N-acylated by α,β-unsaturated fatty ac­ids. We found that the abil ity to syn the size such NFs does not cor re late with tax o- nomic po si tion of the rhizobia. How ever, all rhizobia that pro duce NFs such nodulate plants be long ing to re lated tribes of le gumes, the Trifolieae, Vicieae, and Galegeae, all of them be ing mem bers of the so-called galegoid group. This sug gests that the abil ity to recognize the NFs with α,β-unsaturated fatty acids is limited to this group of le­gumes, and thus might have ap peared only once in the course of le gume evo lu tion, in the galegoid phy lum.
On the basis of EC6 medium, specific conditions were established to improve its properties as a nutrient medium for isolated, immature zygotic embryos of white clover. The highest frequency of embryo development occurred on medium with 117 mM sucrose concentration and 30% (v/v) coconut water for globular-stage (23.3%) and heart-stage embryos (78.3%). The development of globular-stage embryos was abnormal, however. The embryos callused after two weeks of culture. From the callus produced, plantlets and then plants were regenerated. A double-layer culture system, with the top layer having higher osmolarity than the bottom layer, enabled proembryos smaller than 60 µm to be cultured. For in ovulo embryo culture, Nitsch medium supplemented with 10% (v/v) cucumber juice proved most suitable. On this medium, approximately 13% of the ovules containing few-celled embryos germinated and produced seedlings.
 Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (Common Broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.
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